Allium cepa amalgavirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; Amalgavirus

Average proteome isoelectric point is 7.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H5MGZ5|A0A2H5MGZ5_9VIRU ORF1+2p OS=Allium cepa amalgavirus 2 OX=2058779 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.62ASSSGPDD8 pKa = 3.11PTQYY12 pKa = 11.62LDD14 pKa = 3.09ITLLSDD20 pKa = 3.88PVAEE24 pKa = 4.38SVSLQDD30 pKa = 4.33AVQHH34 pKa = 6.0LASIGVRR41 pKa = 11.84VEE43 pKa = 3.59RR44 pKa = 11.84FTRR47 pKa = 11.84DD48 pKa = 2.87SIHH51 pKa = 6.77AMRR54 pKa = 11.84MSVPAYY60 pKa = 9.43VKK62 pKa = 10.04EE63 pKa = 3.64IRR65 pKa = 11.84ILGNISDD72 pKa = 4.59RR73 pKa = 11.84EE74 pKa = 4.01LLKK77 pKa = 11.05NVMLQGVKK85 pKa = 10.78NNVLSLPEE93 pKa = 4.53DD94 pKa = 3.56VTPQMVLSFARR105 pKa = 11.84WLKK108 pKa = 9.84RR109 pKa = 11.84GAGARR114 pKa = 11.84VLADD118 pKa = 3.35QQHH121 pKa = 6.27LLKK124 pKa = 10.42INKK127 pKa = 9.06KK128 pKa = 10.14AVGDD132 pKa = 3.92STPDD136 pKa = 2.91IVAFAHH142 pKa = 7.2LLDD145 pKa = 4.1QQVQDD150 pKa = 3.73LTNAKK155 pKa = 9.96KK156 pKa = 10.16RR157 pKa = 11.84VQHH160 pKa = 6.03SSQVRR165 pKa = 11.84IDD167 pKa = 3.75EE168 pKa = 4.08LRR170 pKa = 11.84KK171 pKa = 9.43QIAIEE176 pKa = 3.87EE177 pKa = 4.37HH178 pKa = 5.47NLRR181 pKa = 11.84RR182 pKa = 11.84DD183 pKa = 3.36LAATARR189 pKa = 11.84QFTPANDD196 pKa = 3.48YY197 pKa = 11.22APPSKK202 pKa = 10.57AALDD206 pKa = 3.83NEE208 pKa = 4.36CWEE211 pKa = 4.83LYY213 pKa = 9.72CARR216 pKa = 11.84ARR218 pKa = 11.84DD219 pKa = 3.9AGRR222 pKa = 11.84ALPPWNAILQEE233 pKa = 4.09QATTALSNEE242 pKa = 4.7VINKK246 pKa = 8.53HH247 pKa = 5.72RR248 pKa = 11.84QDD250 pKa = 3.17FCKK253 pKa = 10.29IPSNQRR259 pKa = 11.84LLQTWAASKK268 pKa = 10.55IQDD271 pKa = 3.47LRR273 pKa = 11.84TNQEE277 pKa = 3.6FRR279 pKa = 11.84RR280 pKa = 11.84VTEE283 pKa = 4.04FEE285 pKa = 4.17RR286 pKa = 11.84FLALASGDD294 pKa = 3.28TSMASSSAPEE304 pKa = 4.04AEE306 pKa = 4.42MGQHH310 pKa = 6.98DD311 pKa = 4.1SGGEE315 pKa = 3.92GGEE318 pKa = 4.15SEE320 pKa = 4.35EE321 pKa = 4.64EE322 pKa = 4.17EE323 pKa = 4.49RR324 pKa = 11.84EE325 pKa = 4.11HH326 pKa = 6.13TAAEE330 pKa = 4.26HH331 pKa = 6.25ARR333 pKa = 11.84GGPSEE338 pKa = 4.21QPDD341 pKa = 3.57AEE343 pKa = 4.54GDD345 pKa = 3.8QEE347 pKa = 4.02QTPARR352 pKa = 11.84KK353 pKa = 9.3KK354 pKa = 10.23RR355 pKa = 11.84RR356 pKa = 11.84SKK358 pKa = 10.65RR359 pKa = 11.84LRR361 pKa = 11.84NFPSRR366 pKa = 11.84HH367 pKa = 4.39QRR369 pKa = 11.84SHH371 pKa = 6.25SGSKK375 pKa = 9.16KK376 pKa = 9.54HH377 pKa = 6.09RR378 pKa = 11.84KK379 pKa = 8.65KK380 pKa = 10.46PNRR383 pKa = 11.84RR384 pKa = 11.84DD385 pKa = 3.26SDD387 pKa = 3.71GQEE390 pKa = 3.6

Molecular weight:
43.72 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H5MGZ5|A0A2H5MGZ5_9VIRU ORF1+2p OS=Allium cepa amalgavirus 2 OX=2058779 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.62ASSSGPDD8 pKa = 3.11PTQYY12 pKa = 11.62LDD14 pKa = 3.09ITLLSDD20 pKa = 3.88PVAEE24 pKa = 4.38SVSLQDD30 pKa = 4.33AVQHH34 pKa = 6.0LASIGVRR41 pKa = 11.84VEE43 pKa = 3.59RR44 pKa = 11.84FTRR47 pKa = 11.84DD48 pKa = 2.87SIHH51 pKa = 6.77AMRR54 pKa = 11.84MSVPAYY60 pKa = 9.43VKK62 pKa = 10.04EE63 pKa = 3.64IRR65 pKa = 11.84ILGNISDD72 pKa = 4.59RR73 pKa = 11.84EE74 pKa = 4.01LLKK77 pKa = 11.05NVMLQGVKK85 pKa = 10.78NNVLSLPEE93 pKa = 4.53DD94 pKa = 3.56VTPQMVLSFARR105 pKa = 11.84WLKK108 pKa = 9.84RR109 pKa = 11.84GAGARR114 pKa = 11.84VLADD118 pKa = 3.35QQHH121 pKa = 6.27LLKK124 pKa = 10.42INKK127 pKa = 9.06KK128 pKa = 10.14AVGDD132 pKa = 3.92STPDD136 pKa = 2.91IVAFAHH142 pKa = 7.2LLDD145 pKa = 4.1QQVQDD150 pKa = 3.73LTNAKK155 pKa = 9.96KK156 pKa = 10.16RR157 pKa = 11.84VQHH160 pKa = 6.03SSQVRR165 pKa = 11.84IDD167 pKa = 3.75EE168 pKa = 4.08LRR170 pKa = 11.84KK171 pKa = 9.43QIAIEE176 pKa = 3.87EE177 pKa = 4.37HH178 pKa = 5.47NLRR181 pKa = 11.84RR182 pKa = 11.84DD183 pKa = 3.36LAATARR189 pKa = 11.84QFTPANDD196 pKa = 3.48YY197 pKa = 11.22APPSKK202 pKa = 10.57AALDD206 pKa = 3.83NEE208 pKa = 4.36CWEE211 pKa = 4.83LYY213 pKa = 9.72CARR216 pKa = 11.84ARR218 pKa = 11.84DD219 pKa = 3.9AGRR222 pKa = 11.84ALPPWNAILQEE233 pKa = 4.09QATTALSNEE242 pKa = 4.7VINKK246 pKa = 8.53HH247 pKa = 5.72RR248 pKa = 11.84QDD250 pKa = 3.17FCKK253 pKa = 10.29IPSNQRR259 pKa = 11.84LLQTWAASKK268 pKa = 10.55IQDD271 pKa = 3.47LRR273 pKa = 11.84TNQEE277 pKa = 3.95FVASQSSNDD286 pKa = 3.03SWLSQVEE293 pKa = 4.12THH295 pKa = 6.42LWRR298 pKa = 11.84LPLPLRR304 pKa = 11.84LKK306 pKa = 8.91WANTIPVGKK315 pKa = 9.98VVSRR319 pKa = 11.84KK320 pKa = 9.26RR321 pKa = 11.84RR322 pKa = 11.84NGNILLRR329 pKa = 11.84SMLGADD335 pKa = 4.28LLNNPMQRR343 pKa = 11.84GIKK346 pKa = 9.71NKK348 pKa = 9.96PPLARR353 pKa = 11.84NAEE356 pKa = 4.12VSASVTSLLDD366 pKa = 3.04INARR370 pKa = 11.84ILVLRR375 pKa = 11.84SIEE378 pKa = 3.93RR379 pKa = 11.84SPTVGIPTARR389 pKa = 11.84SRR391 pKa = 11.84FEE393 pKa = 3.61GAVRR397 pKa = 11.84KK398 pKa = 10.18VIGGGEE404 pKa = 4.12MIDD407 pKa = 3.02WRR409 pKa = 11.84TVSNQYY415 pKa = 10.46RR416 pKa = 11.84GGGCFSDD423 pKa = 5.76AILLLADD430 pKa = 4.07ARR432 pKa = 11.84TDD434 pKa = 3.49EE435 pKa = 4.78PGKK438 pKa = 10.29FLPDD442 pKa = 3.85YY443 pKa = 7.85FTLWKK448 pKa = 10.7ARR450 pKa = 11.84DD451 pKa = 3.7ILRR454 pKa = 11.84LPSDD458 pKa = 4.01LKK460 pKa = 11.23VPCNRR465 pKa = 11.84QALKK469 pKa = 10.9VSNFNNDD476 pKa = 2.37ATAGPFFRR484 pKa = 11.84AYY486 pKa = 10.22GIKK489 pKa = 9.94SKK491 pKa = 11.34YY492 pKa = 9.28GMRR495 pKa = 11.84GLLEE499 pKa = 4.27DD500 pKa = 4.93FAWEE504 pKa = 4.76CYY506 pKa = 10.41SSFVDD511 pKa = 3.49NGGDD515 pKa = 3.48VSFLPFVASRR525 pKa = 11.84VGFRR529 pKa = 11.84TKK531 pKa = 10.5LVSQEE536 pKa = 3.55EE537 pKa = 4.13AFIRR541 pKa = 11.84FSKK544 pKa = 10.62NKK546 pKa = 10.31AIGRR550 pKa = 11.84CVMMLDD556 pKa = 4.91AIEE559 pKa = 4.28QMFSSPLYY567 pKa = 10.21NVLSKK572 pKa = 10.01LTADD576 pKa = 4.45LRR578 pKa = 11.84FDD580 pKa = 3.75PASGFRR586 pKa = 11.84NTIVRR591 pKa = 11.84ASSDD595 pKa = 3.05WAKK598 pKa = 9.69MWEE601 pKa = 4.3EE602 pKa = 3.87VKK604 pKa = 10.39KK605 pKa = 10.72AQVIVEE611 pKa = 4.28LDD613 pKa = 2.9WKK615 pKa = 10.85KK616 pKa = 10.69FDD618 pKa = 4.23RR619 pKa = 11.84EE620 pKa = 4.13RR621 pKa = 11.84PTEE624 pKa = 4.25DD625 pKa = 3.38LAFMIDD631 pKa = 3.86VIISCFKK638 pKa = 10.43PEE640 pKa = 3.94NDD642 pKa = 3.7RR643 pKa = 11.84EE644 pKa = 4.18RR645 pKa = 11.84LFLLAYY651 pKa = 7.69KK652 pKa = 10.61TMMQRR657 pKa = 11.84CLIEE661 pKa = 4.33RR662 pKa = 11.84FFVTDD667 pKa = 3.34CGGVFKK673 pKa = 11.33VEE675 pKa = 4.04GMVPSGSLWTGWVDD689 pKa = 3.2TALNILYY696 pKa = 10.45LNAALLSLGFNMTEE710 pKa = 4.18VFPKK714 pKa = 10.71CAGDD718 pKa = 4.12DD719 pKa = 3.7NLTLFMRR726 pKa = 11.84DD727 pKa = 3.04VEE729 pKa = 4.26NRR731 pKa = 11.84RR732 pKa = 11.84LLALKK737 pKa = 10.44DD738 pKa = 3.81RR739 pKa = 11.84LNSWFRR745 pKa = 11.84AGIEE749 pKa = 4.07AEE751 pKa = 4.38DD752 pKa = 4.26FLIHH756 pKa = 6.84RR757 pKa = 11.84PPFFVTRR764 pKa = 11.84EE765 pKa = 3.72QAVFPRR771 pKa = 11.84GTDD774 pKa = 3.45LTKK777 pKa = 10.18GTSKK781 pKa = 10.73IIGNAVWVPIEE792 pKa = 4.08GEE794 pKa = 4.08MVIDD798 pKa = 3.64QEE800 pKa = 4.62AGRR803 pKa = 11.84SHH805 pKa = 6.87RR806 pKa = 11.84WQYY809 pKa = 9.78VFRR812 pKa = 11.84GRR814 pKa = 11.84PKK816 pKa = 10.24FLSAYY821 pKa = 7.8WLEE824 pKa = 4.52DD825 pKa = 3.06GRR827 pKa = 11.84PIRR830 pKa = 11.84PTHH833 pKa = 6.13INSEE837 pKa = 4.08KK838 pKa = 10.75LLFPEE843 pKa = 6.17GIHH846 pKa = 7.39DD847 pKa = 5.6SIDD850 pKa = 3.47TYY852 pKa = 11.03EE853 pKa = 4.18AAVLSMVVDD862 pKa = 3.74NPFNHH867 pKa = 6.78HH868 pKa = 6.66NINHH872 pKa = 5.59CMHH875 pKa = 6.94RR876 pKa = 11.84FVICEE881 pKa = 3.66QVKK884 pKa = 9.08RR885 pKa = 11.84QARR888 pKa = 11.84MGLDD892 pKa = 3.97PIDD895 pKa = 4.17ILWLSRR901 pKa = 11.84FRR903 pKa = 11.84SSVGEE908 pKa = 3.91EE909 pKa = 3.93VPYY912 pKa = 10.93PMIASWRR919 pKa = 11.84RR920 pKa = 11.84QDD922 pKa = 2.73TWVDD926 pKa = 3.43MEE928 pKa = 4.13QLPFVRR934 pKa = 11.84DD935 pKa = 3.42YY936 pKa = 11.76VRR938 pKa = 11.84DD939 pKa = 3.58FRR941 pKa = 11.84EE942 pKa = 4.13FVSGVTSLYY951 pKa = 10.63SRR953 pKa = 11.84QSTGGLDD960 pKa = 3.05SWKK963 pKa = 8.51FTSIIRR969 pKa = 11.84GEE971 pKa = 4.35TEE973 pKa = 3.53FGEE976 pKa = 4.67GQFGNDD982 pKa = 2.67IDD984 pKa = 5.63DD985 pKa = 4.76WISWMYY991 pKa = 9.88RR992 pKa = 11.84NPMTKK997 pKa = 8.74YY998 pKa = 9.55LRR1000 pKa = 11.84PIRR1003 pKa = 11.84RR1004 pKa = 11.84FRR1006 pKa = 11.84AVGEE1010 pKa = 3.94EE1011 pKa = 3.86VRR1013 pKa = 11.84PDD1015 pKa = 3.51RR1016 pKa = 11.84EE1017 pKa = 4.17LSGSLQRR1024 pKa = 11.84CIGIYY1029 pKa = 9.41RR1030 pKa = 11.84QIRR1033 pKa = 11.84GTQEE1037 pKa = 3.5NSSSEE1042 pKa = 3.78NFAIFLSNVLRR1053 pKa = 11.84QTRR1056 pKa = 11.84SYY1058 pKa = 11.09RR1059 pKa = 11.84DD1060 pKa = 3.55ANAHH1064 pKa = 5.57PP1065 pKa = 4.36

Molecular weight:
121.52 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1455

390

1065

727.5

82.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.247 ± 1.152

1.168 ± 0.203

6.323 ± 0.044

5.979 ± 0.741

4.261 ± 0.993

5.361 ± 0.249

2.062 ± 0.647

5.223 ± 0.7

4.674 ± 0.362

9.21 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.276

4.605 ± 0.255

4.742 ± 0.066

4.811 ± 1.074

9.003 ± 0.116

7.629 ± 0.423

4.192 ± 0.046

6.392 ± 0.643

1.993 ± 0.492

1.787 ± 0.387

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski