Clostridium cylindrosporum DSM 605

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium cylindrosporum

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8G098|A0A0J8G098_CLOCY Translation initiation factor IF-1 OS=Clostridium cylindrosporum DSM 605 OX=1121307 GN=infA PE=3 SV=1
MM1 pKa = 8.01DD2 pKa = 4.93KK3 pKa = 10.58YY4 pKa = 11.53VCIVCDD10 pKa = 3.47YY11 pKa = 11.17VYY13 pKa = 10.78DD14 pKa = 4.13PEE16 pKa = 6.42FGDD19 pKa = 3.91PDD21 pKa = 3.83GGIAPGTKK29 pKa = 9.95FEE31 pKa = 6.11DD32 pKa = 4.08IPDD35 pKa = 3.66DD36 pKa = 4.07WVCPLCGVIKK46 pKa = 10.6DD47 pKa = 3.93DD48 pKa = 4.1FKK50 pKa = 11.41KK51 pKa = 11.14VEE53 pKa = 3.86

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J8D8U8|A0A0J8D8U8_CLOCY DNA polymerase III subunit gamma/tau OS=Clostridium cylindrosporum DSM 605 OX=1121307 GN=dnaX PE=3 SV=1
MM1 pKa = 7.52FGPFGFVPFGFGPIVPIIPIPINRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84FPIGIGVILL40 pKa = 4.22

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

789336

40

1782

301.2

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.683 ± 0.055

1.1 ± 0.016

5.604 ± 0.038

7.439 ± 0.053

4.227 ± 0.035

6.596 ± 0.037

1.394 ± 0.02

9.946 ± 0.058

8.864 ± 0.049

8.929 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.021

5.756 ± 0.042

2.811 ± 0.024

2.129 ± 0.022

3.603 ± 0.033

7.093 ± 0.047

4.948 ± 0.033

6.865 ± 0.039

0.577 ± 0.012

3.925 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski