Clematis chlorotic mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Pelarspovirus

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1MKB5|A0A1Q1MKB5_9TOMB MP-1 OS=Clematis chlorotic mottle virus OX=1950126 GN=p7 PE=4 SV=1
MM1 pKa = 7.64ASFVSSFLNGLSVLVNVEE19 pKa = 3.99CVLAHH24 pKa = 6.2TLYY27 pKa = 10.39EE28 pKa = 4.29LCTEE32 pKa = 5.04VITQQPSDD40 pKa = 4.14TISAICAAAGSIPADD55 pKa = 3.52PVEE58 pKa = 4.11EE59 pKa = 4.23ALLFTATDD67 pKa = 3.38IQTSLVLEE75 pKa = 5.26DD76 pKa = 5.52DD77 pKa = 4.5LDD79 pKa = 3.51HH80 pKa = 7.23HH81 pKa = 6.94IIKK84 pKa = 10.74EE85 pKa = 4.1EE86 pKa = 3.74VDD88 pKa = 3.17EE89 pKa = 4.83GKK91 pKa = 11.01GEE93 pKa = 4.13EE94 pKa = 4.27VKK96 pKa = 10.65KK97 pKa = 10.16KK98 pKa = 9.93HH99 pKa = 5.54VRR101 pKa = 11.84SRR103 pKa = 11.84VNNRR107 pKa = 11.84VPFAVILAQAAKK119 pKa = 9.97AHH121 pKa = 6.36FGGIPTASRR130 pKa = 11.84ANEE133 pKa = 3.98LSVMKK138 pKa = 10.34YY139 pKa = 9.92LASKK143 pKa = 10.85CEE145 pKa = 4.02EE146 pKa = 4.04LKK148 pKa = 9.61VTPTHH153 pKa = 5.69TRR155 pKa = 11.84ACVTRR160 pKa = 11.84AFPLVFTPDD169 pKa = 3.38EE170 pKa = 4.99DD171 pKa = 3.84DD172 pKa = 3.68LRR174 pKa = 11.84VYY176 pKa = 11.22RR177 pKa = 11.84MLNSEE182 pKa = 4.23NARR185 pKa = 11.84SRR187 pKa = 11.84RR188 pKa = 11.84DD189 pKa = 3.17QYY191 pKa = 11.85LEE193 pKa = 3.68ARR195 pKa = 11.84KK196 pKa = 9.7IPSWWMQLLDD206 pKa = 3.88NPLCARR212 pKa = 11.84AWVRR216 pKa = 11.84MYY218 pKa = 10.93RR219 pKa = 11.84RR220 pKa = 11.84FCGMPDD226 pKa = 2.87EE227 pKa = 5.28KK228 pKa = 11.17AFQFVKK234 pKa = 10.85

Molecular weight: 26.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1MKB7|A0A1Q1MKB7_9TOMB Capsid protein OS=Clematis chlorotic mottle virus OX=1950126 GN=p37 PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 5.0FGEE5 pKa = 4.69SNTTEE10 pKa = 3.93LDD12 pKa = 3.26KK13 pKa = 11.33TKK15 pKa = 10.8TNVGVEE21 pKa = 4.51KK22 pKa = 10.41KK23 pKa = 9.98RR24 pKa = 11.84KK25 pKa = 9.51RR26 pKa = 11.84GSAKK30 pKa = 10.23NKK32 pKa = 10.09LDD34 pKa = 3.43VAHH37 pKa = 6.72NSVGKK42 pKa = 9.75SQSHH46 pKa = 6.92DD47 pKa = 3.34LVGANFITIADD58 pKa = 3.79KK59 pKa = 11.53VKK61 pKa = 9.7FTVHH65 pKa = 5.65LHH67 pKa = 5.42FF68 pKa = 5.76

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1480

68

756

296.0

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.027 ± 0.81

2.568 ± 0.413

5.27 ± 0.333

5.473 ± 1.025

4.595 ± 0.217

6.081 ± 0.784

2.905 ± 0.413

4.595 ± 0.543

5.946 ± 0.821

9.122 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.277

3.919 ± 0.47

4.797 ± 0.512

3.446 ± 0.26

6.486 ± 0.76

7.5 ± 1.506

5.946 ± 0.861

7.432 ± 0.588

1.284 ± 0.264

2.77 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski