Halobonum sp. Gai3-2

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halobaculum

Average proteome isoelectric point is 4.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5GKF7|A0A7D5GKF7_9EURY Uncharacterized protein OS=Halobonum sp. Gai3-2 OX=2743090 GN=HUG10_19290 PE=4 SV=1
MM1 pKa = 7.66TDD3 pKa = 2.9IEE5 pKa = 4.65TTTVCEE11 pKa = 3.83EE12 pKa = 5.05GYY14 pKa = 10.85VCDD17 pKa = 4.3SQMGDD22 pKa = 3.36FNITVDD28 pKa = 3.39ATGDD32 pKa = 3.48EE33 pKa = 4.85GPTANQVLVADD44 pKa = 4.28YY45 pKa = 10.71ASCYY49 pKa = 10.42LPAFRR54 pKa = 11.84AGGQKK59 pKa = 10.21AGHH62 pKa = 7.29DD63 pKa = 4.18DD64 pKa = 3.69LGKK67 pKa = 10.17VQIDD71 pKa = 3.73AEE73 pKa = 4.54ADD75 pKa = 3.5LDD77 pKa = 5.16DD78 pKa = 6.77DD79 pKa = 6.12DD80 pKa = 6.07DD81 pKa = 3.89LTRR84 pKa = 11.84ISFDD88 pKa = 3.75LYY90 pKa = 11.48VEE92 pKa = 4.97ADD94 pKa = 4.09LDD96 pKa = 5.37DD97 pKa = 4.58DD98 pKa = 4.2TLDD101 pKa = 4.02DD102 pKa = 3.99LVARR106 pKa = 11.84GEE108 pKa = 4.67DD109 pKa = 3.08ICHH112 pKa = 4.79VHH114 pKa = 5.58SALRR118 pKa = 11.84EE119 pKa = 4.05GLHH122 pKa = 7.15ADD124 pKa = 3.24ITAHH128 pKa = 7.2ADD130 pKa = 3.13AFF132 pKa = 4.09

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5KM70|A0A7D5KM70_9EURY Uncharacterized protein OS=Halobonum sp. Gai3-2 OX=2743090 GN=HUG10_07815 PE=4 SV=1
MM1 pKa = 7.04NRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84VGTSVYY11 pKa = 10.86AGFLFLVLGLIAWVSGQPFIFPSLGPSAFILAFEE45 pKa = 4.35RR46 pKa = 11.84RR47 pKa = 11.84GEE49 pKa = 3.93RR50 pKa = 11.84TRR52 pKa = 11.84VYY54 pKa = 10.06RR55 pKa = 11.84IVGSHH60 pKa = 6.35VIGGIAGLLAYY71 pKa = 9.69YY72 pKa = 10.05LIASGVSLTATPAGFSPGGLRR93 pKa = 11.84LAASGFISIVLTSWGMIATDD113 pKa = 3.82TNHH116 pKa = 6.96APACATTLIVSLGLLSTPRR135 pKa = 11.84QVAIIVVSVVILAEE149 pKa = 4.04VHH151 pKa = 5.26WAVLASFKK159 pKa = 10.66KK160 pKa = 10.55AVGGTHH166 pKa = 6.58PRR168 pKa = 11.84IGDD171 pKa = 3.6DD172 pKa = 3.14

Molecular weight: 18.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4176

0

4176

1181044

44

2450

282.8

30.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.005 ± 0.056

0.7 ± 0.012

8.349 ± 0.05

8.502 ± 0.058

3.396 ± 0.026

9.108 ± 0.042

2.032 ± 0.021

3.539 ± 0.027

1.679 ± 0.022

8.98 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.778 ± 0.019

2.315 ± 0.024

4.801 ± 0.026

2.185 ± 0.023

6.786 ± 0.04

5.355 ± 0.034

6.247 ± 0.037

9.406 ± 0.044

1.169 ± 0.014

2.669 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski