Humibacillus xanthopallidus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Humibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543H9Y6|A0A543H9Y6_9MICO Short subunit dehydrogenase OS=Humibacillus xanthopallidus OX=412689 GN=FBY41_4484 PE=4 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84VSALLSRR10 pKa = 11.84IAAACAVLLVAALLAPAGSAAAAPVAGEE38 pKa = 3.91MSYY41 pKa = 11.6ACALKK46 pKa = 10.98SNGVLRR52 pKa = 11.84AVSSLSEE59 pKa = 4.18CKK61 pKa = 10.7GNEE64 pKa = 4.01TKK66 pKa = 9.48VTLKK70 pKa = 10.21PGPAIFCVQPSGSTRR85 pKa = 11.84LATKK89 pKa = 10.3PKK91 pKa = 9.68DD92 pKa = 3.89CKK94 pKa = 11.07SPATALTLPPTSGTVYY110 pKa = 10.12FCAALPSGTLRR121 pKa = 11.84YY122 pKa = 8.61VTGPGQCLTGEE133 pKa = 4.44VQVQVTPNDD142 pKa = 3.58AAPSVTSTSPADD154 pKa = 3.74GATHH158 pKa = 6.54VATDD162 pKa = 3.35VSPTVTFSEE171 pKa = 4.74PVTATVGSFAFACDD185 pKa = 3.32ATPIPFAVSGSGGSTLTLDD204 pKa = 3.9PTGSLPQGASCTVTVYY220 pKa = 10.92AAGVTDD226 pKa = 4.75VDD228 pKa = 5.25SLDD231 pKa = 4.2PPDD234 pKa = 4.56NMVANRR240 pKa = 11.84TFSFEE245 pKa = 3.58TDD247 pKa = 3.16AAPSLVSSTPVDD259 pKa = 3.6GAVGVATSTNIVLTFSEE276 pKa = 4.71PVDD279 pKa = 3.69VANGAFSLACGGPALPYY296 pKa = 9.58TVTGSGTSTVTVNPDD311 pKa = 2.69ADD313 pKa = 3.91LAQTATCTLTAPAADD328 pKa = 3.68ITDD331 pKa = 3.59VDD333 pKa = 5.1AGDD336 pKa = 4.22PPNALTAAVDD346 pKa = 3.43ISFQTVDD353 pKa = 3.29AAPTLVSISPADD365 pKa = 3.71GATHH369 pKa = 6.63VATDD373 pKa = 3.82ANIVMTFSEE382 pKa = 4.81PVSVPNAGTFSLEE395 pKa = 4.22CPAGFPQGYY404 pKa = 6.95TVLGLSGTTLTIDD417 pKa = 3.53PAGPLPEE424 pKa = 6.12GEE426 pKa = 4.39TCRR429 pKa = 11.84FTGDD433 pKa = 3.17ASTITDD439 pKa = 3.95NDD441 pKa = 3.77AVDD444 pKa = 4.23PPDD447 pKa = 4.18NLTANIDD454 pKa = 3.22ASFTVDD460 pKa = 3.24SPPAVVSTDD469 pKa = 3.43PADD472 pKa = 3.57NATNVPADD480 pKa = 3.56QEE482 pKa = 4.39LTVTFSEE489 pKa = 4.8PVTVSQSSFTLTCDD503 pKa = 3.35SNPVAFTIGGTDD515 pKa = 3.32TAVTLTPTSALPGTAACLLTVVAAQVSDD543 pKa = 4.14ADD545 pKa = 4.46AGDD548 pKa = 4.47PPDD551 pKa = 3.74TMTANVTVSFTTVDD565 pKa = 3.38TAPTVVSTSPTNGATDD581 pKa = 3.6VASGATITVTFSEE594 pKa = 5.18PVTADD599 pKa = 2.84AGAFSLEE606 pKa = 4.41CPVATTTAFALSGSGTDD623 pKa = 3.16WTLTPDD629 pKa = 3.74AKK631 pKa = 10.91LPAAVLCTLTIDD643 pKa = 3.58GSKK646 pKa = 9.61IHH648 pKa = 7.02DD649 pKa = 4.35TDD651 pKa = 3.79TVDD654 pKa = 4.72PPDD657 pKa = 3.78EE658 pKa = 4.19MVADD662 pKa = 3.74VTVEE666 pKa = 3.74FTVAANSAPTDD677 pKa = 3.81LALSAATVAEE687 pKa = 4.15NQPSGTAVGTFSSTDD702 pKa = 3.68PDD704 pKa = 4.14PGDD707 pKa = 3.47TFTYY711 pKa = 10.18TLDD714 pKa = 4.05AGPGDD719 pKa = 3.88TDD721 pKa = 3.3NGSFTIVGDD730 pKa = 3.91KK731 pKa = 10.8LRR733 pKa = 11.84TAASFDD739 pKa = 3.94FEE741 pKa = 5.14AKK743 pKa = 10.02ASYY746 pKa = 9.91SINVRR751 pKa = 11.84TTDD754 pKa = 2.86SAGNVFEE761 pKa = 4.43KK762 pKa = 10.95SFVITVTDD770 pKa = 3.66VNEE773 pKa = 4.19APTDD777 pKa = 3.47ISLSNATVAEE787 pKa = 4.26NEE789 pKa = 3.95PAATVVGSLTATDD802 pKa = 3.87PDD804 pKa = 3.77AGQSHH809 pKa = 6.85TFTVVTTGCGGTYY822 pKa = 10.61ADD824 pKa = 4.2GSSFTVTGGSLVTSAPLDD842 pKa = 3.79YY843 pKa = 9.98EE844 pKa = 4.49AKK846 pKa = 10.19SSYY849 pKa = 7.43TVCVRR854 pKa = 11.84VTDD857 pKa = 3.48SGTPGLTFDD866 pKa = 4.16EE867 pKa = 5.37LFTITVTNVNEE878 pKa = 4.04APVVGGDD885 pKa = 3.83SYY887 pKa = 11.99SGAIGNTLALRR898 pKa = 11.84GVTATGPSTTLTGALPLANDD918 pKa = 4.09TDD920 pKa = 4.38PEE922 pKa = 5.78GDD924 pKa = 3.87TISVVAGTLATTGGGSVTINANGTFSYY951 pKa = 10.68LPGVGDD957 pKa = 3.66VGQTDD962 pKa = 3.91TVTYY966 pKa = 9.47TVTDD970 pKa = 3.84GLLTSTGTMSIAIGNDD986 pKa = 3.02RR987 pKa = 11.84VWWVNAAAPAGGDD1000 pKa = 3.58GRR1002 pKa = 11.84STAPLTTLTTLNGAGGSGDD1021 pKa = 3.16VDD1023 pKa = 3.71GPGDD1027 pKa = 3.72YY1028 pKa = 11.08LFVYY1032 pKa = 10.01AAAGSYY1038 pKa = 10.58AGGLALEE1045 pKa = 4.68ANQRR1049 pKa = 11.84LHH1051 pKa = 5.52GQRR1054 pKa = 11.84HH1055 pKa = 5.6GLTVGGTVLVPAGATAPTITNAAGNGVTLANGVDD1089 pKa = 3.83VQGVTVSGASADD1101 pKa = 4.41GIHH1104 pKa = 6.93GDD1106 pKa = 3.59NVTTATIGSAVTVSGSGADD1125 pKa = 4.38GIDD1128 pKa = 3.33LTGAATGSIGVAATVTGSTARR1149 pKa = 11.84SVNVSNRR1156 pKa = 11.84SGGTTSFTGPISGTGINLASNTGATVTFSAALTISSGGTSAFSATGGGTVTASDD1210 pKa = 3.71TTSTLTSTTGSTLVVEE1226 pKa = 4.43NTTIGGAGLKK1236 pKa = 9.88FRR1238 pKa = 11.84SISANGAANGIRR1250 pKa = 11.84LNTTGSSGGLSVLGGGNTSLGGNASGGTIQNTTGPGVSLTSTSSVSLNNLTVSNTPGASGIKK1312 pKa = 8.35GTGVAGFSFTYY1323 pKa = 9.05GTVTGSGSAGGGAAGAVASNIAFDD1347 pKa = 3.87GTGDD1351 pKa = 4.21GISNVSGAVTVSNSVLTSGYY1371 pKa = 9.92NHH1373 pKa = 7.48GLTIYY1378 pKa = 10.02NNSGVISTLTVTGNTITSAATAAGSIGSGVLVQEE1412 pKa = 5.04FGSAGTGATITTGSISSNSITGFPNGSGIRR1442 pKa = 11.84LTGGNPTSLAAPVSQIGTSGSHH1464 pKa = 6.45FAITGNLVHH1473 pKa = 6.8GFSTSVLMSTEE1484 pKa = 4.24AIIVLAQGRR1493 pKa = 11.84GTSYY1497 pKa = 11.14IDD1499 pKa = 3.15VTNNGTVANPVGLTQGDD1516 pKa = 4.52GISVNATWQHH1526 pKa = 5.75NLTSAITGNVIAPGTGGVVTNRR1548 pKa = 11.84GITGGAAAGGIPGGTGDD1565 pKa = 3.94TAVLNATISNNTVSQTRR1582 pKa = 11.84GNGIYY1587 pKa = 9.95FVARR1591 pKa = 11.84EE1592 pKa = 4.02SSTLRR1597 pKa = 11.84TTVQNNSVAAPTLIGAGIRR1616 pKa = 11.84FDD1618 pKa = 4.08SGTPAPNSVNATLCALISGNTTAGFSPNPGIALRR1652 pKa = 11.84KK1653 pKa = 9.28EE1654 pKa = 4.25GTVSNVNTFGIFGLSPSPTTTALEE1678 pKa = 4.3TTSYY1682 pKa = 11.37VATQNPASSGGALLVSAQTGFTACTPP1708 pKa = 3.79

Molecular weight: 166.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543H8F6|A0A543H8F6_9MICO Polyketide cyclase/dehydrase/lipid transport protein OS=Humibacillus xanthopallidus OX=412689 GN=FBY41_4617 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4527

0

4527

1567071

29

8685

346.2

36.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.34 ± 0.049

0.675 ± 0.009

6.316 ± 0.032

5.309 ± 0.043

2.733 ± 0.025

9.297 ± 0.04

2.252 ± 0.019

3.552 ± 0.029

1.852 ± 0.027

10.027 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.759 ± 0.017

1.748 ± 0.027

5.82 ± 0.029

2.717 ± 0.019

7.552 ± 0.054

5.696 ± 0.033

6.505 ± 0.057

9.364 ± 0.036

1.606 ± 0.016

1.878 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski