Pseudomonas phage phi3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Phitrevirus; Pseudomonas virus phi3

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4JP67|A0A0U4JP67_9CAUD Lysozyme OS=Pseudomonas phage phi3 OX=1754217 PE=3 SV=1
MM1 pKa = 7.4NEE3 pKa = 4.24SISAAMAKK11 pKa = 9.79IYY13 pKa = 10.29AYY15 pKa = 10.26DD16 pKa = 3.44QEE18 pKa = 4.66RR19 pKa = 11.84PALIAAGNQALVRR32 pKa = 11.84LAPIALQPTDD42 pKa = 3.26QARR45 pKa = 11.84TIGRR49 pKa = 11.84FLLGLYY55 pKa = 9.97NGEE58 pKa = 4.36EE59 pKa = 4.37FPFDD63 pKa = 3.55LTDD66 pKa = 4.97LRR68 pKa = 11.84GLDD71 pKa = 3.58LDD73 pKa = 5.38LFQDD77 pKa = 4.25CLAVLVMDD85 pKa = 4.55YY86 pKa = 10.99SPEE89 pKa = 4.24LEE91 pKa = 4.09VHH93 pKa = 5.94EE94 pKa = 4.79RR95 pKa = 11.84VPDD98 pKa = 4.05GDD100 pKa = 5.59AIWQRR105 pKa = 11.84LIEE108 pKa = 4.29LWAPEE113 pKa = 3.86AAA115 pKa = 4.45

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4J933|A0A0U4J933_9CAUD Putative head completion/stabilization protein OS=Pseudomonas phage phi3 OX=1754217 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.91EE3 pKa = 4.17LSPSAGSALSGAPQQWHH20 pKa = 7.39DD21 pKa = 3.73IDD23 pKa = 3.24WCRR26 pKa = 11.84VQRR29 pKa = 11.84NVRR32 pKa = 11.84GMQVRR37 pKa = 11.84IAKK40 pKa = 9.92ACRR43 pKa = 11.84EE44 pKa = 4.33GKK46 pKa = 8.2WRR48 pKa = 11.84RR49 pKa = 11.84VKK51 pKa = 10.59ALQRR55 pKa = 11.84MLTRR59 pKa = 11.84STSARR64 pKa = 11.84YY65 pKa = 8.6LAVRR69 pKa = 11.84RR70 pKa = 11.84VTEE73 pKa = 4.01NQGKK77 pKa = 7.83RR78 pKa = 11.84TAGVDD83 pKa = 3.05RR84 pKa = 11.84VLWDD88 pKa = 3.67TPDD91 pKa = 3.98TKK93 pKa = 10.42WKK95 pKa = 10.02AAQGLKK101 pKa = 10.15RR102 pKa = 11.84RR103 pKa = 11.84GYY105 pKa = 8.19QPRR108 pKa = 11.84PLRR111 pKa = 11.84RR112 pKa = 11.84VFIPKK117 pKa = 10.18SNGKK121 pKa = 8.54EE122 pKa = 3.8RR123 pKa = 11.84PLGIPTMTDD132 pKa = 2.45RR133 pKa = 11.84AMQALYY139 pKa = 10.88LLALSPIAEE148 pKa = 4.42TTGDD152 pKa = 3.65PNSYY156 pKa = 9.88GFRR159 pKa = 11.84IEE161 pKa = 5.13RR162 pKa = 11.84STADD166 pKa = 3.07AMSQLFVCLSGKK178 pKa = 10.37ASAQWILEE186 pKa = 3.87ADD188 pKa = 3.15IQGCFDD194 pKa = 5.61HH195 pKa = 7.37INHH198 pKa = 7.1DD199 pKa = 3.34WLLNHH204 pKa = 6.0VPTDD208 pKa = 3.72KK209 pKa = 11.18VILRR213 pKa = 11.84KK214 pKa = 8.71WLKK217 pKa = 10.56AGVIHH222 pKa = 7.52KK223 pKa = 9.9GQLQATDD230 pKa = 3.25AGTPQGGIISPTLANMVLDD249 pKa = 4.09GLEE252 pKa = 4.27SQLKK256 pKa = 8.89RR257 pKa = 11.84HH258 pKa = 6.22LGVTRR263 pKa = 11.84AKK265 pKa = 10.24KK266 pKa = 10.41LKK268 pKa = 10.41LNVVRR273 pKa = 11.84YY274 pKa = 10.11ADD276 pKa = 3.94DD277 pKa = 3.83FVITGVSPEE286 pKa = 4.03VLEE289 pKa = 5.14KK290 pKa = 10.49EE291 pKa = 4.23VKK293 pKa = 9.34PWVEE297 pKa = 3.21QFLAVRR303 pKa = 11.84GLQLSLEE310 pKa = 4.44KK311 pKa = 10.36TRR313 pKa = 11.84IAHH316 pKa = 6.61IDD318 pKa = 3.22QGFDD322 pKa = 3.21FLGWNFRR329 pKa = 11.84KK330 pKa = 10.57YY331 pKa = 10.45NGKK334 pKa = 9.27LLIKK338 pKa = 10.13PSQKK342 pKa = 9.7NAKK345 pKa = 9.59AFYY348 pKa = 9.68GKK350 pKa = 9.94VKK352 pKa = 10.36EE353 pKa = 4.31VIEE356 pKa = 4.2TSRR359 pKa = 11.84TARR362 pKa = 11.84QEE364 pKa = 3.84DD365 pKa = 5.0LIRR368 pKa = 11.84TLNPILRR375 pKa = 11.84GWALYY380 pKa = 8.2HH381 pKa = 5.69QPVVAKK387 pKa = 8.36QTFSRR392 pKa = 11.84MDD394 pKa = 2.92NRR396 pKa = 11.84VFLKK400 pKa = 9.89LWRR403 pKa = 11.84WAKK406 pKa = 9.77RR407 pKa = 11.84RR408 pKa = 11.84HH409 pKa = 5.58PNKK412 pKa = 9.91SLDD415 pKa = 3.47WIRR418 pKa = 11.84RR419 pKa = 11.84RR420 pKa = 11.84YY421 pKa = 9.46FRR423 pKa = 11.84IHH425 pKa = 7.19GDD427 pKa = 3.52KK428 pKa = 9.76TWVFATTVLEE438 pKa = 4.41SNGKK442 pKa = 8.57KK443 pKa = 9.91RR444 pKa = 11.84EE445 pKa = 3.82VALYY449 pKa = 10.19SLASTPIEE457 pKa = 3.87RR458 pKa = 11.84HH459 pKa = 5.24RR460 pKa = 11.84KK461 pKa = 9.15VSGEE465 pKa = 3.89YY466 pKa = 10.25NPYY469 pKa = 10.07DD470 pKa = 3.6PAMEE474 pKa = 5.2AIGEE478 pKa = 4.23KK479 pKa = 10.57LRR481 pKa = 11.84TEE483 pKa = 4.11RR484 pKa = 11.84MLKK487 pKa = 8.95KK488 pKa = 10.4LKK490 pKa = 10.51YY491 pKa = 8.46RR492 pKa = 11.84TQIRR496 pKa = 11.84NLYY499 pKa = 9.65LSQKK503 pKa = 9.56GLCSLCNQPITRR515 pKa = 11.84EE516 pKa = 4.32TGWHH520 pKa = 6.25DD521 pKa = 2.94HH522 pKa = 6.7HH523 pKa = 8.2ILYY526 pKa = 10.22RR527 pKa = 11.84SQGGGDD533 pKa = 3.33SLEE536 pKa = 4.07NRR538 pKa = 11.84VLLHH542 pKa = 6.26PACHH546 pKa = 5.42QQLHH550 pKa = 5.89ARR552 pKa = 11.84GLTVSKK558 pKa = 9.94PARR561 pKa = 3.67

Molecular weight: 64.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

8948

46

896

248.6

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.03 ± 0.57

0.793 ± 0.139

5.767 ± 0.257

6.258 ± 0.346

3.375 ± 0.259

7.432 ± 0.346

2.09 ± 0.216

3.911 ± 0.26

4.079 ± 0.303

10.963 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.212

3.431 ± 0.288

4.973 ± 0.243

5.174 ± 0.315

7.566 ± 0.511

4.906 ± 0.233

5.331 ± 0.296

7.097 ± 0.322

1.598 ± 0.143

2.392 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski