Pantoea cypripedii (Pectobacterium cypripedii) (Erwinia cypripedii)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1EY03|A0A1X1EY03_PANCY ATP-binding protein OS=Pantoea cypripedii OX=55209 GN=HA50_16835 PE=4 SV=1
MM1 pKa = 7.64LGTVTAGSNGAWSFTTSTLADD22 pKa = 3.54GTHH25 pKa = 6.16TLNVTATDD33 pKa = 3.4AAGNVSPNASVTLTVDD49 pKa = 3.21TGVPTASTLTVTADD63 pKa = 3.1NVTPNVTIPSGGYY76 pKa = 8.76TNDD79 pKa = 3.3NTPTLSGTAEE89 pKa = 4.0AGAVVTISDD98 pKa = 3.9GSTVLGSVTAGAGGAWSFTTSALSNGTHH126 pKa = 6.82PLSVTVKK133 pKa = 9.97DD134 pKa = 3.39AAGNVSPATAATVIVDD150 pKa = 4.11TVAPTASTLTITADD164 pKa = 3.12NVTPNVTVPSGGSTNDD180 pKa = 3.29TTPTLSGTAEE190 pKa = 4.0IGSKK194 pKa = 7.88VTILDD199 pKa = 3.58GSTVLGTLTVGGSGTWSFTTATLSNGTHH227 pKa = 6.75PLSVTVTDD235 pKa = 4.04LAGNVSGTTSASVIIDD251 pKa = 3.71TVAPAAVTGLAASNNNGGSAVAIPNNGTTNDD282 pKa = 3.75NTPLLSGTAEE292 pKa = 4.01AGAKK296 pKa = 9.69ISIYY300 pKa = 10.87DD301 pKa = 3.58GATLLGTTTAASSGAWSFTTSTLTEE326 pKa = 4.22GTHH329 pKa = 5.25TLNVTATDD337 pKa = 3.4AAGNVSPNASVTLTVDD353 pKa = 3.5SVAPTNSTLTVTNDD367 pKa = 2.97SVTPNVTVPSGGYY380 pKa = 8.02TRR382 pKa = 11.84DD383 pKa = 3.38TTPVLTGTAEE393 pKa = 3.79VGSRR397 pKa = 11.84VTIYY401 pKa = 10.93DD402 pKa = 3.33GATILGSVTVGAGGTWSFTTAVLANGSHH430 pKa = 6.92PLSVTVTDD438 pKa = 3.57AAGNVSGITSSTVNVDD454 pKa = 3.42TVAPTAATALAVNTAGTTLTGTGEE478 pKa = 4.3AGTTVTVKK486 pKa = 10.73DD487 pKa = 3.75AGGNTIGTGTVGVTGSFSVTLTTAQTTGATLSVSLTDD524 pKa = 3.63AAGNTSPTSTVLGAIKK540 pKa = 10.02IVAVNDD546 pKa = 3.93LNEE549 pKa = 4.61LDD551 pKa = 4.01YY552 pKa = 10.92STTSGTITNTTGTTNTGTALLSVNLGSVLGVGVLSSSNAYY592 pKa = 8.25MFNVGTGDD600 pKa = 3.28TRR602 pKa = 11.84TVTLHH607 pKa = 6.73ASEE610 pKa = 4.69TSLISLIGSYY620 pKa = 10.5SLYY623 pKa = 10.47LYY625 pKa = 9.2QQQTDD630 pKa = 4.17GTWKK634 pKa = 10.68VISTTTNYY642 pKa = 10.01ISTTLLSLLTQTGSNITYY660 pKa = 10.69SNLGTGNYY668 pKa = 10.02AVVLGASSGISVLPSTSVTTVSDD691 pKa = 3.1ITTIAATVAATVTGNLLTNDD711 pKa = 3.65TSSVAGTVPTGTAVTSVSGHH731 pKa = 4.76TVGTSTSFSTAYY743 pKa = 8.38GTLTIDD749 pKa = 2.94SHH751 pKa = 7.59GNYY754 pKa = 9.57TYY756 pKa = 10.06TLKK759 pKa = 10.98AGLDD763 pKa = 3.47AGTLPVADD771 pKa = 3.86TFSYY775 pKa = 11.02SVTDD779 pKa = 3.57AQGVVTTATLTIDD792 pKa = 4.09LVHH795 pKa = 6.33GTTTASLLSVNSLFAEE811 pKa = 4.41ATTTTDD817 pKa = 2.73SDD819 pKa = 3.95HH820 pKa = 6.77SVSGSIWADD829 pKa = 2.66SSTSHH834 pKa = 6.68TGTLTITNEE843 pKa = 4.38HH844 pKa = 6.82GDD846 pKa = 3.74STT848 pKa = 4.46

Molecular weight: 82.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1EQF3|A0A1X1EQF3_PANCY Proton/glutamate-aspartate symporter OS=Pantoea cypripedii OX=55209 GN=gltP PE=3 SV=1
MM1 pKa = 7.57EE2 pKa = 5.42MGDD5 pKa = 3.67FSPRR9 pKa = 11.84QQVSPAVRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84AQQLFRR25 pKa = 11.84AACCGQKK32 pKa = 9.99VFSRR36 pKa = 11.84LTMNGYY42 pKa = 10.41LKK44 pKa = 10.15IDD46 pKa = 3.45VGPFWRR52 pKa = 11.84ILSKK56 pKa = 11.11DD57 pKa = 3.41GGRR60 pKa = 11.84QWWLMDD66 pKa = 3.11HH67 pKa = 5.66EE68 pKa = 4.92TYY70 pKa = 10.66NRR72 pKa = 11.84EE73 pKa = 3.48IRR75 pKa = 11.84RR76 pKa = 3.75

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5848

0

5848

1873892

29

3919

320.4

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.238 ± 0.043

1.001 ± 0.011

5.202 ± 0.026

5.297 ± 0.032

3.815 ± 0.021

7.419 ± 0.036

2.329 ± 0.016

5.575 ± 0.028

3.78 ± 0.03

11.118 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.017

3.603 ± 0.025

4.591 ± 0.022

4.92 ± 0.035

5.791 ± 0.033

6.119 ± 0.027

5.336 ± 0.036

6.954 ± 0.027

1.55 ± 0.013

2.67 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski