Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Aeromonas hydrophila subsp. hydrophila

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0KLV0|A0KLV0_AERHH Ferrous iron transport protein B OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=feoB PE=3 SV=1
MM1 pKa = 7.73QSFNWVDD8 pKa = 2.91IDD10 pKa = 4.27GIRR13 pKa = 11.84YY14 pKa = 8.99SWDD17 pKa = 3.1GQGSITCTGQFGYY30 pKa = 9.94HH31 pKa = 5.31ATMAGDD37 pKa = 4.83RR38 pKa = 11.84IDD40 pKa = 5.12ALDD43 pKa = 4.36LEE45 pKa = 4.89SDD47 pKa = 3.86DD48 pKa = 5.39GEE50 pKa = 4.58VAGSLNVDD58 pKa = 4.49FAAATISFTPAVDD71 pKa = 3.44TAPPHH76 pKa = 5.53MMVLVGHH83 pKa = 5.9EE84 pKa = 4.22QGQQEE89 pKa = 4.46YY90 pKa = 8.28TLQSLSDD97 pKa = 3.49VCLQDD102 pKa = 5.35LLGHH106 pKa = 6.52SDD108 pKa = 4.72NIDD111 pKa = 3.58CAPLVTTTMTEE122 pKa = 3.97PVAPLAGHH130 pKa = 5.37VLSWDD135 pKa = 3.57SFHH138 pKa = 7.78FDD140 pKa = 4.09DD141 pKa = 6.47DD142 pKa = 3.98VLLGSRR148 pKa = 11.84SATPLAATHH157 pKa = 6.06QGLPGGEE164 pKa = 3.39ISINIDD170 pKa = 2.97MTNQVLDD177 pKa = 4.43QIPPLHH183 pKa = 7.26DD184 pKa = 2.86II185 pKa = 4.52

Molecular weight: 19.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0KR13|A0KR13_AERHH Histidine kinase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=AHA_0032 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84ARR41 pKa = 11.84LVVV44 pKa = 3.3

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4121

0

4121

1379394

15

5047

334.7

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.573 ± 0.049

1.133 ± 0.015

5.114 ± 0.036

5.831 ± 0.037

3.619 ± 0.025

7.971 ± 0.042

2.342 ± 0.021

5.023 ± 0.031

3.8 ± 0.03

12.087 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.023

3.039 ± 0.03

4.745 ± 0.029

4.879 ± 0.035

5.901 ± 0.038

5.624 ± 0.029

4.819 ± 0.049

6.827 ± 0.029

1.472 ± 0.018

2.61 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski