Sinorhizobium phage HMSP1-Susan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W204|A0A2D2W204_9CAUD Uncharacterized protein OS=Sinorhizobium phage HMSP1-Susan OX=2049306 GN=HMSP1_40 PE=4 SV=1
MM1 pKa = 7.39PANYY5 pKa = 10.4ALQLRR10 pKa = 11.84EE11 pKa = 4.01ALVTHH16 pKa = 6.59WQNDD20 pKa = 3.61PAVTDD25 pKa = 5.69LIPALQIYY33 pKa = 7.66GVRR36 pKa = 11.84PPANPPRR43 pKa = 11.84PFSRR47 pKa = 11.84VQSLEE52 pKa = 4.08TEE54 pKa = 4.37SYY56 pKa = 10.05EE57 pKa = 3.75ATGYY61 pKa = 10.71DD62 pKa = 3.47GSEE65 pKa = 4.1FPLVRR70 pKa = 11.84VHH72 pKa = 6.69VFAEE76 pKa = 5.0GPDD79 pKa = 3.58EE80 pKa = 4.57VNCSTICNAMQSSIAAASVAGDD102 pKa = 3.65LEE104 pKa = 4.9LIRR107 pKa = 11.84NEE109 pKa = 3.78WLRR112 pKa = 11.84TNIIVDD118 pKa = 3.76GEE120 pKa = 4.27EE121 pKa = 3.48ADD123 pKa = 3.56LWHH126 pKa = 7.02GIIDD130 pKa = 3.79FDD132 pKa = 3.8MAVISRR138 pKa = 11.84IQPP141 pKa = 3.25

Molecular weight: 15.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W2I6|A0A2D2W2I6_9CAUD Uncharacterized protein OS=Sinorhizobium phage HMSP1-Susan OX=2049306 GN=HMSP1_38 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84RR3 pKa = 11.84AIKK6 pKa = 10.49DD7 pKa = 3.37EE8 pKa = 4.02SAKK11 pKa = 10.58EE12 pKa = 3.62LFAAGEE18 pKa = 4.26IIRR21 pKa = 11.84ADD23 pKa = 3.29AQASIRR29 pKa = 11.84EE30 pKa = 4.28GAISGPGHH38 pKa = 6.5IPSLPGEE45 pKa = 4.48PPNADD50 pKa = 3.13THH52 pKa = 6.53NLDD55 pKa = 3.26LSIDD59 pKa = 3.52VRR61 pKa = 11.84LNPSRR66 pKa = 11.84KK67 pKa = 9.04SVLVFARR74 pKa = 11.84AIYY77 pKa = 9.94AAALEE82 pKa = 4.64FGTSRR87 pKa = 11.84IEE89 pKa = 3.59PRR91 pKa = 11.84PFLRR95 pKa = 11.84PALQRR100 pKa = 11.84NRR102 pKa = 11.84NRR104 pKa = 11.84VVYY107 pKa = 9.7GQVQAVRR114 pKa = 11.84NTVRR118 pKa = 11.84VFKK121 pKa = 10.74SDD123 pKa = 3.3SAFTASRR130 pKa = 11.84QRR132 pKa = 11.84YY133 pKa = 7.46EE134 pKa = 4.3DD135 pKa = 3.29SGEE138 pKa = 3.81

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16636

38

930

173.3

19.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.972 ± 0.438

1.034 ± 0.119

5.897 ± 0.241

6.053 ± 0.209

3.847 ± 0.176

7.321 ± 0.223

2.17 ± 0.175

6.041 ± 0.188

4.899 ± 0.221

7.015 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.036 ± 0.168

3.961 ± 0.201

5.164 ± 0.21

3.522 ± 0.191

6.624 ± 0.247

5.007 ± 0.243

6.618 ± 0.313

6.432 ± 0.176

1.731 ± 0.115

3.655 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski