Streptomyces yanglinensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5Y137|A0A1H5Y137_9ACTN Protein-tyrosine phosphatase OS=Streptomyces yanglinensis OX=310779 GN=SAMN05216223_103511 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.54FSGRR8 pKa = 11.84RR9 pKa = 11.84AFRR12 pKa = 11.84AASALTLSLGAGLAAPLLAAGSAGAATPSATAGLSDD48 pKa = 3.53NAIVYY53 pKa = 7.66TAAAGQANKK62 pKa = 8.46VTVTSSLTDD71 pKa = 3.32GNTKK75 pKa = 7.78VTYY78 pKa = 10.7VIDD81 pKa = 4.04DD82 pKa = 3.9VVPITAGDD90 pKa = 3.77GCTYY94 pKa = 7.71PTSTDD99 pKa = 3.09HH100 pKa = 6.69TKK102 pKa = 10.53VSCAAPMLDD111 pKa = 4.08TEE113 pKa = 5.03DD114 pKa = 4.2PYY116 pKa = 11.66ATLEE120 pKa = 4.09LALGDD125 pKa = 3.76GDD127 pKa = 5.63DD128 pKa = 3.88AVTYY132 pKa = 11.26ANGTNQAYY140 pKa = 9.89YY141 pKa = 10.28FARR144 pKa = 11.84FDD146 pKa = 4.32LGDD149 pKa = 3.85GKK151 pKa = 9.68DD152 pKa = 3.48TYY154 pKa = 11.22TEE156 pKa = 3.98TGKK159 pKa = 10.08VQGNDD164 pKa = 3.35VLGGAGDD171 pKa = 3.85DD172 pKa = 3.83TLTVSSYY179 pKa = 9.79TVALGGDD186 pKa = 4.06DD187 pKa = 4.64NDD189 pKa = 4.92TIHH192 pKa = 7.15AGADD196 pKa = 3.6TIVQGGNGNDD206 pKa = 3.41TVDD209 pKa = 3.43ASGAGSNVDD218 pKa = 3.27GGAGNDD224 pKa = 3.89VINGGDD230 pKa = 3.5GRR232 pKa = 11.84QDD234 pKa = 3.41LSGGDD239 pKa = 4.14GNDD242 pKa = 3.5KK243 pKa = 10.66IYY245 pKa = 10.91GGKK248 pKa = 10.4GDD250 pKa = 4.09DD251 pKa = 3.73FLYY254 pKa = 10.68GGKK257 pKa = 10.35GDD259 pKa = 5.28DD260 pKa = 3.32ILYY263 pKa = 10.65GNSGNDD269 pKa = 3.44TIYY272 pKa = 11.22GNSGNDD278 pKa = 3.47GVYY281 pKa = 10.48GGPGTDD287 pKa = 3.54TLSGGPGTNIVHH299 pKa = 6.49QDD301 pKa = 2.88

Molecular weight: 29.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6BU77|A0A1H6BU77_9ACTN Uncharacterized protein OS=Streptomyces yanglinensis OX=310779 GN=SAMN05216223_107243 PE=4 SV=1
MM1 pKa = 6.39TTVPVARR8 pKa = 11.84AVMTTAVAPATVGTTAGMTVPATTVLVMTAPATTVPAVPVVASAVMTARR57 pKa = 11.84VMTAPAVRR65 pKa = 11.84VVMTVLPGVAASAVTTVPVAVPVVGSVAMTVPAVPVVASAVMTARR110 pKa = 11.84VMTVGTTARR119 pKa = 11.84AVRR122 pKa = 11.84VVMTVLPGVAASAVTTVPVAVPVVGSVAMTVPVAVPVVGSVVTTVPAVPVVASAVTTGRR181 pKa = 11.84TGRR184 pKa = 11.84ATTVPVTIVPVAAPVSVGTTARR206 pKa = 11.84VMTARR211 pKa = 11.84VTTVRR216 pKa = 11.84AVTTTVVVSAATTAVTTVPVVRR238 pKa = 11.84AAMTTVAVSVAMTGRR253 pKa = 11.84TGRR256 pKa = 11.84ATTVPVTIVPVAAPVSVGTTARR278 pKa = 11.84VMTARR283 pKa = 11.84VTTVRR288 pKa = 11.84AVTTTVVVSAATTAVTTVPVVRR310 pKa = 11.84AAMTTVAVSVAMTGRR325 pKa = 11.84TGRR328 pKa = 11.84ATTVPVTIVPVAAPVSVGTTARR350 pKa = 11.84VMTARR355 pKa = 11.84ATTAGTSVLATTVPAGSVVTTVRR378 pKa = 11.84TGRR381 pKa = 11.84VMTVGTTARR390 pKa = 11.84AVRR393 pKa = 11.84GVMTVPPGVVASAVMTARR411 pKa = 11.84VTTVVTTGGTTVRR424 pKa = 11.84PVRR427 pKa = 11.84AGTVTGSAAAAGARR441 pKa = 11.84WCRR444 pKa = 11.84AAAGSRR450 pKa = 11.84AAAATTTAGTTGNRR464 pKa = 11.84SGGCRR469 pKa = 11.84SRR471 pKa = 11.84TTT473 pKa = 3.13

Molecular weight: 45.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8055

0

8055

2771259

26

5593

344.0

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.194 ± 0.041

0.756 ± 0.008

6.039 ± 0.02

5.06 ± 0.029

2.67 ± 0.016

9.886 ± 0.03

2.323 ± 0.013

2.987 ± 0.018

1.777 ± 0.018

10.076 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.01

1.775 ± 0.015

6.425 ± 0.027

2.821 ± 0.018

7.859 ± 0.035

5.35 ± 0.023

6.333 ± 0.029

8.398 ± 0.023

1.516 ± 0.011

2.098 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski