Aquabacterium pictum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Aquabacterium

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A480B4D9|A0A480B4D9_9BURK Flagellin OS=Aquabacterium pictum OX=2315236 GN=fliC_2 PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PQSTRR7 pKa = 11.84TIYY10 pKa = 10.83AGLALAALLGSGLAQAASSPFQLTGSTDD38 pKa = 3.31SGPLAGTVFSGSFAYY53 pKa = 10.13DD54 pKa = 3.71ASPVVGDD61 pKa = 3.85FSGSVALQAFQLQFAGQTYY80 pKa = 11.03DD81 pKa = 4.06LASADD86 pKa = 3.92APATAMFEE94 pKa = 4.02AGQFIGLDD102 pKa = 3.51YY103 pKa = 11.03TDD105 pKa = 5.27ADD107 pKa = 3.86SADD110 pKa = 3.31AGLRR114 pKa = 11.84PWLSLVPSLTGQFADD129 pKa = 5.13AYY131 pKa = 10.29LAYY134 pKa = 10.39QGADD138 pKa = 3.03GLGGFGSYY146 pKa = 10.16SISAVPEE153 pKa = 4.17PGQWALLLGGLALVGVAGRR172 pKa = 11.84RR173 pKa = 11.84SRR175 pKa = 3.69

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A480AXI6|A0A480AXI6_9BURK RNA polymerase sigma factor OS=Aquabacterium pictum OX=2315236 GN=AQPW35_46970 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSQVV45 pKa = 2.54

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5458

0

5458

1884430

40

6104

345.3

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.704 ± 0.052

0.887 ± 0.011

5.46 ± 0.025

4.254 ± 0.031

3.132 ± 0.019

8.822 ± 0.044

2.285 ± 0.02

3.607 ± 0.025

2.485 ± 0.033

11.451 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.019

2.212 ± 0.023

5.824 ± 0.036

4.45 ± 0.024

7.147 ± 0.042

4.808 ± 0.035

5.268 ± 0.044

7.601 ± 0.03

1.577 ± 0.017

1.816 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski