Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus alcalophilus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J8T7M4|J8T7M4_ALKAL Iron transporter OS=Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604 OX=1218173 GN=AJ85_03855 PE=3 SV=1
MM1 pKa = 7.22NNKK4 pKa = 8.94LRR6 pKa = 11.84LLLLVLLASFVVFMAACSNDD26 pKa = 3.51GASGDD31 pKa = 3.99PEE33 pKa = 4.42EE34 pKa = 5.06TPSDD38 pKa = 3.7DD39 pKa = 4.23PEE41 pKa = 4.32TEE43 pKa = 4.02EE44 pKa = 4.27TPEE47 pKa = 4.1EE48 pKa = 4.31DD49 pKa = 3.81EE50 pKa = 4.54EE51 pKa = 4.54TDD53 pKa = 3.26GLYY56 pKa = 10.66SIEE59 pKa = 4.91DD60 pKa = 3.5FDD62 pKa = 5.8HH63 pKa = 6.95IKK65 pKa = 11.05DD66 pKa = 4.31DD67 pKa = 3.59GTTIDD72 pKa = 3.49GGEE75 pKa = 4.16LVFGLSSDD83 pKa = 3.93TPFEE87 pKa = 4.31GTLNWNFYY95 pKa = 10.58SGNPDD100 pKa = 3.18AQVLQWFDD108 pKa = 3.83EE109 pKa = 4.52PLLTWDD115 pKa = 3.48EE116 pKa = 4.56NYY118 pKa = 10.6VYY120 pKa = 9.5TQDD123 pKa = 3.41GAATYY128 pKa = 10.16EE129 pKa = 3.97IDD131 pKa = 4.82EE132 pKa = 4.5EE133 pKa = 4.77DD134 pKa = 3.46NRR136 pKa = 11.84IWTLTIRR143 pKa = 11.84DD144 pKa = 3.82NVNWHH149 pKa = 7.33DD150 pKa = 4.04GTPVTAEE157 pKa = 3.7DD158 pKa = 3.32WAFAHH163 pKa = 6.28YY164 pKa = 10.42VIADD168 pKa = 3.62PDD170 pKa = 3.91YY171 pKa = 11.31DD172 pKa = 3.65GVRR175 pKa = 11.84FDD177 pKa = 3.26SSLRR181 pKa = 11.84NIEE184 pKa = 4.67GIVDD188 pKa = 3.67YY189 pKa = 11.29HH190 pKa = 7.95NGEE193 pKa = 4.02ADD195 pKa = 4.02EE196 pKa = 4.48ISGIEE201 pKa = 4.17IIDD204 pKa = 3.84EE205 pKa = 3.9KK206 pKa = 9.65TLRR209 pKa = 11.84ITYY212 pKa = 8.83VQATPSLITGGVWTYY227 pKa = 11.04PLAKK231 pKa = 9.53HH232 pKa = 6.49IYY234 pKa = 8.65GDD236 pKa = 3.63IPVEE240 pKa = 4.15EE241 pKa = 4.56MSSHH245 pKa = 6.61AATRR249 pKa = 11.84QNPIGFGPYY258 pKa = 9.53KK259 pKa = 10.14VDD261 pKa = 3.7SIVPGEE267 pKa = 4.1SVSYY271 pKa = 10.57SKK273 pKa = 11.65YY274 pKa = 7.66EE275 pKa = 4.06DD276 pKa = 3.52YY277 pKa = 10.71WRR279 pKa = 11.84GEE281 pKa = 3.8PALDD285 pKa = 3.33KK286 pKa = 10.56VTLRR290 pKa = 11.84VINPSVVVEE299 pKa = 3.91EE300 pKa = 4.37LRR302 pKa = 11.84NGGVDD307 pKa = 3.39MVSSFPQAQFADD319 pKa = 4.12VVDD322 pKa = 4.17EE323 pKa = 4.59LSNITWLGNIDD334 pKa = 3.59RR335 pKa = 11.84AYY337 pKa = 9.76TYY339 pKa = 10.47IGFKK343 pKa = 10.12LGHH346 pKa = 6.36WDD348 pKa = 3.13EE349 pKa = 4.37EE350 pKa = 4.41NRR352 pKa = 11.84VNVYY356 pKa = 10.56DD357 pKa = 4.15PEE359 pKa = 4.11NAKK362 pKa = 9.68MGNKK366 pKa = 9.27NLRR369 pKa = 11.84QAMWHH374 pKa = 6.19AVDD377 pKa = 4.01NDD379 pKa = 4.18TVGEE383 pKa = 4.07RR384 pKa = 11.84FYY386 pKa = 11.27HH387 pKa = 5.77GLRR390 pKa = 11.84WAGTTLIPPSHH401 pKa = 7.25PEE403 pKa = 3.53FHH405 pKa = 7.6DD406 pKa = 3.32ATNPGRR412 pKa = 11.84AYY414 pKa = 10.58DD415 pKa = 3.76PEE417 pKa = 4.3RR418 pKa = 11.84ANEE421 pKa = 4.09LLDD424 pKa = 3.41EE425 pKa = 4.96AGYY428 pKa = 11.28VDD430 pKa = 3.81VDD432 pKa = 3.24GDD434 pKa = 4.08GFRR437 pKa = 11.84EE438 pKa = 4.26DD439 pKa = 3.74PDD441 pKa = 4.02GNEE444 pKa = 3.83LVINFASMSGDD455 pKa = 3.64EE456 pKa = 4.22IAEE459 pKa = 4.06PLANYY464 pKa = 8.64YY465 pKa = 8.96IQAWANVGLNVQLLDD480 pKa = 3.91GRR482 pKa = 11.84LHH484 pKa = 6.49EE485 pKa = 5.13FNSFYY490 pKa = 11.31DD491 pKa = 3.47RR492 pKa = 11.84VEE494 pKa = 4.91ADD496 pKa = 4.8DD497 pKa = 4.81PDD499 pKa = 4.01IDD501 pKa = 3.81IYY503 pKa = 10.86QGAWVVGIDD512 pKa = 3.62VDD514 pKa = 4.23PSGLYY519 pKa = 10.12GAEE522 pKa = 3.24ASYY525 pKa = 11.38NYY527 pKa = 8.89TRR529 pKa = 11.84YY530 pKa = 10.52VSDD533 pKa = 4.16EE534 pKa = 3.94NNQLMTDD541 pKa = 4.32GLSEE545 pKa = 3.95AAFDD549 pKa = 3.04VDD551 pKa = 3.95YY552 pKa = 11.13RR553 pKa = 11.84VDD555 pKa = 5.23VYY557 pKa = 11.14NQWQEE562 pKa = 3.92YY563 pKa = 7.28MVEE566 pKa = 4.18NVPAFPTLYY575 pKa = 10.03RR576 pKa = 11.84AALVPVNNRR585 pKa = 11.84VTGYY589 pKa = 11.13AVGDD593 pKa = 3.44GTGYY597 pKa = 10.15FRR599 pKa = 11.84YY600 pKa = 9.42QVGVTEE606 pKa = 4.67EE607 pKa = 4.12VAEE610 pKa = 4.23SAEE613 pKa = 4.02

Molecular weight: 68.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094WK42|A0A094WK42_ALKAL Cold-shock protein OS=Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604 OX=1218173 GN=AJ85_03455 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3743

0

3743

1064280

26

1694

284.3

32.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.776 ± 0.045

0.69 ± 0.013

4.979 ± 0.031

8.167 ± 0.05

4.62 ± 0.03

6.656 ± 0.039

2.117 ± 0.019

7.722 ± 0.039

6.681 ± 0.04

10.094 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.019

4.325 ± 0.028

3.474 ± 0.021

4.054 ± 0.029

4.087 ± 0.031

5.922 ± 0.03

5.318 ± 0.024

7.068 ± 0.034

1.049 ± 0.015

3.481 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski