Ruaniaceae bacterium KH17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Ruaniaceae; unclassified Ruaniaceae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239QVN1|A0A239QVN1_9MICO Integrase core domain-containing protein OS=Ruaniaceae bacterium KH17 OX=1945888 GN=SAMN06298212_14719 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.21FLRR5 pKa = 11.84AATALAAAALVLTACGSSIPDD26 pKa = 3.71KK27 pKa = 10.41EE28 pKa = 4.39WPTRR32 pKa = 11.84PSTDD36 pKa = 3.02GGTSTDD42 pKa = 4.27DD43 pKa = 3.46ATSSDD48 pKa = 3.95DD49 pKa = 4.6GGIFDD54 pKa = 5.4PLAGTDD60 pKa = 3.28SALAEE65 pKa = 4.59FYY67 pKa = 11.02GQDD70 pKa = 4.42VDD72 pKa = 3.86WQSCGSNLEE81 pKa = 4.28CADD84 pKa = 3.48VVVPLDD90 pKa = 3.67YY91 pKa = 11.36ANPDD95 pKa = 3.26DD96 pKa = 4.0GRR98 pKa = 11.84TVRR101 pKa = 11.84LHH103 pKa = 6.04VGVLGTAGSNSPGLFLNPGGPGGSGFAMLDD133 pKa = 3.59WIEE136 pKa = 4.22HH137 pKa = 5.96SISGSVTSTYY147 pKa = 11.18NLVGFDD153 pKa = 3.55PRR155 pKa = 11.84GVNTSDD161 pKa = 4.15AVSCLSDD168 pKa = 3.58QEE170 pKa = 3.9RR171 pKa = 11.84DD172 pKa = 3.03EE173 pKa = 4.43WNEE176 pKa = 3.74KK177 pKa = 10.65DD178 pKa = 3.4WDD180 pKa = 4.09LEE182 pKa = 4.26TATGFDD188 pKa = 5.0EE189 pKa = 4.2MTADD193 pKa = 3.87YY194 pKa = 10.78KK195 pKa = 10.93WFVDD199 pKa = 3.35KK200 pKa = 11.03CVEE203 pKa = 4.08NTPSDD208 pKa = 3.82LLEE211 pKa = 5.45FIDD214 pKa = 3.92TDD216 pKa = 3.77SAARR220 pKa = 11.84DD221 pKa = 3.62LDD223 pKa = 3.32ILRR226 pKa = 11.84AVVGQQDD233 pKa = 3.53TLDD236 pKa = 3.72YY237 pKa = 11.07LGYY240 pKa = 10.56SYY242 pKa = 10.66GTFLGAQYY250 pKa = 11.52AEE252 pKa = 4.58LFPEE256 pKa = 4.05RR257 pKa = 11.84VGAFVLDD264 pKa = 4.13GALDD268 pKa = 3.91PTLSMQDD275 pKa = 3.1MGKK278 pKa = 8.96EE279 pKa = 3.64QTLGFEE285 pKa = 4.33KK286 pKa = 10.57AIRR289 pKa = 11.84AYY291 pKa = 10.25MEE293 pKa = 4.6DD294 pKa = 3.68CLAGVGCPFSGSVEE308 pKa = 4.11NGLDD312 pKa = 3.41QLNTFFDD319 pKa = 4.16MVTATPLPTSDD330 pKa = 3.99PNRR333 pKa = 11.84DD334 pKa = 3.48LGGSGAISAVLIGLYY349 pKa = 10.04EE350 pKa = 4.13SSLWGEE356 pKa = 3.93ISNALDD362 pKa = 3.18VAMNDD367 pKa = 3.53GDD369 pKa = 4.84GSALQYY375 pKa = 9.38WADD378 pKa = 3.55VSVSRR383 pKa = 11.84LDD385 pKa = 3.89DD386 pKa = 3.37GTYY389 pKa = 10.74EE390 pKa = 4.75DD391 pKa = 4.33NSADD395 pKa = 3.33AFLAITCLDD404 pKa = 3.75YY405 pKa = 11.15PVVGNAADD413 pKa = 3.29WKK415 pKa = 10.7AAAEE419 pKa = 4.1EE420 pKa = 4.24MKK422 pKa = 10.47EE423 pKa = 4.45IAPFWQDD430 pKa = 2.47SFSYY434 pKa = 10.67TEE436 pKa = 4.64VICSQWPHH444 pKa = 4.94EE445 pKa = 4.48AEE447 pKa = 4.0RR448 pKa = 11.84TPAPVTAAGSAPILVIGTTGDD469 pKa = 3.36PATPYY474 pKa = 9.1EE475 pKa = 4.0WSEE478 pKa = 3.91ALVSQLEE485 pKa = 4.2NGHH488 pKa = 6.81LLTFEE493 pKa = 4.89GNGHH497 pKa = 4.95TAYY500 pKa = 10.26GRR502 pKa = 11.84SNSCIQNAVDD512 pKa = 5.35DD513 pKa = 4.31FLLNGTIPAEE523 pKa = 4.23GTTCC527 pKa = 4.29

Molecular weight: 56.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239QTP6|A0A239QTP6_9MICO Cytochrome c-type biogenesis protein OS=Ruaniaceae bacterium KH17 OX=1945888 GN=SAMN06298212_12137 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 9.34RR8 pKa = 11.84RR9 pKa = 11.84ARR11 pKa = 11.84MAKK14 pKa = 8.94KK15 pKa = 9.75KK16 pKa = 9.66HH17 pKa = 5.58RR18 pKa = 11.84KK19 pKa = 8.32RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 7.82TRR25 pKa = 11.84HH26 pKa = 3.36QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2522

0

2522

819933

24

2028

325.1

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.443 ± 0.06

0.611 ± 0.015

5.915 ± 0.045

6.434 ± 0.05

3.17 ± 0.033

8.634 ± 0.04

2.009 ± 0.02

4.995 ± 0.036

2.352 ± 0.037

9.988 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.021

2.397 ± 0.025

5.249 ± 0.035

2.9 ± 0.026

6.684 ± 0.049

5.778 ± 0.032

6.079 ± 0.042

8.544 ± 0.045

1.515 ± 0.022

2.191 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski