Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) (Clostridium sordellii)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Paeniclostridium; Paeniclostridium sordellii

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0E907|T0E907_PAES9 HNH endonuclease family protein OS=Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) OX=1292036 GN=H477_5960 PE=4 SV=1
MM1 pKa = 7.61IKK3 pKa = 9.35IDD5 pKa = 3.68QTNPIDD11 pKa = 4.74DD12 pKa = 4.29DD13 pKa = 4.76FIFEE17 pKa = 3.94KK18 pKa = 10.32SYY20 pKa = 11.56YY21 pKa = 9.1EE22 pKa = 4.07STVTTFSSSNNIVSTKK38 pKa = 10.05SVSSTTVVGRR48 pKa = 11.84STPLVGLPDD57 pKa = 3.93ICPQNWDD64 pKa = 3.3VKK66 pKa = 10.24TFDD69 pKa = 4.23DD70 pKa = 4.81QYY72 pKa = 11.04STLAFQGTWNIQMATPTYY90 pKa = 9.95MKK92 pKa = 10.54DD93 pKa = 3.3NNPLKK98 pKa = 10.19TGLFCNTYY106 pKa = 10.13PSTRR110 pKa = 11.84NQLIFQDD117 pKa = 4.07TTPCDD122 pKa = 3.47DD123 pKa = 3.17TDD125 pKa = 4.08YY126 pKa = 10.84NIEE129 pKa = 4.2YY130 pKa = 10.37EE131 pKa = 4.54VIDD134 pKa = 3.66

Molecular weight: 15.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0EFF0|T0EFF0_PAES9 Putative membrane protein OS=Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) OX=1292036 GN=H477_5415 PE=4 SV=1
SS1 pKa = 7.21GINARR6 pKa = 11.84VLFLFLFVFSPHH18 pKa = 5.32GTCPLSVSWPYY29 pKa = 10.78FSLRR33 pKa = 11.84WSLPPASGCTLKK45 pKa = 10.83QPDD48 pKa = 3.34SKK50 pKa = 11.25ARR52 pKa = 11.84SRR54 pKa = 11.84SPEE57 pKa = 3.98RR58 pKa = 11.84CDD60 pKa = 3.28GLYY63 pKa = 9.68WPGTICGPWPRR74 pKa = 11.84SRR76 pKa = 11.84EE77 pKa = 3.95TWTVTCAPATRR88 pKa = 11.84PARR91 pKa = 11.84TPQLPYY97 pKa = 10.54VPRR100 pKa = 11.84HH101 pKa = 4.75KK102 pKa = 10.36SARR105 pKa = 11.84RR106 pKa = 11.84DD107 pKa = 3.32SAMGFSLFARR117 pKa = 11.84RR118 pKa = 11.84YY119 pKa = 7.45WGNHH123 pKa = 3.27GCFLFLRR130 pKa = 11.84LLICLNPAGSPAA142 pKa = 3.36

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5956

0

5956

904124

31

2364

151.8

17.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.11 ± 0.04

1.189 ± 0.016

5.667 ± 0.032

7.013 ± 0.043

4.328 ± 0.03

6.145 ± 0.035

1.343 ± 0.016

10.5 ± 0.046

9.43 ± 0.042

9.179 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.016 ± 0.019

6.69 ± 0.037

2.648 ± 0.02

2.337 ± 0.021

2.963 ± 0.025

6.389 ± 0.038

4.792 ± 0.026

6.444 ± 0.033

0.595 ± 0.01

4.224 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski