Beak and feather disease virus (BFDV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8CWE0|A0A3Q8CWE0_BFDV ATP-dependent helicase Rep OS=Beak and feather disease virus OX=77856 GN=Rep PE=3 SV=1
MM1 pKa = 7.59PSKK4 pKa = 10.45EE5 pKa = 3.95GSGCRR10 pKa = 11.84RR11 pKa = 11.84WCFTLNNPTDD21 pKa = 3.85GEE23 pKa = 4.42IEE25 pKa = 4.29FVRR28 pKa = 11.84SLGPDD33 pKa = 3.05EE34 pKa = 4.97FYY36 pKa = 10.98YY37 pKa = 11.01AIVGRR42 pKa = 11.84EE43 pKa = 4.0KK44 pKa = 11.1GEE46 pKa = 3.69QGTPHH51 pKa = 6.02LQGYY55 pKa = 7.3FHH57 pKa = 7.32FKK59 pKa = 9.42NKK61 pKa = 9.3KK62 pKa = 9.41RR63 pKa = 11.84LSALKK68 pKa = 10.2KK69 pKa = 9.75LLPRR73 pKa = 11.84AHH75 pKa = 6.9FEE77 pKa = 3.87RR78 pKa = 11.84AKK80 pKa = 11.12GSDD83 pKa = 3.4ADD85 pKa = 3.83NEE87 pKa = 4.57QYY89 pKa = 10.47CSKK92 pKa = 10.32EE93 pKa = 3.51GDD95 pKa = 3.64VILTLGIVARR105 pKa = 11.84DD106 pKa = 3.2GHH108 pKa = 6.21RR109 pKa = 11.84AFDD112 pKa = 3.73GAVAAVMSGRR122 pKa = 11.84KK123 pKa = 7.31MKK125 pKa = 10.31EE126 pKa = 3.65VARR129 pKa = 11.84EE130 pKa = 3.75FPEE133 pKa = 4.19VYY135 pKa = 9.73VRR137 pKa = 11.84HH138 pKa = 5.88GRR140 pKa = 11.84GLHH143 pKa = 5.46NLSLLVGSRR152 pKa = 11.84PRR154 pKa = 11.84DD155 pKa = 3.42FKK157 pKa = 11.18TEE159 pKa = 3.31VDD161 pKa = 4.22VIYY164 pKa = 10.46GPPGCGKK171 pKa = 10.27SRR173 pKa = 11.84WANEE177 pKa = 4.03QPGTKK182 pKa = 8.84YY183 pKa = 11.13YY184 pKa = 11.2KK185 pKa = 9.69MRR187 pKa = 11.84GEE189 pKa = 3.61WWDD192 pKa = 3.98GYY194 pKa = 11.01DD195 pKa = 4.14GEE197 pKa = 5.29DD198 pKa = 4.56VVILDD203 pKa = 5.37DD204 pKa = 4.22FYY206 pKa = 11.91GWLPYY211 pKa = 10.73CEE213 pKa = 4.27MLRR216 pKa = 11.84LCDD219 pKa = 4.27RR220 pKa = 11.84YY221 pKa = 9.16PHH223 pKa = 6.24KK224 pKa = 11.27VPVKK228 pKa = 8.87GAFVEE233 pKa = 5.24FTSKK237 pKa = 10.81RR238 pKa = 11.84IIITSNKK245 pKa = 9.26PPEE248 pKa = 3.83TWYY251 pKa = 10.87KK252 pKa = 10.18EE253 pKa = 4.29DD254 pKa = 4.32CDD256 pKa = 3.8PRR258 pKa = 11.84PLFRR262 pKa = 11.84RR263 pKa = 11.84FTRR266 pKa = 11.84VWWYY270 pKa = 10.97NVDD273 pKa = 3.17KK274 pKa = 11.07LEE276 pKa = 4.18QVRR279 pKa = 11.84PDD281 pKa = 4.09FLAHH285 pKa = 7.01PINYY289 pKa = 9.69

Molecular weight: 33.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8CWE0|A0A3Q8CWE0_BFDV ATP-dependent helicase Rep OS=Beak and feather disease virus OX=77856 GN=Rep PE=3 SV=1
MM1 pKa = 7.74WGTSNCACATFQIRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 8.12ARR20 pKa = 11.84PYY22 pKa = 8.65RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84HH26 pKa = 4.1IRR28 pKa = 11.84RR29 pKa = 11.84YY30 pKa = 8.2RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 3.92FRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84FSTNRR46 pKa = 11.84IYY48 pKa = 10.22TLRR51 pKa = 11.84LTRR54 pKa = 11.84QFQFKK59 pKa = 10.12INKK62 pKa = 6.25QTTSVGNLIFNADD75 pKa = 4.35YY76 pKa = 9.29ITFALDD82 pKa = 3.74DD83 pKa = 4.14FLQAVPNPHH92 pKa = 6.1TLNFEE97 pKa = 4.33DD98 pKa = 5.0YY99 pKa = 9.96RR100 pKa = 11.84IKK102 pKa = 10.03LAKK105 pKa = 9.51MEE107 pKa = 3.87MRR109 pKa = 11.84PTGGHH114 pKa = 4.99YY115 pKa = 9.08TVQSDD120 pKa = 4.41GFGHH124 pKa = 6.0TAVIQDD130 pKa = 3.41SRR132 pKa = 11.84ITRR135 pKa = 11.84FKK137 pKa = 8.59TTADD141 pKa = 3.34QTQDD145 pKa = 3.2PLAPFDD151 pKa = 4.02GAKK154 pKa = 9.61KK155 pKa = 8.9WFVSRR160 pKa = 11.84GFKK163 pKa = 10.32RR164 pKa = 11.84LLRR167 pKa = 11.84PKK169 pKa = 9.82PQITIEE175 pKa = 4.38DD176 pKa = 4.19LTTANQSAALWLNSARR192 pKa = 11.84TGWIPLQGGPNSAGTKK208 pKa = 8.03VRR210 pKa = 11.84HH211 pKa = 5.36YY212 pKa = 10.73GIAFSFPQPEE222 pKa = 3.77QTITYY227 pKa = 6.7VTKK230 pKa = 9.7LTLYY234 pKa = 9.08VQFRR238 pKa = 11.84QFAPNNPSTT247 pKa = 3.82

Molecular weight: 28.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

536

247

289

268.0

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.97 ± 0.844

1.679 ± 0.557

5.41 ± 0.873

4.664 ± 1.952

6.157 ± 0.725

7.276 ± 1.29

2.612 ± 0.117

4.478 ± 0.763

5.97 ± 0.972

6.716 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.493 ± 0.178

3.918 ± 0.603

6.157 ± 0.313

3.918 ± 1.641

10.448 ± 1.348

4.291 ± 0.104

6.716 ± 2.442

5.224 ± 1.272

2.239 ± 0.397

4.664 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski