Rhodococcus phage ChewyVIII

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9EIA3|A0A1C9EIA3_9CAUD Uncharacterized protein OS=Rhodococcus phage ChewyVIII OX=1887657 GN=86 PE=4 SV=1
MM1 pKa = 7.53TLTTNEE7 pKa = 4.24RR8 pKa = 11.84YY9 pKa = 9.76DD10 pKa = 3.17AARR13 pKa = 11.84RR14 pKa = 11.84LISAALGTPRR24 pKa = 11.84DD25 pKa = 3.66DD26 pKa = 3.88ALDD29 pKa = 3.79YY30 pKa = 11.48EE31 pKa = 4.68RR32 pKa = 11.84VTDD35 pKa = 3.55IGVGYY40 pKa = 10.75ASRR43 pKa = 11.84SYY45 pKa = 9.32GTPEE49 pKa = 4.13TVWVLGNWNDD59 pKa = 2.94KK60 pKa = 10.35HH61 pKa = 6.99RR62 pKa = 11.84YY63 pKa = 7.88LDD65 pKa = 3.63EE66 pKa = 6.29GEE68 pKa = 4.36THH70 pKa = 7.41PDD72 pKa = 2.9ADD74 pKa = 3.27QFGRR78 pKa = 11.84VLVDD82 pKa = 3.01GTMSRR87 pKa = 11.84LAEE90 pKa = 4.07ALEE93 pKa = 4.67RR94 pKa = 11.84IDD96 pKa = 6.0VEE98 pKa = 5.32RR99 pKa = 11.84EE100 pKa = 3.72WLDD103 pKa = 3.29EE104 pKa = 4.06WSTCCNCNKK113 pKa = 10.38LIRR116 pKa = 11.84TSADD120 pKa = 3.22SYY122 pKa = 11.57SFTLDD127 pKa = 3.47AVWTIDD133 pKa = 3.95GYY135 pKa = 11.01VCSDD139 pKa = 3.47CAMEE143 pKa = 5.7DD144 pKa = 3.52IEE146 pKa = 5.58NSLIDD151 pKa = 4.48GDD153 pKa = 4.67FVWSVTDD160 pKa = 3.69GAVFEE165 pKa = 4.97PEE167 pKa = 3.78LSRR170 pKa = 11.84VVSFCSTSDD179 pKa = 3.36LEE181 pKa = 5.15AIGFKK186 pKa = 10.38LWTGNPEE193 pKa = 3.67GSTDD197 pKa = 3.29GRR199 pKa = 11.84HH200 pKa = 5.13EE201 pKa = 4.74SGWHH205 pKa = 6.32PGQDD209 pKa = 3.53STPTAIAEE217 pKa = 4.49EE218 pKa = 4.06IAEE221 pKa = 4.59ALDD224 pKa = 3.82CDD226 pKa = 4.08AEE228 pKa = 4.4IVFYY232 pKa = 10.94LDD234 pKa = 3.66EE235 pKa = 4.43NSQFYY240 pKa = 11.04VGFSAWVRR248 pKa = 11.84RR249 pKa = 11.84FDD251 pKa = 3.59WNHH254 pKa = 6.44DD255 pKa = 3.74MTCGVCGRR263 pKa = 11.84TWNDD267 pKa = 2.92EE268 pKa = 4.09NEE270 pKa = 4.25PTPSARR276 pKa = 11.84CPYY279 pKa = 10.4EE280 pKa = 3.73YY281 pKa = 11.01DD282 pKa = 3.22HH283 pKa = 7.85DD284 pKa = 4.5EE285 pKa = 4.62NEE287 pKa = 4.26GDD289 pKa = 3.51NN290 pKa = 3.82

Molecular weight: 32.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9EID6|A0A1C9EID6_9CAUD Uncharacterized protein OS=Rhodococcus phage ChewyVIII OX=1887657 GN=95 PE=4 SV=1
MM1 pKa = 6.3TTAIAPKK8 pKa = 9.66VDD10 pKa = 3.41MVKK13 pKa = 10.42LVSLVKK19 pKa = 10.29RR20 pKa = 11.84NRR22 pKa = 11.84APRR25 pKa = 11.84VGSLVSRR32 pKa = 11.84RR33 pKa = 11.84GAGGPMLYY41 pKa = 9.59TLEE44 pKa = 4.62RR45 pKa = 11.84LDD47 pKa = 4.39EE48 pKa = 4.32LAQGVTVAEE57 pKa = 4.13LRR59 pKa = 11.84MAEE62 pKa = 4.07ADD64 pKa = 3.51SKK66 pKa = 11.23GRR68 pKa = 11.84NRR70 pKa = 11.84LVGWTNTDD78 pKa = 2.69NLTLVRR84 pKa = 11.84FF85 pKa = 4.19

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

21643

40

1614

227.8

25.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.144 ± 0.26

0.781 ± 0.097

7.042 ± 0.189

7.416 ± 0.304

3.396 ± 0.161

8.113 ± 0.524

1.973 ± 0.131

5.027 ± 0.21

5.096 ± 0.23

7.582 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.114

3.636 ± 0.212

5.009 ± 0.182

3.83 ± 0.194

6.348 ± 0.242

5.591 ± 0.199

5.914 ± 0.242

6.593 ± 0.208

1.862 ± 0.126

2.966 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski