Campylobacter phage CJIE4-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KXC9|X2KXC9_9CAUD Uncharacterized protein OS=Campylobacter phage CJIE4-3 OX=1470460 GN=00-2425_00021 PE=4 SV=1
MM1 pKa = 7.63PSDD4 pKa = 3.25RR5 pKa = 11.84VEE7 pKa = 4.26IEE9 pKa = 4.47LFTGFYY15 pKa = 10.35DD16 pKa = 3.65KK17 pKa = 10.88KK18 pKa = 10.83GNKK21 pKa = 9.04IYY23 pKa = 10.72EE24 pKa = 4.02GDD26 pKa = 3.19ILYY29 pKa = 10.69SFEE32 pKa = 4.18GCSEE36 pKa = 4.13DD37 pKa = 3.9EE38 pKa = 3.94AFKK41 pKa = 11.17YY42 pKa = 10.35KK43 pKa = 10.76VVFKK47 pKa = 10.74EE48 pKa = 4.07GAFYY52 pKa = 10.71LVEE55 pKa = 5.49CGDD58 pKa = 5.39DD59 pKa = 3.83GEE61 pKa = 4.8EE62 pKa = 3.96WDD64 pKa = 4.64EE65 pKa = 4.27DD66 pKa = 4.12LLSEE70 pKa = 4.6FCLEE74 pKa = 3.87EE75 pKa = 4.35LEE77 pKa = 4.43IVGNIHH83 pKa = 7.0EE84 pKa = 4.43NAEE87 pKa = 4.16LLNEE91 pKa = 4.35NKK93 pKa = 10.19PSS95 pKa = 3.37

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2KRC2|X2KRC2_9CAUD Uncharacterized protein OS=Campylobacter phage CJIE4-3 OX=1470460 GN=00-2425_00013 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84YY3 pKa = 9.98INIKK7 pKa = 8.32PTNKK11 pKa = 9.91SLSNTIIILDD21 pKa = 3.51NSKK24 pKa = 11.13ACFEE28 pKa = 4.28LSKK31 pKa = 10.87KK32 pKa = 9.48NKK34 pKa = 9.25KK35 pKa = 8.39AQNYY39 pKa = 8.35YY40 pKa = 10.23IEE42 pKa = 4.24VQFNGLYY49 pKa = 10.24QPTKK53 pKa = 10.03QIEE56 pKa = 4.19AEE58 pKa = 4.08VRR60 pKa = 11.84KK61 pKa = 9.86ILSKK65 pKa = 9.89MIKK68 pKa = 9.87RR69 pKa = 11.84FKK71 pKa = 10.67AYY73 pKa = 10.3SADD76 pKa = 3.55IACDD80 pKa = 3.54FDD82 pKa = 7.16DD83 pKa = 5.53DD84 pKa = 4.8LAVSKK89 pKa = 10.46PRR91 pKa = 11.84EE92 pKa = 4.29FKK94 pKa = 9.75HH95 pKa = 6.39HH96 pKa = 5.9EE97 pKa = 4.06RR98 pKa = 11.84FTKK101 pKa = 10.67LKK103 pKa = 10.41IFGDD107 pKa = 3.36FHH109 pKa = 6.32TYY111 pKa = 7.34KK112 pKa = 10.5TSMYY116 pKa = 10.23INNPQSKK123 pKa = 8.68YY124 pKa = 10.3YY125 pKa = 9.63KK126 pKa = 10.27LEE128 pKa = 4.99RR129 pKa = 11.84ILLYY133 pKa = 10.59DD134 pKa = 3.35KK135 pKa = 11.34YY136 pKa = 10.55EE137 pKa = 4.17KK138 pKa = 9.94QKK140 pKa = 10.69HH141 pKa = 3.98YY142 pKa = 10.68HH143 pKa = 5.92KK144 pKa = 10.8EE145 pKa = 3.73NIKK148 pKa = 10.62RR149 pKa = 11.84EE150 pKa = 3.97FVRR153 pKa = 11.84WKK155 pKa = 10.49RR156 pKa = 11.84LEE158 pKa = 3.95LTLKK162 pKa = 10.55IKK164 pKa = 10.78DD165 pKa = 3.52KK166 pKa = 10.84FLDD169 pKa = 4.53RR170 pKa = 11.84IEE172 pKa = 4.94NDD174 pKa = 2.98INDD177 pKa = 4.93ALDD180 pKa = 3.84LMQDD184 pKa = 3.84YY185 pKa = 11.15LRR187 pKa = 11.84MIGIWHH193 pKa = 6.55FNMRR197 pKa = 11.84VILEE201 pKa = 3.9QTKK204 pKa = 10.47YY205 pKa = 11.04LNNPRR210 pKa = 11.84WAKK213 pKa = 8.44TFKK216 pKa = 10.38PYY218 pKa = 11.27ALASS222 pKa = 3.67

Molecular weight: 26.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11232

34

1899

193.7

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.903 ± 0.445

1.077 ± 0.23

5.048 ± 0.232

8.77 ± 0.409

5.404 ± 0.323

4.443 ± 0.354

1.051 ± 0.137

8.137 ± 0.308

11.761 ± 0.4

10.158 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.144

7.844 ± 0.371

2.039 ± 0.242

3.784 ± 0.307

2.733 ± 0.357

6.749 ± 0.467

4.594 ± 0.263

3.971 ± 0.309

0.819 ± 0.102

3.517 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski