Ancylobacter pratisalsi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Ancylobacter

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1YK62|A0A6P1YK62_9RHIZ FAD-dependent oxidoreductase OS=Ancylobacter pratisalsi OX=1745854 GN=G3A50_07140 PE=4 SV=1
MM1 pKa = 6.79TTTTEE6 pKa = 4.09LKK8 pKa = 9.17ITLTDD13 pKa = 3.68EE14 pKa = 4.99LQDD17 pKa = 3.59TLNAGGAAVYY27 pKa = 10.08AIYY30 pKa = 10.29FNPTSGAPVIQTLFTGATSAGPATAPITVDD60 pKa = 3.22VPLTLDD66 pKa = 3.48VVSGKK71 pKa = 10.7VYY73 pKa = 10.4FLVQSPVDD81 pKa = 3.61GTLADD86 pKa = 3.55PTTFVGTQSDD96 pKa = 4.82LNWQSAEE103 pKa = 4.02THH105 pKa = 5.98NYY107 pKa = 9.98RR108 pKa = 11.84YY109 pKa = 10.56DD110 pKa = 3.64SFEE113 pKa = 3.86LTIEE117 pKa = 4.01NNINDD122 pKa = 4.44AGNLSSVEE130 pKa = 4.24GYY132 pKa = 9.99GLPMSVGVSYY142 pKa = 11.64GDD144 pKa = 3.73GSSASVGYY152 pKa = 9.87NVSGNEE158 pKa = 3.93LFHH161 pKa = 7.01ALSTMGQAQTVFPYY175 pKa = 9.58ATTAGEE181 pKa = 4.15PGLTGDD187 pKa = 4.44RR188 pKa = 11.84AGLSPSQSVGIKK200 pKa = 9.85SAAFTAGDD208 pKa = 3.26WDD210 pKa = 4.29SYY212 pKa = 11.56VDD214 pKa = 3.86YY215 pKa = 10.66LKK217 pKa = 10.96KK218 pKa = 9.91IDD220 pKa = 4.2PQTHH224 pKa = 5.77EE225 pKa = 4.48PNANAIQFAGFFDD238 pKa = 4.25GAKK241 pKa = 9.97DD242 pKa = 3.66ANGVYY247 pKa = 10.55HH248 pKa = 6.82NGGFYY253 pKa = 10.31AYY255 pKa = 10.48VLEE258 pKa = 4.09WDD260 pKa = 3.9EE261 pKa = 4.22NNGIFWLSPTDD272 pKa = 3.68DD273 pKa = 3.84SQVRR277 pKa = 11.84GYY279 pKa = 10.32IAITPEE285 pKa = 3.69QLAGNIYY292 pKa = 8.74ATEE295 pKa = 4.62GYY297 pKa = 10.34VEE299 pKa = 4.25IYY301 pKa = 9.88EE302 pKa = 4.44SKK304 pKa = 10.74SDD306 pKa = 3.88YY307 pKa = 11.37ASGDD311 pKa = 3.19AYY313 pKa = 10.58HH314 pKa = 7.39IYY316 pKa = 10.67LNTYY320 pKa = 8.31TYY322 pKa = 10.23IDD324 pKa = 3.58SSGKK328 pKa = 10.4SVTNDD333 pKa = 3.09DD334 pKa = 4.83FMDD337 pKa = 4.3AAANNQWGDD346 pKa = 3.16ILKK349 pKa = 10.63DD350 pKa = 3.41LFTGFTAGFYY360 pKa = 11.27GMTGVDD366 pKa = 3.4AQGGQVDD373 pKa = 6.05LDD375 pKa = 4.27QNWNWDD381 pKa = 3.36PTYY384 pKa = 11.46SFGANLATGEE394 pKa = 4.1APIYY398 pKa = 8.89YY399 pKa = 9.51DD400 pKa = 3.7PYY402 pKa = 10.24SAYY405 pKa = 10.39FFANSNSYY413 pKa = 10.64GSGYY417 pKa = 10.3SDD419 pKa = 3.21QLMSQYY425 pKa = 11.14SEE427 pKa = 4.18GGPLISLYY435 pKa = 10.76DD436 pKa = 3.77PSLGGSVTSIAITIFDD452 pKa = 4.95DD453 pKa = 5.04DD454 pKa = 4.33EE455 pKa = 4.73TPTGYY460 pKa = 8.79TKK462 pKa = 10.45PVIYY466 pKa = 10.52NYY468 pKa = 9.68IAPGADD474 pKa = 3.31GYY476 pKa = 9.4TPPEE480 pKa = 3.85YY481 pKa = 10.33DD482 pKa = 3.62ANSAANIVLNFANSAMILDD501 pKa = 3.93EE502 pKa = 4.18TVARR506 pKa = 11.84ITFSFQTGDD515 pKa = 3.72ASHH518 pKa = 7.28PWASVTIDD526 pKa = 3.17GSMGSLWQNWDD537 pKa = 2.8IVQEE541 pKa = 4.11KK542 pKa = 10.02DD543 pKa = 3.0GSYY546 pKa = 10.72SAVPQNPAGMQPAGTILIGKK566 pKa = 9.04LPVADD571 pKa = 5.25DD572 pKa = 4.23GVSHH576 pKa = 6.14YY577 pKa = 10.43KK578 pKa = 10.12IEE580 pKa = 4.02VGANDD585 pKa = 3.42GHH587 pKa = 6.83ASKK590 pKa = 9.64TFNLYY595 pKa = 7.14TTTNADD601 pKa = 3.93GLFLDD606 pKa = 4.72PAYY609 pKa = 10.32AGQAGAIAIDD619 pKa = 3.72GLATVSAAPATQYY632 pKa = 11.5VNTFTIDD639 pKa = 3.74FLPSTTTTIDD649 pKa = 3.24PSLLTRR655 pKa = 11.84VQSTLVPSSVVVGQVTGTDD674 pKa = 3.99PSPSPHH680 pKa = 6.37GGGFTALAGQDD691 pKa = 3.45ALNGNVVSSQHH702 pKa = 5.51AQLGFGWTGLNNATAASASWISGYY726 pKa = 7.35TNKK729 pKa = 9.4VDD731 pKa = 3.88GQSVALVTIAGAGLAPVALLADD753 pKa = 4.02IDD755 pKa = 4.35GQWVSQGKK763 pKa = 9.11SEE765 pKa = 4.21IATLGEE771 pKa = 3.87GTYY774 pKa = 9.93TVTMQQYY781 pKa = 9.09AASDD785 pKa = 3.6TAHH788 pKa = 6.98AHH790 pKa = 6.83PLTQVSSKK798 pKa = 7.75MTLTIALNEE807 pKa = 4.06MDD809 pKa = 5.69LVLAPGGAGAQLVDD823 pKa = 4.79DD824 pKa = 4.96GSGTSGNWIRR834 pKa = 11.84LEE836 pKa = 4.0TSGAALEE843 pKa = 4.77AGSSLVAYY851 pKa = 9.44AVNANGEE858 pKa = 4.36MVSRR862 pKa = 11.84DD863 pKa = 3.4GLEE866 pKa = 4.2AGASVTLQDD875 pKa = 3.75ATLGHH880 pKa = 7.05IGAIAGDD887 pKa = 4.34DD888 pKa = 3.96GSSLMFGGQSVHH900 pKa = 6.65LGAGLEE906 pKa = 4.05LRR908 pKa = 11.84FAEE911 pKa = 4.54IDD913 pKa = 3.52ASGHH917 pKa = 6.47ADD919 pKa = 4.42LSPAMNVSATPDD931 pKa = 3.17GGIQFALDD939 pKa = 3.61GFVLQASVEE948 pKa = 4.14NSLDD952 pKa = 3.4AAAMIAGNQRR962 pKa = 11.84ASDD965 pKa = 4.03TPWVYY970 pKa = 10.94LEE972 pKa = 5.06HH973 pKa = 7.29GDD975 pKa = 4.97LIQFEE980 pKa = 4.63IAGSSANTNTLGFVRR995 pKa = 11.84IDD997 pKa = 3.31VDD999 pKa = 3.67PATGDD1004 pKa = 3.13WSVGGVAYY1012 pKa = 10.49GDD1014 pKa = 3.12TDD1016 pKa = 3.66AFHH1019 pKa = 7.26DD1020 pKa = 4.3AVRR1023 pKa = 11.84GALDD1027 pKa = 5.0DD1028 pKa = 4.21GFLYY1032 pKa = 10.14QQGGNFQVTDD1042 pKa = 3.21EE1043 pKa = 4.52WEE1045 pKa = 3.97VAGASGYY1052 pKa = 8.32YY1053 pKa = 10.47APVLLTQDD1061 pKa = 3.32GEE1063 pKa = 4.71TFVIGNANDD1072 pKa = 3.66GGNDD1076 pKa = 3.83YY1077 pKa = 11.22IRR1079 pKa = 11.84MFGEE1083 pKa = 3.85NTFGIEE1089 pKa = 4.83DD1090 pKa = 3.86LTASAGSDD1098 pKa = 3.17FDD1100 pKa = 4.64YY1101 pKa = 11.45NDD1103 pKa = 3.21MVVRR1107 pKa = 11.84LLPSEE1112 pKa = 4.35EE1113 pKa = 4.14LLSS1116 pKa = 3.86

Molecular weight: 116.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1YMP6|A0A6P1YMP6_9RHIZ Uncharacterized protein OS=Ancylobacter pratisalsi OX=1745854 GN=G3A50_09855 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATSNGRR28 pKa = 11.84KK29 pKa = 8.97ILNARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 4.94GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4319

0

4319

1392111

25

2850

322.3

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.166 ± 0.057

0.786 ± 0.011

5.504 ± 0.028

5.632 ± 0.038

3.694 ± 0.026

8.845 ± 0.035

2.022 ± 0.016

5.014 ± 0.027

2.775 ± 0.032

10.412 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.016

2.317 ± 0.018

5.522 ± 0.031

2.795 ± 0.021

7.353 ± 0.041

5.259 ± 0.023

5.268 ± 0.022

7.762 ± 0.027

1.311 ± 0.016

2.137 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski