Formosa sp. Hel1_33_131

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Formosa; unclassified Formosa

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7XVK9|A0A1D7XVK9_9FLAO Por_Secre_tail domain-containing protein OS=Formosa sp. Hel1_33_131 OX=1336794 GN=FORMB_03070 PE=4 SV=1
MM1 pKa = 7.58SAQDD5 pKa = 3.27SKK7 pKa = 11.65GYY9 pKa = 10.23VGVSIGAAIPQGDD22 pKa = 4.12LADD25 pKa = 3.88TAKK28 pKa = 10.26TGLDD32 pKa = 3.41LGLINAGYY40 pKa = 10.15RR41 pKa = 11.84FNEE44 pKa = 3.96TWGATVNWGATGHH57 pKa = 6.54ALEE60 pKa = 4.9GTDD63 pKa = 4.51DD64 pKa = 3.87GSIGVGYY71 pKa = 9.08FAVGPMISFGDD82 pKa = 3.85FDD84 pKa = 5.71FKK86 pKa = 10.77PQYY89 pKa = 10.33AFSSATIDD97 pKa = 3.35SGGAEE102 pKa = 4.63LDD104 pKa = 3.33VDD106 pKa = 4.83MEE108 pKa = 4.65SAWIIGATYY117 pKa = 10.42NYY119 pKa = 10.83SLGDD123 pKa = 3.58NWGLAANLDD132 pKa = 4.03YY133 pKa = 9.77FTFTVEE139 pKa = 4.69DD140 pKa = 4.19ADD142 pKa = 4.34DD143 pKa = 4.31SEE145 pKa = 6.41SIMKK149 pKa = 10.0FSVGVQYY156 pKa = 11.3KK157 pKa = 8.89FF158 pKa = 3.32

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7Y1F3|A0A1D7Y1F3_9FLAO Alpha/beta hydrolase OS=Formosa sp. Hel1_33_131 OX=1336794 GN=FORMB_23860 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.34KK41 pKa = 10.22ISVSSEE47 pKa = 3.61LRR49 pKa = 11.84HH50 pKa = 6.16KK51 pKa = 10.53KK52 pKa = 10.04

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2626

0

2626

824978

31

4886

314.2

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.426 ± 0.055

0.786 ± 0.02

5.635 ± 0.041

6.334 ± 0.053

5.229 ± 0.036

6.381 ± 0.051

1.773 ± 0.022

7.753 ± 0.04

7.371 ± 0.077

9.283 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.025

6.118 ± 0.057

3.413 ± 0.038

3.46 ± 0.028

3.222 ± 0.035

6.989 ± 0.051

6.469 ± 0.087

6.278 ± 0.042

1.001 ± 0.018

3.995 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski