Blueberry virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7MBT3|J7MBT3_9CLOS Coat protein homolog OS=Blueberry virus A OX=1206566 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.16FMRR5 pKa = 11.84RR6 pKa = 11.84LDD8 pKa = 3.94EE9 pKa = 4.21VHH11 pKa = 7.06CLATPCLHH19 pKa = 6.67LVDD22 pKa = 4.92NISRR26 pKa = 11.84IAYY29 pKa = 9.83SITLPSHH36 pKa = 7.13VYY38 pKa = 7.2QHH40 pKa = 6.62CASQLRR46 pKa = 11.84HH47 pKa = 6.23CLGEE51 pKa = 3.89LDD53 pKa = 5.17FYY55 pKa = 11.39LSPTIFTEE63 pKa = 4.02NPQEE67 pKa = 3.98VAEE70 pKa = 4.48VIVYY74 pKa = 10.25NSEE77 pKa = 3.48VRR79 pKa = 11.84SWINRR84 pKa = 11.84LKK86 pKa = 10.9VEE88 pKa = 4.05YY89 pKa = 10.01EE90 pKa = 3.68RR91 pKa = 11.84LTILSRR97 pKa = 11.84AGSRR101 pKa = 11.84KK102 pKa = 9.52KK103 pKa = 10.83EE104 pKa = 3.52FDD106 pKa = 3.41HH107 pKa = 7.56RR108 pKa = 11.84VGEE111 pKa = 4.23CLKK114 pKa = 10.92LLTAEE119 pKa = 4.79DD120 pKa = 3.6SAAKK124 pKa = 10.05LKK126 pKa = 10.66EE127 pKa = 4.13VIIKK131 pKa = 10.38DD132 pKa = 3.5LEE134 pKa = 4.26GSKK137 pKa = 10.74KK138 pKa = 9.38IDD140 pKa = 3.35PYY142 pKa = 10.66YY143 pKa = 9.87HH144 pKa = 6.19YY145 pKa = 10.16KK146 pKa = 10.64YY147 pKa = 9.91STLEE151 pKa = 3.74PLFEE155 pKa = 4.25EE156 pKa = 4.26MSYY159 pKa = 11.57VSVHH163 pKa = 5.2YY164 pKa = 10.87SEE166 pKa = 5.51VLASRR171 pKa = 11.84LRR173 pKa = 11.84QTASSASKK181 pKa = 11.04SNVEE185 pKa = 4.26EE186 pKa = 4.1VAGDD190 pKa = 3.55ADD192 pKa = 3.76GRR194 pKa = 11.84TQGVEE199 pKa = 3.89QRR201 pKa = 11.84CGDD204 pKa = 3.77DD205 pKa = 3.22VAIGVDD211 pKa = 3.75EE212 pKa = 4.26GHH214 pKa = 6.27SLITGVMSDD223 pKa = 4.36SILPVADD230 pKa = 3.62NYY232 pKa = 11.31AAAKK236 pKa = 8.1EE237 pKa = 3.94NPMYY241 pKa = 10.28NTGKK245 pKa = 9.36EE246 pKa = 3.92WKK248 pKa = 9.95

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7MEB7|J7MEB7_9CLOS Leader protease OS=Blueberry virus A OX=1206566 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.2KK3 pKa = 10.15KK4 pKa = 10.36EE5 pKa = 4.06ITEE8 pKa = 3.99DD9 pKa = 3.39TPLEE13 pKa = 3.99IRR15 pKa = 11.84QKK17 pKa = 9.71LLRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84EE23 pKa = 4.21CNRR26 pKa = 11.84LSSQRR31 pKa = 11.84SRR33 pKa = 11.84DD34 pKa = 3.59NIRR37 pKa = 11.84LKK39 pKa = 10.98KK40 pKa = 10.24EE41 pKa = 3.14IALRR45 pKa = 11.84EE46 pKa = 4.03IEE48 pKa = 4.11NLKK51 pKa = 10.28VLNSSLTVEE60 pKa = 4.64LNEE63 pKa = 4.95LMTNFISINEE73 pKa = 4.03EE74 pKa = 3.54NKK76 pKa = 9.25MLLMQVSYY84 pKa = 10.82LRR86 pKa = 11.84SLLLII91 pKa = 4.42

Molecular weight: 10.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

8619

57

3576

861.9

96.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.903 ± 0.495

2.251 ± 0.195

6.033 ± 0.175

4.815 ± 0.494

4.885 ± 0.283

5.79 ± 0.416

2.123 ± 0.222

5.151 ± 0.138

5.441 ± 0.179

8.992 ± 0.667

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.198

4.478 ± 0.297

3.817 ± 0.384

2.123 ± 0.07

6.381 ± 0.342

9.99 ± 0.223

5.267 ± 0.293

8.62 ± 0.338

0.963 ± 0.099

3.69 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski