Drosophila sturtevanti sigmavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Sturtevanti sigmavirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140D8M6|A0A140D8M6_9RHAB PP3 OS=Drosophila sturtevanti sigmavirus OX=1802946 GN=X PE=4 SV=1
MM1 pKa = 7.62ANCKK5 pKa = 8.57PTFEE9 pKa = 4.87LSHH12 pKa = 6.3EE13 pKa = 4.68LDD15 pKa = 5.05HH16 pKa = 7.56IWATTRR22 pKa = 11.84KK23 pKa = 9.58AIKK26 pKa = 10.0ALPKK30 pKa = 10.3LDD32 pKa = 4.07TNLKK36 pKa = 9.96SDD38 pKa = 3.36QNYY41 pKa = 10.14GDD43 pKa = 3.63NHH45 pKa = 6.67PDD47 pKa = 3.06KK48 pKa = 9.4TTLQSKK54 pKa = 8.18ITQQNSSGEE63 pKa = 4.13SDD65 pKa = 3.11SWAVLAMEE73 pKa = 4.99EE74 pKa = 4.49PDD76 pKa = 3.64NKK78 pKa = 10.67NISAEE83 pKa = 4.11VEE85 pKa = 3.72PDD87 pKa = 2.92QWPDD91 pKa = 3.17FDD93 pKa = 6.66DD94 pKa = 5.66SDD96 pKa = 3.99DD97 pKa = 3.79TSQEE101 pKa = 4.02LEE103 pKa = 4.05EE104 pKa = 6.18DD105 pKa = 3.48EE106 pKa = 5.11LFPIFQMKK114 pKa = 9.25NDD116 pKa = 3.29KK117 pKa = 9.82RR118 pKa = 11.84AEE120 pKa = 4.1MFSVPEE126 pKa = 4.02VEE128 pKa = 5.6QMVKK132 pKa = 10.88DD133 pKa = 4.3LLYY136 pKa = 10.7QLNLSNHH143 pKa = 6.94DD144 pKa = 3.53YY145 pKa = 10.61SYY147 pKa = 10.7HH148 pKa = 6.61IEE150 pKa = 4.04GTIRR154 pKa = 11.84RR155 pKa = 11.84PVLHH159 pKa = 5.82VNKK162 pKa = 10.04RR163 pKa = 11.84LTSPTSNVQPSTSKK177 pKa = 10.83RR178 pKa = 11.84IQTPEE183 pKa = 3.99NKK185 pKa = 9.24TKK187 pKa = 9.68STPPKK192 pKa = 10.58AVDD195 pKa = 3.29QEE197 pKa = 4.46SFNAVDD203 pKa = 3.57HH204 pKa = 6.97RR205 pKa = 11.84YY206 pKa = 10.12NDD208 pKa = 3.92LMRR211 pKa = 11.84KK212 pKa = 9.55NYY214 pKa = 7.93TLNYY218 pKa = 8.46IDD220 pKa = 4.0STIKK224 pKa = 10.27SKK226 pKa = 11.26YY227 pKa = 9.73VINKK231 pKa = 7.48EE232 pKa = 3.81NPDD235 pKa = 3.43FKK237 pKa = 10.76KK238 pKa = 10.51SYY240 pKa = 9.73FVEE243 pKa = 4.67VYY245 pKa = 9.93GANGKK250 pKa = 9.59FPALDD255 pKa = 2.8IHH257 pKa = 7.27LIQNILTIQKK267 pKa = 9.44KK268 pKa = 4.5WHH270 pKa = 5.5QMLNLVDD277 pKa = 4.38FTPTYY282 pKa = 7.53TTVV285 pKa = 2.66

Molecular weight: 32.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140D8M7|A0A140D8M7_9RHAB Matrix protein OS=Drosophila sturtevanti sigmavirus OX=1802946 GN=M PE=4 SV=1
MM1 pKa = 7.36SSSHH5 pKa = 6.77RR6 pKa = 11.84KK7 pKa = 6.62TSTYY11 pKa = 10.59IMKK14 pKa = 8.91KK15 pKa = 5.12TTLIVVVLSVVQVSQLTNLTCRR37 pKa = 11.84CEE39 pKa = 4.72LSDD42 pKa = 5.0LTDD45 pKa = 3.32STNDD49 pKa = 3.07ILVANVTYY57 pKa = 10.33DD58 pKa = 3.58LPHH61 pKa = 6.87NDD63 pKa = 4.74PYY65 pKa = 11.42NISQSINVNYY75 pKa = 10.01WNGTEE80 pKa = 4.04LVKK83 pKa = 10.12TKK85 pKa = 10.02QHH87 pKa = 5.73LCVYY91 pKa = 8.85LTDD94 pKa = 3.81EE95 pKa = 4.52SGMTVYY101 pKa = 10.96SNPDD105 pKa = 3.19DD106 pKa = 3.53TTKK109 pKa = 10.82NVEE112 pKa = 4.11SVIRR116 pKa = 11.84EE117 pKa = 3.77IKK119 pKa = 8.65TNEE122 pKa = 4.08GVPHH126 pKa = 7.66DD127 pKa = 5.55DD128 pKa = 3.44LTQSEE133 pKa = 5.05LLCTMGMCRR142 pKa = 11.84DD143 pKa = 3.41RR144 pKa = 11.84KK145 pKa = 10.69GSFKK149 pKa = 10.32EE150 pKa = 3.72ANRR153 pKa = 11.84GIRR156 pKa = 11.84NLNSILTLKK165 pKa = 10.9VNLTKK170 pKa = 10.79AGVPNEE176 pKa = 4.03RR177 pKa = 11.84SLMDD181 pKa = 3.87KK182 pKa = 10.84VNQQLMTSNHH192 pKa = 5.96IPNMGHH198 pKa = 6.65SSLLAQDD205 pKa = 4.34YY206 pKa = 10.55LFSRR210 pKa = 11.84PDD212 pKa = 3.05EE213 pKa = 4.38RR214 pKa = 11.84MEE216 pKa = 6.49DD217 pKa = 2.96IMFPSGEE224 pKa = 3.86HH225 pKa = 5.1RR226 pKa = 11.84RR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 11.84SLEE232 pKa = 3.87SEE234 pKa = 3.87TSRR237 pKa = 11.84EE238 pKa = 3.36IRR240 pKa = 11.84AWRR243 pKa = 11.84IRR245 pKa = 11.84RR246 pKa = 11.84LIKK249 pKa = 10.45KK250 pKa = 9.63IVGRR254 pKa = 11.84LEE256 pKa = 3.99KK257 pKa = 11.14YY258 pKa = 9.69EE259 pKa = 4.14VMWLEE264 pKa = 4.18NYY266 pKa = 9.13TLFLVYY272 pKa = 10.46LRR274 pKa = 11.84QSPHH278 pKa = 6.46FDD280 pKa = 2.82SSACVLHH287 pKa = 6.57PLFSDD292 pKa = 4.31LLKK295 pKa = 10.76CYY297 pKa = 10.07SANLHH302 pKa = 5.5VMNNTKK308 pKa = 10.46HH309 pKa = 6.3GVDD312 pKa = 3.08AMKK315 pKa = 10.89YY316 pKa = 8.71FAEE319 pKa = 4.65CVHH322 pKa = 6.49IKK324 pKa = 10.31YY325 pKa = 9.24SHH327 pKa = 6.74KK328 pKa = 10.12MEE330 pKa = 4.01QFMKK334 pKa = 10.53NYY336 pKa = 9.69LRR338 pKa = 11.84RR339 pKa = 11.84DD340 pKa = 3.62LLSLL344 pKa = 3.83

Molecular weight: 39.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4082

224

2144

680.3

77.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.361 ± 0.492

1.886 ± 0.268

5.585 ± 0.348

5.537 ± 0.259

4.312 ± 0.392

5.855 ± 0.748

2.964 ± 0.198

6.786 ± 0.474

5.928 ± 0.555

10.51 ± 0.609

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.287

4.679 ± 0.538

5.341 ± 0.382

3.577 ± 0.218

5.169 ± 0.54

7.545 ± 0.588

5.781 ± 0.442

5.904 ± 0.433

1.788 ± 0.203

4.067 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski