Campylobacter phage DA10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649WKF0|A0A649WKF0_9CAUD Uncharacterized protein OS=Campylobacter phage DA10 OX=2656519 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.59LKK4 pKa = 10.96DD5 pKa = 3.41FDD7 pKa = 4.01FRR9 pKa = 11.84IWSYY13 pKa = 11.44NIEE16 pKa = 4.51EE17 pKa = 4.13YY18 pKa = 10.64LQPKK22 pKa = 7.77NTYY25 pKa = 9.22LAIVKK30 pKa = 9.74DD31 pKa = 4.08EE32 pKa = 4.75KK33 pKa = 11.48GLDD36 pKa = 3.59SYY38 pKa = 11.9EE39 pKa = 4.22KK40 pKa = 10.77VLDD43 pKa = 3.9VADD46 pKa = 4.45DD47 pKa = 4.19CSGHH51 pKa = 7.48DD52 pKa = 3.85YY53 pKa = 11.04PLYY56 pKa = 10.56QYY58 pKa = 10.63GDD60 pKa = 3.88SVNIVYY66 pKa = 10.58KK67 pKa = 11.14NEE69 pKa = 4.61DD70 pKa = 3.6DD71 pKa = 6.74DD72 pKa = 4.49ILQEE76 pKa = 4.0IQTSGEE82 pKa = 3.9

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649WKE9|A0A649WKE9_9CAUD Uncharacterized protein OS=Campylobacter phage DA10 OX=2656519 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 10.29ILLRR6 pKa = 11.84KK7 pKa = 9.61NKK9 pKa = 9.14IRR11 pKa = 11.84LSYY14 pKa = 10.29IIPNFNDD21 pKa = 3.16RR22 pKa = 11.84SALFNFISIKK32 pKa = 10.04RR33 pKa = 11.84KK34 pKa = 9.55KK35 pKa = 10.58LLDD38 pKa = 3.32SFEE41 pKa = 4.47RR42 pKa = 11.84FNGAIYY48 pKa = 10.58LSANDD53 pKa = 3.15IKK55 pKa = 11.04KK56 pKa = 10.31IIRR59 pKa = 11.84LNDD62 pKa = 3.23KK63 pKa = 10.18QDD65 pKa = 2.96NSKK68 pKa = 9.69ICKK71 pKa = 9.53RR72 pKa = 11.84VNAVNLLKK80 pKa = 10.47EE81 pKa = 4.09YY82 pKa = 10.49LKK84 pKa = 11.02KK85 pKa = 10.89GVIKK89 pKa = 10.76

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11088

31

515

187.9

21.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.186 ± 0.34

1.218 ± 0.118

6.034 ± 0.273

6.99 ± 0.356

5.817 ± 0.231

4.87 ± 0.279

0.64 ± 0.087

8.92 ± 0.346

9.758 ± 0.47

9.416 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.124

9.001 ± 0.337

2.318 ± 0.149

3.427 ± 0.163

2.733 ± 0.169

6.638 ± 0.308

4.987 ± 0.32

4.87 ± 0.259

0.712 ± 0.079

4.374 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski