Sewage-associated circular DNA virus-27

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGZ0|A0A0B4UGZ0_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-27 OX=1592094 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.26RR3 pKa = 11.84RR4 pKa = 11.84QGIYY8 pKa = 9.37WLLTIPFHH16 pKa = 6.43EE17 pKa = 4.47WTPYY21 pKa = 10.36LPPGVSWIRR30 pKa = 11.84GQLEE34 pKa = 4.14SGSEE38 pKa = 3.74SGYY41 pKa = 9.4LHH43 pKa = 5.88WQIVVALTTKK53 pKa = 10.49CSLRR57 pKa = 11.84GIKK60 pKa = 10.35GLFGDD65 pKa = 4.22STHH68 pKa = 7.05GEE70 pKa = 3.83LSRR73 pKa = 11.84TNRR76 pKa = 11.84ASVYY80 pKa = 7.78VWKK83 pKa = 10.64EE84 pKa = 3.56EE85 pKa = 3.81TRR87 pKa = 11.84IEE89 pKa = 4.15GTQFEE94 pKa = 4.62LGEE97 pKa = 4.23RR98 pKa = 11.84PFKK101 pKa = 10.85RR102 pKa = 11.84NCSKK106 pKa = 10.71DD107 pKa = 2.87WEE109 pKa = 4.66QIWEE113 pKa = 4.2CAQRR117 pKa = 11.84GDD119 pKa = 4.76LMSIPADD126 pKa = 3.44VRR128 pKa = 11.84VHH130 pKa = 6.39SYY132 pKa = 8.51RR133 pKa = 11.84TLRR136 pKa = 11.84AISADD141 pKa = 3.52YY142 pKa = 10.83SKK144 pKa = 10.17PLPMVRR150 pKa = 11.84DD151 pKa = 3.09IHH153 pKa = 7.77VYY155 pKa = 9.98IGPTATGKK163 pKa = 9.78SRR165 pKa = 11.84RR166 pKa = 11.84AWDD169 pKa = 3.51EE170 pKa = 3.81AGMEE174 pKa = 4.72AYY176 pKa = 10.5CKK178 pKa = 10.64DD179 pKa = 3.72PNSKK183 pKa = 9.69FWCGYY188 pKa = 9.01SGQPNIVLDD197 pKa = 4.37EE198 pKa = 3.89YY199 pKa = 10.81RR200 pKa = 11.84GRR202 pKa = 11.84IDD204 pKa = 3.65VSHH207 pKa = 7.42LLRR210 pKa = 11.84WFDD213 pKa = 3.73RR214 pKa = 11.84YY215 pKa = 9.57PVNVEE220 pKa = 3.73IKK222 pKa = 9.78GSSVPLCAEE231 pKa = 4.28SFWITTNLDD240 pKa = 2.7VDD242 pKa = 3.33QWYY245 pKa = 9.3PDD247 pKa = 3.58LDD249 pKa = 3.8ATTLEE254 pKa = 3.98ALRR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84LKK261 pKa = 9.11ITYY264 pKa = 8.88FRR266 pKa = 5.2

Molecular weight: 30.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGZ0|A0A0B4UGZ0_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-27 OX=1592094 PE=4 SV=1
MM1 pKa = 7.63TIRR4 pKa = 11.84HH5 pKa = 5.88EE6 pKa = 4.16LAGLAGGTLGFIHH19 pKa = 7.74DD20 pKa = 4.14NTRR23 pKa = 11.84GAIKK27 pKa = 10.26GYY29 pKa = 10.32YY30 pKa = 9.38LGKK33 pKa = 10.02QLSKK37 pKa = 11.44SMAPIPKK44 pKa = 9.38KK45 pKa = 10.3RR46 pKa = 11.84RR47 pKa = 11.84NSYY50 pKa = 7.95STPSTRR56 pKa = 11.84GTITNGILSRR66 pKa = 11.84RR67 pKa = 11.84SSNPFDD73 pKa = 4.11RR74 pKa = 11.84RR75 pKa = 11.84LSDD78 pKa = 3.15VSMRR82 pKa = 11.84SAKK85 pKa = 8.64GTYY88 pKa = 9.41RR89 pKa = 11.84AFTGRR94 pKa = 11.84GTGSASASGYY104 pKa = 10.41RR105 pKa = 11.84SISGNPAAIKK115 pKa = 9.46KK116 pKa = 9.25RR117 pKa = 11.84KK118 pKa = 9.26RR119 pKa = 11.84KK120 pKa = 9.91GVVLKK125 pKa = 10.42KK126 pKa = 9.99KK127 pKa = 10.34RR128 pKa = 11.84RR129 pKa = 11.84VKK131 pKa = 10.74VSGKK135 pKa = 9.65FKK137 pKa = 10.7RR138 pKa = 11.84KK139 pKa = 5.99VHH141 pKa = 6.14KK142 pKa = 10.82ALDD145 pKa = 3.38EE146 pKa = 4.67KK147 pKa = 10.94IYY149 pKa = 10.46GKK151 pKa = 9.57HH152 pKa = 6.0LKK154 pKa = 9.07VHH156 pKa = 6.31YY157 pKa = 10.02DD158 pKa = 3.43RR159 pKa = 11.84LPATGSYY166 pKa = 8.48QQSIKK171 pKa = 11.08DD172 pKa = 3.74FGHH175 pKa = 7.21LFTPLRR181 pKa = 11.84MIAAADD187 pKa = 3.48VLFNKK192 pKa = 10.17APVVEE197 pKa = 6.26FPATTDD203 pKa = 3.32IKK205 pKa = 10.42WNNSFIRR212 pKa = 11.84QDD214 pKa = 3.47YY215 pKa = 10.04VINSWASFEE224 pKa = 5.26LKK226 pKa = 10.29NCSQRR231 pKa = 11.84TYY233 pKa = 8.77TIKK236 pKa = 10.04MYY238 pKa = 10.28EE239 pKa = 4.24CKK241 pKa = 9.95PLNKK245 pKa = 9.94AVLPVTNDD253 pKa = 3.47AYY255 pKa = 10.89ADD257 pKa = 4.19WIRR260 pKa = 11.84GMLAMQGLQTNPASNTPNTLYY281 pKa = 10.6SVPFDD286 pKa = 3.34SPQFNQCWKK295 pKa = 11.08AEE297 pKa = 4.06VTTCILEE304 pKa = 4.38PGQSNTFFVQGPNQQSIDD322 pKa = 3.35WQKK325 pKa = 10.86YY326 pKa = 6.44VYY328 pKa = 9.6EE329 pKa = 4.12NSGFVDD335 pKa = 3.23VMNEE339 pKa = 3.54FATWSRR345 pKa = 11.84DD346 pKa = 3.39VFFVYY351 pKa = 10.96YY352 pKa = 9.84EE353 pKa = 4.28DD354 pKa = 5.88LVTTDD359 pKa = 3.39GNNVGRR365 pKa = 11.84IVSGPVTGGGGVAIEE380 pKa = 4.1FKK382 pKa = 10.92EE383 pKa = 4.14FFKK386 pKa = 11.58LEE388 pKa = 4.15VPASAGFTYY397 pKa = 9.98PASTAAGVKK406 pKa = 9.48QQLDD410 pKa = 3.39NKK412 pKa = 9.84LPTKK416 pKa = 10.04IISVFPQIVVGNVVDD431 pKa = 3.98VAEE434 pKa = 4.5EE435 pKa = 4.07NPAVSTNPYY444 pKa = 10.12DD445 pKa = 3.33

Molecular weight: 49.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

711

266

445

355.5

40.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.47 ± 0.854

1.406 ± 0.458

4.923 ± 0.386

4.641 ± 1.146

4.36 ± 0.73

7.595 ± 0.244

1.688 ± 0.306

5.485 ± 0.286

6.47 ± 1.259

6.61 ± 0.895

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.688 ± 0.099

4.501 ± 1.211

5.345 ± 0.044

3.516 ± 0.274

6.892 ± 1.149

7.736 ± 0.117

6.61 ± 0.321

7.032 ± 0.751

2.532 ± 1.068

4.501 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski