Puccinia graminis f. sp. tritici

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia; Puccinia graminis

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B0P5Q9|A0A5B0P5Q9_PUCGR Uncharacterized protein OS=Puccinia graminis f. sp. tritici OX=56615 GN=PGT21_012049 PE=4 SV=1
MM1 pKa = 6.93QSDD4 pKa = 3.98MGRR7 pKa = 11.84LINSFNLLMSDD18 pKa = 5.0CMQSDD23 pKa = 3.97TGRR26 pKa = 11.84LIDD29 pKa = 4.0SFNLAMSDD37 pKa = 3.85CMQSDD42 pKa = 3.7TSGLINSFNWPVSDD56 pKa = 4.69YY57 pKa = 9.05MQSDD61 pKa = 3.88MGRR64 pKa = 11.84LINSFNLPVSDD75 pKa = 5.07CMQSDD80 pKa = 4.07MSRR83 pKa = 11.84LIDD86 pKa = 3.95SFNLPMSDD94 pKa = 4.35CMQSDD99 pKa = 3.7TSGLINSFNWPVSDD113 pKa = 4.69YY114 pKa = 9.05MQSDD118 pKa = 3.88MGRR121 pKa = 11.84LINSFNLLMSDD132 pKa = 4.74CMQSDD137 pKa = 3.9TSGMINSFNWPVSDD151 pKa = 4.69YY152 pKa = 9.39MQSDD156 pKa = 3.39MGKK159 pKa = 9.9LKK161 pKa = 10.96LSINPLVSDD170 pKa = 4.94CMQSDD175 pKa = 3.88MGQLINSFNN184 pKa = 3.53

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B0NLS9|A0A5B0NLS9_PUCGR Uncharacterized protein OS=Puccinia graminis f. sp. tritici OX=56615 GN=PGT21_016218 PE=4 SV=1
MM1 pKa = 7.28TRR3 pKa = 11.84LKK5 pKa = 10.96VGSNWAARR13 pKa = 11.84RR14 pKa = 11.84AGAVGSLGAGPAWSVFSIPNARR36 pKa = 11.84GQPGYY41 pKa = 11.11NRR43 pKa = 11.84GQPAKK48 pKa = 9.33TRR50 pKa = 11.84AARR53 pKa = 11.84GLPAGSLNRR62 pKa = 11.84KK63 pKa = 7.58RR64 pKa = 11.84VRR66 pKa = 11.84VGLPISS72 pKa = 3.6

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34829

1519

36348

13159220

47

4472

362.0

40.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.935 ± 0.013

1.334 ± 0.006

5.33 ± 0.01

5.791 ± 0.013

3.64 ± 0.008

5.627 ± 0.015

2.95 ± 0.009

5.186 ± 0.01

5.424 ± 0.014

9.395 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.932 ± 0.005

4.515 ± 0.009

6.913 ± 0.018

4.794 ± 0.01

5.666 ± 0.009

10.02 ± 0.02

5.995 ± 0.009

5.011 ± 0.01

1.185 ± 0.004

2.356 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski