Methylobacterium gnaphalii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4699 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512JIV9|A0A512JIV9_9RHIZ Uncharacterized protein OS=Methylobacterium gnaphalii OX=1010610 GN=MGN01_17340 PE=4 SV=1
MM1 pKa = 7.76CCPCGFLCCGSDD13 pKa = 3.18EE14 pKa = 5.23FGGCGCDD21 pKa = 3.4DD22 pKa = 4.57CPDD25 pKa = 3.9PRR27 pKa = 11.84CQEE30 pKa = 4.08QAGEE34 pKa = 4.05AAPVGLPVNDD44 pKa = 4.79DD45 pKa = 4.53DD46 pKa = 5.71EE47 pKa = 5.7LADD50 pKa = 4.92DD51 pKa = 4.71MPLAALPRR59 pKa = 11.84AIRR62 pKa = 11.84RR63 pKa = 11.84FRR65 pKa = 11.84CEE67 pKa = 3.38EE68 pKa = 3.61VRR70 pKa = 11.84PP71 pKa = 3.89

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512JQF4|A0A512JQF4_9RHIZ ModE family transcriptional regulator OS=Methylobacterium gnaphalii OX=1010610 GN=MGN01_40400 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.15VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.62GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.66LSAA44 pKa = 3.99

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4699

0

4699

1334609

40

3311

284.0

30.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.377 ± 0.059

0.838 ± 0.011

5.589 ± 0.028

5.722 ± 0.032

3.428 ± 0.026

8.895 ± 0.037

1.961 ± 0.016

4.772 ± 0.025

3.005 ± 0.035

10.073 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.017

2.293 ± 0.027

5.574 ± 0.033

2.937 ± 0.022

7.773 ± 0.039

5.464 ± 0.024

5.39 ± 0.021

7.448 ± 0.031

1.229 ± 0.016

2.061 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski