Staphylococcus phage IME1361_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus IME1361-01

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JPK7|A0A1W6JPK7_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1361_01 OX=1965490 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.66EE41 pKa = 4.34ILEE44 pKa = 4.2GLPNAMQDD52 pKa = 3.36ALKK55 pKa = 10.66EE56 pKa = 4.16DD57 pKa = 3.68IYY59 pKa = 11.25LDD61 pKa = 3.66EE62 pKa = 5.14AVGIMTSQVVYY73 pKa = 10.27KK74 pKa = 10.8YY75 pKa = 10.75EE76 pKa = 3.86EE77 pKa = 4.05EE78 pKa = 4.32QEE80 pKa = 4.17NEE82 pKa = 3.77

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JPM5|A0A1W6JPM5_9CAUD Structural protein OS=Staphylococcus phage IME1361_01 OX=1965490 PE=4 SV=1
MM1 pKa = 7.13VNKK4 pKa = 9.9INKK7 pKa = 8.26FNQYY11 pKa = 6.91VARR14 pKa = 11.84FIPYY18 pKa = 9.11IATKK22 pKa = 10.35IPIKK26 pKa = 10.71KK27 pKa = 10.0NIAPMIKK34 pKa = 9.64QKK36 pKa = 11.1SDD38 pKa = 3.15AEE40 pKa = 4.16LLKK43 pKa = 11.22NEE45 pKa = 4.09VFRR48 pKa = 11.84VRR50 pKa = 11.84KK51 pKa = 9.44

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12711

45

1509

189.7

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.491 ± 0.401

0.535 ± 0.092

6.207 ± 0.264

8.308 ± 0.534

4.327 ± 0.281

5.46 ± 0.602

1.676 ± 0.178

7.985 ± 0.31

9.834 ± 0.357

8.143 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.168

6.742 ± 0.323

2.62 ± 0.161

3.761 ± 0.277

4.248 ± 0.242

5.782 ± 0.257

5.539 ± 0.212

5.546 ± 0.275

1.078 ± 0.162

4.335 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski