Clostridium sp. USBA 49

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2435 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4W7J5|A0A1T4W7J5_9CLOT Transcription elongation factor GreA OS=Clostridium sp. USBA 49 OX=1881060 GN=greA PE=3 SV=1
MM1 pKa = 7.34NKK3 pKa = 10.51DD4 pKa = 3.6NILKK8 pKa = 10.45KK9 pKa = 10.67LEE11 pKa = 4.23EE12 pKa = 4.11YY13 pKa = 10.65NINDD17 pKa = 3.93INIIDD22 pKa = 4.07YY23 pKa = 10.59KK24 pKa = 10.75EE25 pKa = 4.14KK26 pKa = 10.87DD27 pKa = 3.47VLVVSFYY34 pKa = 11.42YY35 pKa = 10.82DD36 pKa = 3.35FDD38 pKa = 6.79DD39 pKa = 5.63DD40 pKa = 4.85VINAAKK46 pKa = 10.28SYY48 pKa = 11.5ANDD51 pKa = 3.35EE52 pKa = 4.49SEE54 pKa = 5.13DD55 pKa = 3.97EE56 pKa = 4.31EE57 pKa = 5.59EE58 pKa = 4.6GDD60 pKa = 2.64IWYY63 pKa = 10.01EE64 pKa = 3.92EE65 pKa = 4.05FFIPYY70 pKa = 9.88LNDD73 pKa = 3.21YY74 pKa = 10.7AIDD77 pKa = 3.72EE78 pKa = 4.32VGQYY82 pKa = 9.72IEE84 pKa = 5.14EE85 pKa = 4.08IMEE88 pKa = 4.21EE89 pKa = 4.12LDD91 pKa = 3.62IEE93 pKa = 4.4AQFVSYY99 pKa = 10.28EE100 pKa = 4.06IEE102 pKa = 4.09KK103 pKa = 10.77EE104 pKa = 3.98NYY106 pKa = 9.77DD107 pKa = 3.5YY108 pKa = 11.85SEE110 pKa = 4.17FLAVFFPKK118 pKa = 10.53NKK120 pKa = 10.13AITIEE125 pKa = 3.99EE126 pKa = 4.22VIEE129 pKa = 4.44NIEE132 pKa = 3.75II133 pKa = 3.86

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4WZX8|A0A1T4WZX8_9CLOT Carbohydrate ABC transporter membrane protein 2 CUT1 family OS=Clostridium sp. USBA 49 OX=1881060 GN=SAMN05428976_105126 PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 4.04KK9 pKa = 8.76WNKK12 pKa = 8.31EE13 pKa = 4.05PKK15 pKa = 9.94FSTRR19 pKa = 11.84AYY21 pKa = 7.71TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.07AVLRR35 pKa = 11.84KK36 pKa = 9.54FGVCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.95LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2435

0

2435

772217

29

2882

317.1

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 0.054

1.074 ± 0.017

5.382 ± 0.031

7.402 ± 0.056

4.474 ± 0.042

6.24 ± 0.054

1.303 ± 0.017

10.721 ± 0.066

9.67 ± 0.057

8.861 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.023

6.764 ± 0.048

2.925 ± 0.025

2.283 ± 0.022

3.148 ± 0.031

6.014 ± 0.038

4.745 ± 0.034

5.944 ± 0.041

0.732 ± 0.018

4.218 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski