Brevibacterium sp. CS2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium; unclassified Brevibacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8H870|A0A4P8H870_9MICO Uncharacterized protein OS=Brevibacterium sp. CS2 OX=2575923 GN=FDF13_02840 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.1LTTMSKK8 pKa = 10.24VGGVLAASVLALTACGVSNEE28 pKa = 4.46GGNSGGGGDD37 pKa = 3.55EE38 pKa = 4.11TAAGGGSEE46 pKa = 4.77VDD48 pKa = 3.77WSDD51 pKa = 3.43CTPGEE56 pKa = 4.06EE57 pKa = 4.63SADD60 pKa = 3.91TSSMEE65 pKa = 4.46ADD67 pKa = 3.41SDD69 pKa = 3.84TDD71 pKa = 3.35ITIGAFDD78 pKa = 3.56GWDD81 pKa = 3.32EE82 pKa = 4.36SFAAAHH88 pKa = 6.68LYY90 pKa = 10.26KK91 pKa = 10.79YY92 pKa = 10.75LLEE95 pKa = 4.24QDD97 pKa = 4.74GYY99 pKa = 8.53TADD102 pKa = 3.61VQSFQAGPGYY112 pKa = 9.5TGLAEE117 pKa = 4.24GDD119 pKa = 3.05IDD121 pKa = 5.04VILDD125 pKa = 3.4GWLPLTHH132 pKa = 7.31ADD134 pKa = 3.49YY135 pKa = 11.29LEE137 pKa = 4.28QYY139 pKa = 10.86GEE141 pKa = 4.7DD142 pKa = 4.14IEE144 pKa = 6.04AHH146 pKa = 5.65GCWYY150 pKa = 10.47DD151 pKa = 3.62NAKK154 pKa = 8.84LTLAVNEE161 pKa = 4.71DD162 pKa = 3.79SPAQSIADD170 pKa = 3.95LAEE173 pKa = 4.21MGDD176 pKa = 3.73EE177 pKa = 4.05YY178 pKa = 11.57GNRR181 pKa = 11.84IVGIEE186 pKa = 3.93AGAGLTKK193 pKa = 8.78TTEE196 pKa = 3.73EE197 pKa = 4.41SAIPTYY203 pKa = 10.83GLEE206 pKa = 4.24DD207 pKa = 3.56YY208 pKa = 10.76EE209 pKa = 5.44FIVSSTPAMLSEE221 pKa = 4.58LKK223 pKa = 10.53KK224 pKa = 10.7ATDD227 pKa = 3.27SGDD230 pKa = 3.85NILVTLWRR238 pKa = 11.84PHH240 pKa = 4.95WAYY243 pKa = 10.89DD244 pKa = 3.49AFPVRR249 pKa = 11.84DD250 pKa = 3.69LEE252 pKa = 4.43DD253 pKa = 3.97PEE255 pKa = 4.82GAMGEE260 pKa = 4.3AEE262 pKa = 4.81LIYY265 pKa = 10.83NFTRR269 pKa = 11.84SGFSDD274 pKa = 3.66DD275 pKa = 4.15HH276 pKa = 6.55PKK278 pKa = 10.72AGQLLKK284 pKa = 11.11NLVIDD289 pKa = 5.06DD290 pKa = 4.32EE291 pKa = 4.75ALSSLEE297 pKa = 3.39NVMFSEE303 pKa = 4.33EE304 pKa = 4.19NYY306 pKa = 10.7NGEE309 pKa = 4.32DD310 pKa = 3.27LDD312 pKa = 4.26GAVEE316 pKa = 4.34EE317 pKa = 4.53WAGDD321 pKa = 3.38NAEE324 pKa = 5.17FFEE327 pKa = 4.27NWKK330 pKa = 9.48SGSMAGG336 pKa = 3.84

Molecular weight: 35.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8H6Y9|A0A4P8H6Y9_9MICO D-alanyl-D-alanine carboxypeptidase OS=Brevibacterium sp. CS2 OX=2575923 GN=FDF13_00195 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.36GRR40 pKa = 11.84NALSAA45 pKa = 3.9

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2708

0

2708

913174

21

1651

337.2

36.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.382 ± 0.071

0.659 ± 0.012

6.202 ± 0.05

6.279 ± 0.049

3.007 ± 0.028

9.22 ± 0.044

2.233 ± 0.021

4.424 ± 0.029

1.956 ± 0.033

9.756 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.02

1.81 ± 0.022

5.741 ± 0.04

2.718 ± 0.028

7.389 ± 0.058

5.568 ± 0.031

6.197 ± 0.035

8.383 ± 0.047

1.31 ± 0.017

1.863 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski