Pepper golden mosaic virus-[CR]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Pepper golden mosaic virus

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9QJK6|Q9QJK6_9GEMI Transcriptional activator protein OS=Pepper golden mosaic virus-[CR] OX=223302 GN=AC2 PE=3 SV=1
MM1 pKa = 7.93PLPPKK6 pKa = 10.4SFHH9 pKa = 6.14LQCKK13 pKa = 10.13NIFLTYY19 pKa = 8.19PQCDD23 pKa = 3.41IPKK26 pKa = 10.43DD27 pKa = 3.5EE28 pKa = 4.92ALEE31 pKa = 3.94MLQSLKK37 pKa = 10.36WSVVKK42 pKa = 10.05PIYY45 pKa = 10.04IRR47 pKa = 11.84VSRR50 pKa = 11.84EE51 pKa = 3.46EE52 pKa = 4.04HH53 pKa = 6.5SDD55 pKa = 3.62GFPHH59 pKa = 6.6LHH61 pKa = 6.91CLIQLTGKK69 pKa = 10.57CNIKK73 pKa = 10.14DD74 pKa = 3.38ARR76 pKa = 11.84FFDD79 pKa = 3.48ITHH82 pKa = 7.1PRR84 pKa = 11.84RR85 pKa = 11.84SAQFHH90 pKa = 6.88PNVQAAKK97 pKa = 9.12DD98 pKa = 3.63TNAVKK103 pKa = 10.64NYY105 pKa = 6.5ITKK108 pKa = 10.81DD109 pKa = 2.87GDD111 pKa = 3.67YY112 pKa = 11.07CEE114 pKa = 4.23TGQYY118 pKa = 9.68KK119 pKa = 10.39VSGGSKK125 pKa = 9.95ANKK128 pKa = 9.55DD129 pKa = 3.77DD130 pKa = 4.11VYY132 pKa = 11.43HH133 pKa = 6.34NAVNAASAGEE143 pKa = 3.84ALDD146 pKa = 4.26IIKK149 pKa = 10.81AGDD152 pKa = 3.56PRR154 pKa = 11.84TFIVSYY160 pKa = 11.0HH161 pKa = 4.97NVKK164 pKa = 10.87SNIEE168 pKa = 4.05RR169 pKa = 11.84LFKK172 pKa = 10.52PPPKK176 pKa = 9.79QWTPPYY182 pKa = 9.66PLSSFNNVPEE192 pKa = 4.22DD193 pKa = 3.46MQRR196 pKa = 11.84CVAEE200 pKa = 3.84YY201 pKa = 10.13FGRR204 pKa = 11.84SSAARR209 pKa = 11.84PDD211 pKa = 3.51RR212 pKa = 11.84PISIIIEE219 pKa = 4.12GDD221 pKa = 3.42SRR223 pKa = 11.84SGKK226 pKa = 7.69TMWARR231 pKa = 11.84ALGPHH236 pKa = 6.63NYY238 pKa = 10.14LSGHH242 pKa = 6.97LDD244 pKa = 3.71FNSKK248 pKa = 10.15VYY250 pKa = 11.23SNDD253 pKa = 2.67VEE255 pKa = 4.6YY256 pKa = 11.36NVIDD260 pKa = 5.95DD261 pKa = 4.05ITPHH265 pKa = 5.11YY266 pKa = 10.58LKK268 pKa = 10.62LKK270 pKa = 9.09HH271 pKa = 5.7WKK273 pKa = 9.57EE274 pKa = 4.04LIGAQRR280 pKa = 11.84DD281 pKa = 3.53WQSNCKK287 pKa = 9.41YY288 pKa = 10.21GKK290 pKa = 9.18PVQIKK295 pKa = 10.19GGIPSIVLCNPGEE308 pKa = 4.25GSSYY312 pKa = 10.53IAFLNKK318 pKa = 9.95EE319 pKa = 3.85EE320 pKa = 4.09NAPLRR325 pKa = 11.84AWTQKK330 pKa = 9.91NAQFVILHH338 pKa = 6.13APLYY342 pKa = 10.56KK343 pKa = 9.81STAQDD348 pKa = 3.02SS349 pKa = 3.81

Molecular weight: 39.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QJK8|Q9QJK8_9GEMI Capsid protein OS=Pepper golden mosaic virus-[CR] OX=223302 GN=AV1 PE=3 SV=1
MM1 pKa = 8.25DD2 pKa = 4.26SRR4 pKa = 11.84TGEE7 pKa = 4.39SITVRR12 pKa = 11.84QAEE15 pKa = 3.98NSVFIWKK22 pKa = 9.56VPNPLFFKK30 pKa = 9.82IQRR33 pKa = 11.84VEE35 pKa = 4.01APLDD39 pKa = 3.4TRR41 pKa = 11.84TRR43 pKa = 11.84IYY45 pKa = 10.5YY46 pKa = 9.57IEE48 pKa = 3.83VRR50 pKa = 11.84FNHH53 pKa = 5.71NLRR56 pKa = 11.84RR57 pKa = 11.84ALALHH62 pKa = 6.52KK63 pKa = 10.67SFLHH67 pKa = 4.9FQVWTTSMTASGTTYY82 pKa = 10.97LNRR85 pKa = 11.84FKK87 pKa = 10.85FRR89 pKa = 11.84IMLYY93 pKa = 8.49LHH95 pKa = 7.03RR96 pKa = 11.84LGAIGINNVIRR107 pKa = 11.84AVRR110 pKa = 11.84FATDD114 pKa = 3.02KK115 pKa = 11.32SYY117 pKa = 11.46VNYY120 pKa = 10.16VLEE123 pKa = 4.03HH124 pKa = 6.88HH125 pKa = 6.46EE126 pKa = 4.36IKK128 pKa = 10.88YY129 pKa = 10.66KK130 pKa = 10.9LYY132 pKa = 10.88

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

861

129

349

215.3

24.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.156 ± 0.601

1.974 ± 0.449

5.226 ± 0.736

3.717 ± 0.377

4.878 ± 0.748

5.459 ± 0.557

3.833 ± 0.44

6.039 ± 0.893

6.62 ± 0.687

6.039 ± 0.888

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.836

6.039 ± 0.331

5.807 ± 1.205

3.717 ± 0.56

7.201 ± 1.315

7.666 ± 1.028

4.762 ± 0.795

6.272 ± 1.096

1.626 ± 0.185

4.762 ± 0.901
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski