Amino acid dipepetide frequency for Neorhizobium sp. SOG26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.462AlaAla: 15.462 ± 0.149
0.946AlaCys: 0.946 ± 0.026
6.389AlaAsp: 6.389 ± 0.071
7.671AlaGlu: 7.671 ± 0.093
4.48AlaPhe: 4.48 ± 0.055
9.665AlaGly: 9.665 ± 0.101
2.007AlaHis: 2.007 ± 0.04
6.574AlaIle: 6.574 ± 0.073
4.529AlaLys: 4.529 ± 0.065
11.971AlaLeu: 11.971 ± 0.115
3.615AlaMet: 3.615 ± 0.052
2.959AlaAsn: 2.959 ± 0.051
4.859AlaPro: 4.859 ± 0.073
3.674AlaGln: 3.674 ± 0.053
7.393AlaArg: 7.393 ± 0.085
6.578AlaSer: 6.578 ± 0.071
5.624AlaThr: 5.624 ± 0.075
8.558AlaVal: 8.558 ± 0.089
1.325AlaTrp: 1.325 ± 0.032
2.572AlaTyr: 2.572 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.828CysAla: 0.828 ± 0.027
0.094CysCys: 0.094 ± 0.009
0.459CysAsp: 0.459 ± 0.02
0.403CysGlu: 0.403 ± 0.018
0.319CysPhe: 0.319 ± 0.016
0.876CysGly: 0.876 ± 0.026
0.209CysHis: 0.209 ± 0.014
0.406CysIle: 0.406 ± 0.018
0.2CysLys: 0.2 ± 0.012
0.785CysLeu: 0.785 ± 0.025
0.171CysMet: 0.171 ± 0.01
0.205CysAsn: 0.205 ± 0.012
0.348CysPro: 0.348 ± 0.017
0.233CysGln: 0.233 ± 0.013
0.557CysArg: 0.557 ± 0.021
0.461CysSer: 0.461 ± 0.017
0.39CysThr: 0.39 ± 0.017
0.584CysVal: 0.584 ± 0.022
0.09CysTrp: 0.09 ± 0.008
0.191CysTyr: 0.191 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.31AspAla: 6.31 ± 0.078
0.438AspCys: 0.438 ± 0.022
2.968AspAsp: 2.968 ± 0.053
3.663AspGlu: 3.663 ± 0.062
2.206AspPhe: 2.206 ± 0.048
4.782AspGly: 4.782 ± 0.068
1.205AspHis: 1.205 ± 0.033
3.228AspIle: 3.228 ± 0.055
1.984AspLys: 1.984 ± 0.045
5.838AspLeu: 5.838 ± 0.068
1.453AspMet: 1.453 ± 0.036
1.466AspAsn: 1.466 ± 0.032
3.267AspPro: 3.267 ± 0.058
1.749AspGln: 1.749 ± 0.035
3.988AspArg: 3.988 ± 0.058
2.046AspSer: 2.046 ± 0.038
2.424AspThr: 2.424 ± 0.046
4.182AspVal: 4.182 ± 0.056
0.926AspTrp: 0.926 ± 0.027
1.509AspTyr: 1.509 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
7.786GluAla: 7.786 ± 0.085
0.337GluCys: 0.337 ± 0.015
3.09GluAsp: 3.09 ± 0.054
3.898GluGlu: 3.898 ± 0.07
1.938GluPhe: 1.938 ± 0.036
4.281GluGly: 4.281 ± 0.067
1.241GluHis: 1.241 ± 0.031
3.835GluIle: 3.835 ± 0.055
2.956GluLys: 2.956 ± 0.05
5.589GluLeu: 5.589 ± 0.069
1.658GluMet: 1.658 ± 0.037
1.91GluAsn: 1.91 ± 0.041
2.836GluPro: 2.836 ± 0.062
2.325GluGln: 2.325 ± 0.047
4.734GluArg: 4.734 ± 0.069
2.377GluSer: 2.377 ± 0.039
3.618GluThr: 3.618 ± 0.059
4.122GluVal: 4.122 ± 0.057
0.716GluTrp: 0.716 ± 0.022
1.11GluTyr: 1.11 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.476PheAla: 4.476 ± 0.059
0.381PheCys: 0.381 ± 0.015
2.695PheAsp: 2.695 ± 0.049
2.24PheGlu: 2.24 ± 0.044
1.614PhePhe: 1.614 ± 0.039
3.836PheGly: 3.836 ± 0.06
0.772PheHis: 0.772 ± 0.027
1.995PheIle: 1.995 ± 0.043
1.223PheLys: 1.223 ± 0.028
3.643PheLeu: 3.643 ± 0.059
0.918PheMet: 0.918 ± 0.025
1.186PheAsn: 1.186 ± 0.032
1.645PhePro: 1.645 ± 0.036
1.117PheGln: 1.117 ± 0.028
2.295PheArg: 2.295 ± 0.044
2.532PheSer: 2.532 ± 0.042
2.02PheThr: 2.02 ± 0.045
2.824PheVal: 2.824 ± 0.049
0.612PheTrp: 0.612 ± 0.024
0.946PheTyr: 0.946 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
8.242GlyAla: 8.242 ± 0.084
0.766GlyCys: 0.766 ± 0.026
4.162GlyAsp: 4.162 ± 0.07
4.796GlyGlu: 4.796 ± 0.065
3.676GlyPhe: 3.676 ± 0.063
6.821GlyGly: 6.821 ± 0.106
1.734GlyHis: 1.734 ± 0.038
4.958GlyIle: 4.958 ± 0.068
3.711GlyLys: 3.711 ± 0.063
8.319GlyLeu: 8.319 ± 0.087
2.42GlyMet: 2.42 ± 0.043
2.331GlyAsn: 2.331 ± 0.047
3.126GlyPro: 3.126 ± 0.051
2.757GlyGln: 2.757 ± 0.049
5.552GlyArg: 5.552 ± 0.066
4.814GlySer: 4.814 ± 0.062
4.503GlyThr: 4.503 ± 0.064
5.853GlyVal: 5.853 ± 0.068
1.218GlyTrp: 1.218 ± 0.033
2.371GlyTyr: 2.371 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.042HisAla: 2.042 ± 0.041
0.205HisCys: 0.205 ± 0.012
1.225HisAsp: 1.225 ± 0.034
1.139HisGlu: 1.139 ± 0.031
0.878HisPhe: 0.878 ± 0.026
1.866HisGly: 1.866 ± 0.034
0.564HisHis: 0.564 ± 0.023
0.962HisIle: 0.962 ± 0.028
0.55HisLys: 0.55 ± 0.018
2.035HisLeu: 2.035 ± 0.041
0.567HisMet: 0.567 ± 0.019
0.476HisAsn: 0.476 ± 0.02
1.283HisPro: 1.283 ± 0.03
0.661HisGln: 0.661 ± 0.021
1.283HisArg: 1.283 ± 0.031
1.02HisSer: 1.02 ± 0.027
0.785HisThr: 0.785 ± 0.023
1.571HisVal: 1.571 ± 0.032
0.291HisTrp: 0.291 ± 0.014
0.542HisTyr: 0.542 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.203IleAla: 7.203 ± 0.071
0.563IleCys: 0.563 ± 0.02
3.573IleAsp: 3.573 ± 0.057
3.786IleGlu: 3.786 ± 0.049
2.08IlePhe: 2.08 ± 0.042
5.205IleGly: 5.205 ± 0.067
0.978IleHis: 0.978 ± 0.027
2.933IleIle: 2.933 ± 0.054
1.774IleLys: 1.774 ± 0.04
4.921IleLeu: 4.921 ± 0.068
1.229IleMet: 1.229 ± 0.028
1.698IleAsn: 1.698 ± 0.034
2.468IlePro: 2.468 ± 0.049
1.387IleGln: 1.387 ± 0.033
3.526IleArg: 3.526 ± 0.054
3.458IleSer: 3.458 ± 0.054
2.979IleThr: 2.979 ± 0.047
4.382IleVal: 4.382 ± 0.063
0.667IleTrp: 0.667 ± 0.025
1.32IleTyr: 1.32 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.799LysAla: 4.799 ± 0.066
0.163LysCys: 0.163 ± 0.012
2.114LysAsp: 2.114 ± 0.042
2.058LysGlu: 2.058 ± 0.039
1.041LysPhe: 1.041 ± 0.032
2.838LysGly: 2.838 ± 0.059
0.731LysHis: 0.731 ± 0.021
2.172LysIle: 2.172 ± 0.041
1.679LysLys: 1.679 ± 0.044
3.991LysLeu: 3.991 ± 0.059
1.002LysMet: 1.002 ± 0.03
1.175LysAsn: 1.175 ± 0.033
2.413LysPro: 2.413 ± 0.05
1.244LysGln: 1.244 ± 0.032
2.691LysArg: 2.691 ± 0.047
2.144LysSer: 2.144 ± 0.039
2.311LysThr: 2.311 ± 0.047
2.863LysVal: 2.863 ± 0.052
0.4LysTrp: 0.4 ± 0.018
0.683LysTyr: 0.683 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
12.365LeuAla: 12.365 ± 0.115
0.813LeuCys: 0.813 ± 0.024
5.74LeuAsp: 5.74 ± 0.063
5.567LeuGlu: 5.567 ± 0.079
3.715LeuPhe: 3.715 ± 0.059
7.972LeuGly: 7.972 ± 0.081
1.733LeuHis: 1.733 ± 0.036
5.268LeuIle: 5.268 ± 0.073
4.281LeuLys: 4.281 ± 0.058
9.449LeuLeu: 9.449 ± 0.11
2.459LeuMet: 2.459 ± 0.047
2.752LeuAsn: 2.752 ± 0.047
5.29LeuPro: 5.29 ± 0.069
2.903LeuGln: 2.903 ± 0.059
6.031LeuArg: 6.031 ± 0.079
6.858LeuSer: 6.858 ± 0.072
5.514LeuThr: 5.514 ± 0.073
7.522LeuVal: 7.522 ± 0.087
1.077LeuTrp: 1.077 ± 0.029
2.085LeuTyr: 2.085 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
3.193MetAla: 3.193 ± 0.056
0.132MetCys: 0.132 ± 0.01
1.243MetAsp: 1.243 ± 0.03
1.341MetGlu: 1.341 ± 0.031
0.824MetPhe: 0.824 ± 0.026
1.827MetGly: 1.827 ± 0.044
0.461MetHis: 0.461 ± 0.019
1.642MetIle: 1.642 ± 0.04
1.205MetLys: 1.205 ± 0.029
2.667MetLeu: 2.667 ± 0.047
0.789MetMet: 0.789 ± 0.029
0.944MetAsn: 0.944 ± 0.027
1.608MetPro: 1.608 ± 0.036
0.954MetGln: 0.954 ± 0.026
1.848MetArg: 1.848 ± 0.04
1.805MetSer: 1.805 ± 0.038
1.981MetThr: 1.981 ± 0.037
1.803MetVal: 1.803 ± 0.033
0.209MetTrp: 0.209 ± 0.011
0.33MetTyr: 0.33 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.25AsnAla: 3.25 ± 0.059
0.244AsnCys: 0.244 ± 0.015
1.475AsnAsp: 1.475 ± 0.032
1.495AsnGlu: 1.495 ± 0.031
1.095AsnPhe: 1.095 ± 0.033
2.601AsnGly: 2.601 ± 0.045
0.61AsnHis: 0.61 ± 0.022
1.613AsnIle: 1.613 ± 0.036
0.923AsnLys: 0.923 ± 0.025
2.861AsnLeu: 2.861 ± 0.045
0.744AsnMet: 0.744 ± 0.025
0.901AsnAsn: 0.901 ± 0.028
1.938AsnPro: 1.938 ± 0.036
0.895AsnGln: 0.895 ± 0.022
2.019AsnArg: 2.019 ± 0.037
1.462AsnSer: 1.462 ± 0.037
1.364AsnThr: 1.364 ± 0.034
2.058AsnVal: 2.058 ± 0.04
0.476AsnTrp: 0.476 ± 0.02
0.704AsnTyr: 0.704 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
5.704ProAla: 5.704 ± 0.089
0.267ProCys: 0.267 ± 0.012
3.351ProAsp: 3.351 ± 0.054
3.717ProGlu: 3.717 ± 0.055
1.998ProPhe: 1.998 ± 0.04
3.946ProGly: 3.946 ± 0.06
1.071ProHis: 1.071 ± 0.028
2.406ProIle: 2.406 ± 0.049
1.934ProLys: 1.934 ± 0.044
4.516ProLeu: 4.516 ± 0.062
1.211ProMet: 1.211 ± 0.031
1.509ProAsn: 1.509 ± 0.036
2.26ProPro: 2.26 ± 0.057
1.759ProGln: 1.759 ± 0.035
2.625ProArg: 2.625 ± 0.043
2.796ProSer: 2.796 ± 0.047
2.427ProThr: 2.427 ± 0.047
4.197ProVal: 4.197 ± 0.058
0.623ProTrp: 0.623 ± 0.022
1.222ProTyr: 1.222 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.085GlnAla: 4.085 ± 0.054
0.199GlnCys: 0.199 ± 0.013
1.555GlnAsp: 1.555 ± 0.034
1.773GlnGlu: 1.773 ± 0.041
1.139GlnPhe: 1.139 ± 0.027
2.269GlnGly: 2.269 ± 0.043
0.666GlnHis: 0.666 ± 0.021
1.901GlnIle: 1.901 ± 0.032
1.323GlnLys: 1.323 ± 0.037
2.974GlnLeu: 2.974 ± 0.053
1.006GlnMet: 1.006 ± 0.03
0.95GlnAsn: 0.95 ± 0.028
1.802GlnPro: 1.802 ± 0.04
1.342GlnGln: 1.342 ± 0.044
2.251GlnArg: 2.251 ± 0.048
1.898GlnSer: 1.898 ± 0.039
1.77GlnThr: 1.77 ± 0.037
2.338GlnVal: 2.338 ± 0.046
0.383GlnTrp: 0.383 ± 0.015
0.657GlnTyr: 0.657 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
6.581ArgAla: 6.581 ± 0.067
0.419ArgCys: 0.419 ± 0.019
3.703ArgAsp: 3.703 ± 0.062
4.219ArgGlu: 4.219 ± 0.068
2.801ArgPhe: 2.801 ± 0.046
4.376ArgGly: 4.376 ± 0.057
1.625ArgHis: 1.625 ± 0.037
3.889ArgIle: 3.889 ± 0.057
2.55ArgLys: 2.55 ± 0.048
7.142ArgLeu: 7.142 ± 0.092
1.917ArgMet: 1.917 ± 0.042
2.081ArgAsn: 2.081 ± 0.038
3.097ArgPro: 3.097 ± 0.046
2.685ArgGln: 2.685 ± 0.049
5.074ArgArg: 5.074 ± 0.077
3.759ArgSer: 3.759 ± 0.058
3.147ArgThr: 3.147 ± 0.046
4.317ArgVal: 4.317 ± 0.057
0.865ArgTrp: 0.865 ± 0.026
1.788ArgTyr: 1.788 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.246SerAla: 6.246 ± 0.077
0.426SerCys: 0.426 ± 0.019
3.056SerAsp: 3.056 ± 0.047
3.14SerGlu: 3.14 ± 0.053
2.546SerPhe: 2.546 ± 0.041
5.668SerGly: 5.668 ± 0.068
1.183SerHis: 1.183 ± 0.028
3.138SerIle: 3.138 ± 0.052
1.949SerLys: 1.949 ± 0.036
5.786SerLeu: 5.786 ± 0.074
1.482SerMet: 1.482 ± 0.033
1.597SerAsn: 1.597 ± 0.035
2.814SerPro: 2.814 ± 0.043
1.759SerGln: 1.759 ± 0.031
3.838SerArg: 3.838 ± 0.058
3.472SerSer: 3.472 ± 0.056
2.9SerThr: 2.9 ± 0.05
4.267SerVal: 4.267 ± 0.054
0.719SerTrp: 0.719 ± 0.025
1.424SerTyr: 1.424 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
6.007ThrAla: 6.007 ± 0.066
0.42ThrCys: 0.42 ± 0.017
2.768ThrAsp: 2.768 ± 0.048
2.867ThrGlu: 2.867 ± 0.048
2.153ThrPhe: 2.153 ± 0.038
4.958ThrGly: 4.958 ± 0.073
0.976ThrHis: 0.976 ± 0.024
3.105ThrIle: 3.105 ± 0.044
1.753ThrLys: 1.753 ± 0.041
5.482ThrLeu: 5.482 ± 0.073
1.3ThrMet: 1.3 ± 0.031
1.445ThrAsn: 1.445 ± 0.039
2.986ThrPro: 2.986 ± 0.049
1.462ThrGln: 1.462 ± 0.035
3.075ThrArg: 3.075 ± 0.047
3.076ThrSer: 3.076 ± 0.045
2.843ThrThr: 2.843 ± 0.049
4.483ThrVal: 4.483 ± 0.067
0.669ThrTrp: 0.669 ± 0.021
1.249ThrTyr: 1.249 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.762ValAla: 8.762 ± 0.083
0.642ValCys: 0.642 ± 0.021
4.012ValAsp: 4.012 ± 0.055
4.712ValGlu: 4.712 ± 0.056
2.899ValPhe: 2.899 ± 0.05
5.445ValGly: 5.445 ± 0.063
1.387ValHis: 1.387 ± 0.031
4.332ValIle: 4.332 ± 0.065
2.674ValLys: 2.674 ± 0.047
7.452ValLeu: 7.452 ± 0.087
2.001ValMet: 2.001 ± 0.039
2.046ValAsn: 2.046 ± 0.041
3.681ValPro: 3.681 ± 0.054
2.078ValGln: 2.078 ± 0.043
4.533ValArg: 4.533 ± 0.058
4.762ValSer: 4.762 ± 0.053
4.591ValThr: 4.591 ± 0.067
6.035ValVal: 6.035 ± 0.074
0.848ValTrp: 0.848 ± 0.025
1.548ValTyr: 1.548 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.105TrpAla: 1.105 ± 0.031
0.131TrpCys: 0.131 ± 0.01
0.606TrpAsp: 0.606 ± 0.02
0.564TrpGlu: 0.564 ± 0.019
0.585TrpPhe: 0.585 ± 0.022
0.778TrpGly: 0.778 ± 0.027
0.298TrpHis: 0.298 ± 0.014
0.684TrpIle: 0.684 ± 0.023
0.57TrpLys: 0.57 ± 0.023
1.58TrpLeu: 1.58 ± 0.042
0.369TrpMet: 0.369 ± 0.016
0.451TrpAsn: 0.451 ± 0.02
0.661TrpPro: 0.661 ± 0.023
0.526TrpGln: 0.526 ± 0.019
0.995TrpArg: 0.995 ± 0.028
0.811TrpSer: 0.811 ± 0.025
0.727TrpThr: 0.727 ± 0.023
0.751TrpVal: 0.751 ± 0.024
0.228TrpTrp: 0.228 ± 0.014
0.307TrpTyr: 0.307 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.402TyrAla: 2.402 ± 0.048
0.221TyrCys: 0.221 ± 0.012
1.444TyrAsp: 1.444 ± 0.033
1.309TyrGlu: 1.309 ± 0.032
0.984TyrPhe: 0.984 ± 0.026
2.12TyrGly: 2.12 ± 0.039
0.496TyrHis: 0.496 ± 0.02
1.089TyrIle: 1.089 ± 0.028
0.745TyrLys: 0.745 ± 0.027
2.393TyrLeu: 2.393 ± 0.044
0.491TyrMet: 0.491 ± 0.02
0.646TyrAsn: 0.646 ± 0.021
1.16TyrPro: 1.16 ± 0.032
0.774TyrGln: 0.774 ± 0.021
1.727TyrArg: 1.727 ± 0.032
1.28TyrSer: 1.28 ± 0.034
1.158TyrThr: 1.158 ± 0.031
1.73TyrVal: 1.73 ± 0.039
0.385TyrTrp: 0.385 ± 0.017
0.677TyrTyr: 0.677 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4290 proteins (1337437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski