Streptomyces sp. 3213.3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5BRP4|A0A1H5BRP4_9ACTN Uncharacterized protein OS=Streptomyces sp. 3213.3 OX=1855348 GN=SAMN05216489_03811 PE=4 SV=1
MM1 pKa = 5.91VTEE4 pKa = 4.54WPVVTTAAFSGTASASGARR23 pKa = 11.84SGSVRR28 pKa = 11.84RR29 pKa = 11.84VGVGGSLAVDD39 pKa = 4.41DD40 pKa = 4.86PASGSSDD47 pKa = 3.01AGGSGIGDD55 pKa = 4.03LVDD58 pKa = 4.79AMASLTLSGFDD69 pKa = 3.31TYY71 pKa = 11.54EE72 pKa = 4.01SAEE75 pKa = 4.1QAAEE79 pKa = 4.03DD80 pKa = 4.0ALGDD84 pKa = 3.94GSDD87 pKa = 3.96TEE89 pKa = 4.62MPDD92 pKa = 3.19APGGGDD98 pKa = 3.12VGHH101 pKa = 7.68PDD103 pKa = 3.7SPHH106 pKa = 6.98SSGDD110 pKa = 3.62DD111 pKa = 3.3PATAGGEE118 pKa = 4.16CDD120 pKa = 3.87VPDD123 pKa = 4.05PTGHH127 pKa = 6.71TSHH130 pKa = 7.91PDD132 pKa = 3.4PRR134 pKa = 11.84DD135 pKa = 3.01TAANPEE141 pKa = 4.64SNFDD145 pKa = 4.17GIPDD149 pKa = 3.56YY150 pKa = 11.18VQYY153 pKa = 10.81RR154 pKa = 11.84DD155 pKa = 3.8SNEE158 pKa = 3.45PLYY161 pKa = 10.97RR162 pKa = 11.84SDD164 pKa = 3.57TRR166 pKa = 11.84DD167 pKa = 2.94PSIIFNQGFQAKK179 pKa = 9.4NPPNADD185 pKa = 2.59LWNYY189 pKa = 10.24VLTNQPAEE197 pKa = 4.3YY198 pKa = 10.59VSTTRR203 pKa = 11.84SDD205 pKa = 3.5NLNWGPQYY213 pKa = 11.13VYY215 pKa = 10.52DD216 pKa = 3.58IRR218 pKa = 11.84APGGIDD224 pKa = 3.18VNATYY229 pKa = 10.37GSHH232 pKa = 6.35SPYY235 pKa = 10.23PEE237 pKa = 3.72QEE239 pKa = 4.06EE240 pKa = 4.19IAFPGGIKK248 pKa = 9.92PEE250 pKa = 4.28YY251 pKa = 9.89IKK253 pKa = 10.85GVWQMNPDD261 pKa = 3.71GTHH264 pKa = 5.47GQYY267 pKa = 10.1IPNPNYY273 pKa = 9.92NAGAAGSNAAPVDD286 pKa = 3.82PPNWTDD292 pKa = 3.5SDD294 pKa = 3.68VDD296 pKa = 3.94MPDD299 pKa = 3.57ADD301 pKa = 4.24TPLPSSPDD309 pKa = 3.3PMDD312 pKa = 3.91IDD314 pKa = 3.69

Molecular weight: 32.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5FG86|A0A1H5FG86_9ACTN NADP-dependent 3-hydroxy acid dehydrogenase YdfG OS=Streptomyces sp. 3213.3 OX=1855348 GN=SAMN05216489_05247 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9510

0

9510

3163011

26

10035

332.6

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.203 ± 0.034

0.77 ± 0.007

6.0 ± 0.022

5.431 ± 0.029

2.783 ± 0.015

9.337 ± 0.026

2.308 ± 0.013

3.262 ± 0.018

2.264 ± 0.022

10.266 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.711 ± 0.011

1.945 ± 0.016

5.974 ± 0.029

2.852 ± 0.015

7.652 ± 0.031

5.39 ± 0.022

6.59 ± 0.031

8.485 ± 0.026

1.566 ± 0.01

2.211 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski