Gordonia phage Moosehead

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5FSJ3|A0A7D5FSJ3_9CAUD Uncharacterized protein OS=Gordonia phage Moosehead OX=2743987 GN=55 PE=4 SV=1
MM1 pKa = 7.93PEE3 pKa = 4.51FKK5 pKa = 10.03PKK7 pKa = 10.4RR8 pKa = 11.84GIDD11 pKa = 3.28LGGEE15 pKa = 4.05FNGDD19 pKa = 3.68FFQWLMGVPNPYY31 pKa = 9.25TGEE34 pKa = 4.39DD35 pKa = 3.36AWQMMQRR42 pKa = 11.84EE43 pKa = 4.56AAGEE47 pKa = 3.94PLPPLKK53 pKa = 10.53VQPDD57 pKa = 3.51LSDD60 pKa = 3.21ARR62 pKa = 11.84NPDD65 pKa = 4.14PYY67 pKa = 9.82TGAMFRR73 pKa = 11.84AEE75 pKa = 4.24YY76 pKa = 9.86PGLKK80 pKa = 9.56PGDD83 pKa = 3.77MCPSCEE89 pKa = 5.27DD90 pKa = 3.42EE91 pKa = 5.46DD92 pKa = 4.7SPISIGEE99 pKa = 4.09DD100 pKa = 3.91GVCYY104 pKa = 10.52LCVCGCSYY112 pKa = 11.11CEE114 pKa = 4.64PDD116 pKa = 4.17DD117 pKa = 3.84AA118 pKa = 7.27

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5JU65|A0A7D5JU65_9CAUD DNA methylase OS=Gordonia phage Moosehead OX=2743987 GN=64 PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84LISKK6 pKa = 9.76HH7 pKa = 5.59AFRR10 pKa = 11.84EE11 pKa = 4.14YY12 pKa = 10.06MKK14 pKa = 10.68FRR16 pKa = 11.84GMTNEE21 pKa = 3.68SLARR25 pKa = 11.84EE26 pKa = 4.42AKK28 pKa = 10.24CAVSTIAFLRR38 pKa = 11.84SRR40 pKa = 11.84GKK42 pKa = 10.38AGRR45 pKa = 11.84DD46 pKa = 3.34SVGSDD51 pKa = 2.73TAQRR55 pKa = 11.84IEE57 pKa = 4.17RR58 pKa = 11.84ALNAPPGSLFSAQVIVASSISNKK81 pKa = 9.92RR82 pKa = 11.84GAAA85 pKa = 3.37

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13757

38

1448

181.0

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.652 ± 0.599

0.996 ± 0.155

6.971 ± 0.282

5.932 ± 0.287

2.66 ± 0.203

8.81 ± 0.41

2.21 ± 0.248

4.805 ± 0.164

3.395 ± 0.242

7.196 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.123

3.038 ± 0.177

5.699 ± 0.263

3.395 ± 0.263

7.458 ± 0.355

5.633 ± 0.267

6.353 ± 0.362

7.073 ± 0.368

2.159 ± 0.15

2.297 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski