Beihai sobemo-like virus 26

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE73|A0A1L3KE73_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 26 OX=1922698 PE=4 SV=1
MM1 pKa = 7.83WNPDD5 pKa = 3.3LEE7 pKa = 4.39ATEE10 pKa = 4.95LVCSSWADD18 pKa = 3.27VEE20 pKa = 4.27RR21 pKa = 11.84ASFFRR26 pKa = 11.84DD27 pKa = 5.11FIQPHH32 pKa = 5.75LTQDD36 pKa = 3.42NLNSVLLVVATASVLSFLQYY56 pKa = 11.39ASILFVRR63 pKa = 11.84RR64 pKa = 11.84CRR66 pKa = 11.84SARR69 pKa = 11.84SLFFEE74 pKa = 4.61SVQAGAPFYY83 pKa = 11.02SSGEE87 pKa = 4.09SPPCQVEE94 pKa = 3.94MRR96 pKa = 11.84KK97 pKa = 10.02GSRR100 pKa = 11.84LVGFGIRR107 pKa = 11.84VGDD110 pKa = 4.13WIVTPQHH117 pKa = 6.11VADD120 pKa = 4.4SSDD123 pKa = 3.71NVWGGTSMFTFEE135 pKa = 5.37NYY137 pKa = 7.76STLCTDD143 pKa = 3.68AVAIRR148 pKa = 11.84IPANWNDD155 pKa = 3.1VRR157 pKa = 11.84KK158 pKa = 10.05ASVGALCKK166 pKa = 10.47NLIVRR171 pKa = 11.84CVGPMGEE178 pKa = 4.22WSCGRR183 pKa = 11.84LAEE186 pKa = 4.57ADD188 pKa = 3.17LWGGLDD194 pKa = 3.62YY195 pKa = 11.12FGSTDD200 pKa = 3.22HH201 pKa = 6.58GFSGAAYY208 pKa = 9.58ASGNTVYY215 pKa = 11.03GMHH218 pKa = 7.03CGGSGPNKK226 pKa = 10.18PNFGWSLSFLSCFVDD241 pKa = 5.36DD242 pKa = 4.39LAEE245 pKa = 4.08EE246 pKa = 4.15QRR248 pKa = 11.84TPVYY252 pKa = 9.9EE253 pKa = 4.13EE254 pKa = 3.77PSVRR258 pKa = 11.84EE259 pKa = 3.94VTTQAGKK266 pKa = 10.68RR267 pKa = 11.84KK268 pKa = 9.24IKK270 pKa = 10.06KK271 pKa = 8.88DD272 pKa = 2.83QGRR275 pKa = 11.84FVRR278 pKa = 11.84YY279 pKa = 6.12TQEE282 pKa = 3.82PTPVSVGRR290 pKa = 11.84HH291 pKa = 4.2VDD293 pKa = 3.2SSIVDD298 pKa = 3.66DD299 pKa = 4.23VAANADD305 pKa = 3.38NWFKK309 pKa = 11.43DD310 pKa = 3.37LGYY313 pKa = 10.41EE314 pKa = 3.95AALVEE319 pKa = 4.8SITKK323 pKa = 10.32KK324 pKa = 10.81VMNEE328 pKa = 3.53LSGNGLGPLRR338 pKa = 11.84GASTVSAVASPTSKK352 pKa = 10.5SSEE355 pKa = 3.97EE356 pKa = 4.13KK357 pKa = 10.7APSPAADD364 pKa = 3.39AQLPSTAQQSSPSPQLRR381 pKa = 11.84QTSTVTTVPEE391 pKa = 4.09KK392 pKa = 10.4LSKK395 pKa = 10.47RR396 pKa = 11.84MKK398 pKa = 10.59KK399 pKa = 9.52EE400 pKa = 3.47FQAAQVEE407 pKa = 4.35QLAWLRR413 pKa = 11.84EE414 pKa = 3.97TLTQRR419 pKa = 11.84FGFPVAALDD428 pKa = 3.7TTGWDD433 pKa = 3.53LQRR436 pKa = 11.84LQSHH440 pKa = 5.63VFVLQALEE448 pKa = 3.92AQSRR452 pKa = 11.84GKK454 pKa = 10.34VLSCTPASCPATDD467 pKa = 3.9QSQQGASKK475 pKa = 10.59SEE477 pKa = 4.03PSTGRR482 pKa = 3.16

Molecular weight: 52.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE73|A0A1L3KE73_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 26 OX=1922698 PE=4 SV=1
MM1 pKa = 7.35GLAAAAKK8 pKa = 10.04SRR10 pKa = 11.84FRR12 pKa = 11.84APGVGGSVEE21 pKa = 4.17RR22 pKa = 11.84KK23 pKa = 9.67SLEE26 pKa = 3.75LHH28 pKa = 6.55ASLMSCDD35 pKa = 3.34RR36 pKa = 11.84PVPARR41 pKa = 11.84SLQEE45 pKa = 4.0RR46 pKa = 11.84AFNWALRR53 pKa = 11.84EE54 pKa = 3.87LAPVCCEE61 pKa = 4.03LPPDD65 pKa = 3.99FFEE68 pKa = 5.21RR69 pKa = 11.84SHH71 pKa = 7.19FDD73 pKa = 3.11RR74 pKa = 11.84VLNSLNMQASPGYY87 pKa = 8.97PLMRR91 pKa = 11.84VAPTVGSYY99 pKa = 10.65LKK101 pKa = 10.64RR102 pKa = 11.84PDD104 pKa = 3.52GSWRR108 pKa = 11.84QDD110 pKa = 2.81RR111 pKa = 11.84VEE113 pKa = 3.98QLWASVLQRR122 pKa = 11.84VDD124 pKa = 3.44SDD126 pKa = 3.84PDD128 pKa = 4.14LIRR131 pKa = 11.84VFVKK135 pKa = 10.11QEE137 pKa = 3.4PHH139 pKa = 6.79RR140 pKa = 11.84IAKK143 pKa = 9.16VRR145 pKa = 11.84EE146 pKa = 3.58GRR148 pKa = 11.84MRR150 pKa = 11.84LIQAVSLVDD159 pKa = 3.37QVVDD163 pKa = 4.02HH164 pKa = 6.92LLHH167 pKa = 7.18DD168 pKa = 4.34HH169 pKa = 6.33LQQRR173 pKa = 11.84EE174 pKa = 3.85IEE176 pKa = 4.4LFGKK180 pKa = 9.62VPVMTGWAPVHH191 pKa = 5.93GGWRR195 pKa = 11.84HH196 pKa = 5.87LHH198 pKa = 6.01ADD200 pKa = 2.26WWGYY204 pKa = 10.45DD205 pKa = 3.12RR206 pKa = 11.84SAWDD210 pKa = 3.11WTVPAWLLRR219 pKa = 11.84LEE221 pKa = 3.99WRR223 pKa = 11.84LRR225 pKa = 11.84EE226 pKa = 4.08ALTINPTRR234 pKa = 11.84LWLSQMQKK242 pKa = 10.34RR243 pKa = 11.84YY244 pKa = 10.72DD245 pKa = 3.66EE246 pKa = 5.61LFVCPEE252 pKa = 4.23FVLSDD257 pKa = 3.4GMVVKK262 pKa = 10.17QCIEE266 pKa = 3.97GVMKK270 pKa = 10.2TGSVVTASANSHH282 pKa = 5.11MQLLLHH288 pKa = 6.73AVAAFAAGEE297 pKa = 4.24EE298 pKa = 4.26PGPLIAMGDD307 pKa = 3.76DD308 pKa = 3.35TMQPLGSEE316 pKa = 4.69AYY318 pKa = 9.84LQEE321 pKa = 3.42LRR323 pKa = 11.84KK324 pKa = 9.44YY325 pKa = 10.44VNVKK329 pKa = 10.02EE330 pKa = 4.29PEE332 pKa = 4.06LGEE335 pKa = 3.95FCSRR339 pKa = 11.84VIQGSRR345 pKa = 11.84VEE347 pKa = 4.03PQNILKK353 pKa = 10.26HH354 pKa = 4.73VANLITAPPEE364 pKa = 3.99FLDD367 pKa = 5.85DD368 pKa = 3.98MLLSLQVEE376 pKa = 4.7YY377 pKa = 10.99ARR379 pKa = 11.84SRR381 pKa = 11.84WLRR384 pKa = 11.84LWQLTARR391 pKa = 11.84RR392 pKa = 11.84LGCRR396 pKa = 11.84VRR398 pKa = 11.84PQIWLLDD405 pKa = 3.97VYY407 pKa = 11.46DD408 pKa = 4.03EE409 pKa = 4.37AA410 pKa = 6.63

Molecular weight: 46.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

892

410

482

446.0

49.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.857 ± 0.345

2.018 ± 0.19

5.157 ± 0.128

5.942 ± 0.394

3.812 ± 0.541

6.614 ± 0.614

2.018 ± 0.556

2.354 ± 0.201

3.475 ± 0.335

9.865 ± 1.574

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.568

2.578 ± 0.384

5.717 ± 0.232

5.269 ± 0.09

7.175 ± 1.131

8.857 ± 1.687

4.484 ± 1.251

8.744 ± 0.171

2.915 ± 0.455

1.906 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski