Flavobacterium akiainvivens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3833 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9VK35|A0A0M9VK35_9FLAO Uncharacterized protein OS=Flavobacterium akiainvivens OX=1202724 GN=AM493_20005 PE=4 SV=1
MM1 pKa = 7.11ITACSDD7 pKa = 3.86DD8 pKa = 5.74DD9 pKa = 4.58NATNNNNVTRR19 pKa = 11.84AQVVEE24 pKa = 4.43NYY26 pKa = 11.13ADD28 pKa = 3.05IVYY31 pKa = 10.62QNYY34 pKa = 9.71QDD36 pKa = 4.57ALDD39 pKa = 4.36DD40 pKa = 4.35AEE42 pKa = 4.34SLEE45 pKa = 4.28VAVNAFTANPTQATFDD61 pKa = 3.59AAKK64 pKa = 10.21AAWKK68 pKa = 8.95TSRR71 pKa = 11.84EE72 pKa = 4.29SYY74 pKa = 9.09GTTEE78 pKa = 4.23AFRR81 pKa = 11.84FANGPIDD88 pKa = 3.8NDD90 pKa = 3.28QDD92 pKa = 3.67FEE94 pKa = 4.55APEE97 pKa = 4.07GLINAWPLDD106 pKa = 3.8EE107 pKa = 6.3AYY109 pKa = 9.64IDD111 pKa = 3.84YY112 pKa = 10.68VQASDD117 pKa = 3.2AQYY120 pKa = 11.42NGGIINNTEE129 pKa = 4.47LYY131 pKa = 8.14PTITKK136 pKa = 10.12QLLEE140 pKa = 4.22EE141 pKa = 4.45LNEE144 pKa = 4.5GEE146 pKa = 4.42DD147 pKa = 3.79EE148 pKa = 4.39KK149 pKa = 11.52SISTGYY155 pKa = 10.06HH156 pKa = 5.89AIEE159 pKa = 4.07FLLWGQDD166 pKa = 2.71ITAPAANLPGQRR178 pKa = 11.84PYY180 pKa = 11.33TDD182 pKa = 3.98FVDD185 pKa = 4.51GGTAEE190 pKa = 4.08NQDD193 pKa = 3.07RR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84AYY198 pKa = 10.98LNAAADD204 pKa = 4.11LLIDD208 pKa = 3.71HH209 pKa = 7.0LTYY212 pKa = 10.89LVDD215 pKa = 4.04LWAEE219 pKa = 4.15GGSYY223 pKa = 10.88RR224 pKa = 11.84NTFLALPEE232 pKa = 4.15EE233 pKa = 4.43EE234 pKa = 4.32ALQNIYY240 pKa = 10.77LGIITLCSSEE250 pKa = 4.48LPIEE254 pKa = 4.34RR255 pKa = 11.84MSVALEE261 pKa = 4.03NADD264 pKa = 3.71QEE266 pKa = 5.15DD267 pKa = 4.07EE268 pKa = 4.48HH269 pKa = 9.11SCFSDD274 pKa = 3.07NTHH277 pKa = 6.76RR278 pKa = 11.84DD279 pKa = 3.11VYY281 pKa = 11.27LNFAGIVNVYY291 pKa = 8.51EE292 pKa = 4.27GKK294 pKa = 10.58YY295 pKa = 10.85GNIDD299 pKa = 3.63GPSLADD305 pKa = 4.31LVKK308 pKa = 10.36QANNSKK314 pKa = 11.06YY315 pKa = 10.86NDD317 pKa = 3.32IEE319 pKa = 4.1NAIDD323 pKa = 3.58AAEE326 pKa = 4.52DD327 pKa = 4.39DD328 pKa = 4.25IEE330 pKa = 7.06AILTPFDD337 pKa = 3.86LAISGGPTSAEE348 pKa = 3.85GAKK351 pKa = 10.51VKK353 pKa = 10.27VAYY356 pKa = 7.41TQLIMLSQEE365 pKa = 4.12LLAGATASGITVNGIEE381 pKa = 4.04

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8MH56|A0A0M8MH56_9FLAO Oxidoreductase OS=Flavobacterium akiainvivens OX=1202724 GN=AM493_05220 PE=3 SV=1
MM1 pKa = 6.67FQKK4 pKa = 10.53PATRR8 pKa = 11.84LLFLLFLRR16 pKa = 11.84GQFKK20 pKa = 10.43LANRR24 pKa = 11.84GQFAPAKK31 pKa = 9.72GGQFQPARR39 pKa = 11.84GVFLNRR45 pKa = 11.84PRR47 pKa = 11.84GVSLHH52 pKa = 5.9WFVQNGLL59 pKa = 3.66

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3833

0

3833

1277701

46

3415

333.3

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.923 ± 0.043

0.773 ± 0.016

5.463 ± 0.028

6.193 ± 0.042

4.88 ± 0.028

6.986 ± 0.043

1.833 ± 0.02

6.842 ± 0.037

6.842 ± 0.065

8.996 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.021

5.716 ± 0.035

3.798 ± 0.029

3.653 ± 0.025

3.574 ± 0.029

5.764 ± 0.032

6.385 ± 0.061

6.676 ± 0.03

1.114 ± 0.016

4.323 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski