Daucus carota subsp. sativus (Carrot)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Apiales; Apiineae; Apiaceae; Apioideae; Scandiceae; Daucinae; <

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164WKA2|A0A164WKA2_DAUCS Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_020747 PE=3 SV=1
MM1 pKa = 7.58LMYY4 pKa = 10.49MMMSDD9 pKa = 4.76DD10 pKa = 4.02VKK12 pKa = 10.91CGYY15 pKa = 10.58DD16 pKa = 3.33RR17 pKa = 11.84DD18 pKa = 4.07NIEE21 pKa = 4.09YY22 pKa = 10.76VNVVDD27 pKa = 5.84SDD29 pKa = 3.79GQEE32 pKa = 4.11FFVSEE37 pKa = 3.99QLDD40 pKa = 3.92EE41 pKa = 4.55GEE43 pKa = 5.04KK44 pKa = 10.74LYY46 pKa = 11.15DD47 pKa = 5.08DD48 pKa = 4.38IGSMHH53 pKa = 6.85EE54 pKa = 4.31SSDD57 pKa = 3.73NLHH60 pKa = 7.29DD61 pKa = 4.31SGDD64 pKa = 3.93NIVDD68 pKa = 3.29GDD70 pKa = 4.17EE71 pKa = 5.18PISIPYY77 pKa = 10.01LNQKK81 pKa = 9.46FPNLQAGEE89 pKa = 4.11NLFSFKK95 pKa = 10.88AWFCNN100 pKa = 3.71

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A175YBG6|A0A175YBG6_DAUCS Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_031867 PE=4 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84GRR5 pKa = 11.84SSGALQAHH13 pKa = 7.59PGRR16 pKa = 11.84ARR18 pKa = 11.84AVRR21 pKa = 11.84SLGRR25 pKa = 11.84SSGALQALLGRR36 pKa = 11.84ARR38 pKa = 11.84GALFGALLGRR48 pKa = 11.84SSGASRR54 pKa = 11.84ALLAIMQALVFRR66 pKa = 11.84AGAEE70 pKa = 3.91

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31771

0

31771

12603374

16

5252

396.7

44.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.618 ± 0.013

1.842 ± 0.007

5.479 ± 0.009

6.424 ± 0.014

4.15 ± 0.009

6.468 ± 0.013

2.34 ± 0.006

5.435 ± 0.01

6.25 ± 0.012

9.494 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.006

4.653 ± 0.008

4.816 ± 0.016

3.677 ± 0.01

5.101 ± 0.01

8.942 ± 0.016

5.003 ± 0.008

6.663 ± 0.01

1.238 ± 0.005

2.939 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski