Amino acid dipepetide frequency for Dracunculus medinensis (Guinea worm)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.293AlaAla: 4.293 ± 0.05	1.19AlaCys: 1.19 ± 0.018	2.997AlaAsp: 2.997 ± 0.028	3.707AlaGlu: 3.707 ± 0.038	2.762AlaPhe: 2.762 ± 0.03	2.748AlaGly: 2.748 ± 0.033	1.265AlaHis: 1.265 ± 0.019	4.626AlaIle: 4.626 ± 0.038	3.755AlaLys: 3.755 ± 0.042	5.658AlaLeu: 5.658 ± 0.045	1.447AlaMet: 1.447 ± 0.019	3.093AlaAsn: 3.093 ± 0.035	2.147AlaPro: 2.147 ± 0.025	2.124AlaGln: 2.124 ± 0.03	2.886AlaArg: 2.886 ± 0.031	4.531AlaSer: 4.531 ± 0.036	3.147AlaThr: 3.147 ± 0.033	3.933AlaVal: 3.933 ± 0.034	0.576AlaTrp: 0.576 ± 0.013	1.863AlaTyr: 1.863 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	1.418CysAla: 1.418 ± 0.022	0.649CysCys: 0.649 ± 0.017	1.402CysAsp: 1.402 ± 0.029	1.44CysGlu: 1.44 ± 0.025	1.167CysPhe: 1.167 ± 0.018	1.513CysGly: 1.513 ± 0.025	0.607CysHis: 0.607 ± 0.016	1.687CysIle: 1.687 ± 0.027	1.387CysLys: 1.387 ± 0.027	2.068CysLeu: 2.068 ± 0.028	0.511CysMet: 0.511 ± 0.011	1.209CysAsn: 1.209 ± 0.02	1.147CysPro: 1.147 ± 0.027	0.889CysGln: 0.889 ± 0.019	1.344CysArg: 1.344 ± 0.023	2.03CysSer: 2.03 ± 0.027	1.126CysThr: 1.126 ± 0.019	1.3CysVal: 1.3 ± 0.024	0.248CysTrp: 0.248 ± 0.008	0.719CysTyr: 0.719 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.973AspAla: 2.973 ± 0.031	1.191AspCys: 1.191 ± 0.025	3.635AspAsp: 3.635 ± 0.043	4.467AspGlu: 4.467 ± 0.041	2.733AspPhe: 2.733 ± 0.026	3.249AspGly: 3.249 ± 0.045	1.134AspHis: 1.134 ± 0.015	3.876AspIle: 3.876 ± 0.035	2.973AspLys: 2.973 ± 0.029	4.94AspLeu: 4.94 ± 0.043	1.138AspMet: 1.138 ± 0.017	2.568AspAsn: 2.568 ± 0.028	2.057AspPro: 2.057 ± 0.025	1.786AspGln: 1.786 ± 0.023	2.746AspArg: 2.746 ± 0.028	4.145AspSer: 4.145 ± 0.035	2.049AspThr: 2.049 ± 0.024	3.026AspVal: 3.026 ± 0.027	0.645AspTrp: 0.645 ± 0.013	1.936AspTyr: 1.936 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	3.569GluAla: 3.569 ± 0.034	1.393GluCys: 1.393 ± 0.029	3.047GluAsp: 3.047 ± 0.032	4.976GluGlu: 4.976 ± 0.064	2.662GluPhe: 2.662 ± 0.027	2.543GluGly: 2.543 ± 0.026	1.327GluHis: 1.327 ± 0.02	5.137GluIle: 5.137 ± 0.046	5.406GluLys: 5.406 ± 0.053	5.693GluLeu: 5.693 ± 0.045	1.811GluMet: 1.811 ± 0.022	4.284GluAsn: 4.284 ± 0.038	1.948GluPro: 1.948 ± 0.029	2.614GluGln: 2.614 ± 0.033	3.798GluArg: 3.798 ± 0.041	4.209GluSer: 4.209 ± 0.037	3.127GluThr: 3.127 ± 0.03	3.052GluVal: 3.052 ± 0.033	0.766GluTrp: 0.766 ± 0.013	2.12GluTyr: 2.12 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.013PheAla: 3.013 ± 0.032	1.335PheCys: 1.335 ± 0.023	2.944PheAsp: 2.944 ± 0.032	2.771PheGlu: 2.771 ± 0.026	2.901PhePhe: 2.901 ± 0.037	2.782PheGly: 2.782 ± 0.033	1.276PheHis: 1.276 ± 0.017	3.893PheIle: 3.893 ± 0.036	2.435PheLys: 2.435 ± 0.026	4.871PheLeu: 4.871 ± 0.039	1.174PheMet: 1.174 ± 0.019	2.373PheAsn: 2.373 ± 0.026	1.911PhePro: 1.911 ± 0.022	1.658PheGln: 1.658 ± 0.022	2.438PheArg: 2.438 ± 0.025	4.142PheSer: 4.142 ± 0.033	2.34PheThr: 2.34 ± 0.028	2.965PheVal: 2.965 ± 0.034	0.581PheTrp: 0.581 ± 0.014	1.889PheTyr: 1.889 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	2.743GlyAla: 2.743 ± 0.035	1.185GlyCys: 1.185 ± 0.021	2.71GlyAsp: 2.71 ± 0.027	3.053GlyGlu: 3.053 ± 0.037	2.518GlyPhe: 2.518 ± 0.026	3.021GlyGly: 3.021 ± 0.05	1.227GlyHis: 1.227 ± 0.02	3.798GlyIle: 3.798 ± 0.036	3.267GlyLys: 3.267 ± 0.034	4.188GlyLeu: 4.188 ± 0.048	1.145GlyMet: 1.145 ± 0.018	2.658GlyAsn: 2.658 ± 0.031	1.919GlyPro: 1.919 ± 0.05	1.868GlyGln: 1.868 ± 0.024	2.912GlyArg: 2.912 ± 0.034	3.957GlySer: 3.957 ± 0.036	2.641GlyThr: 2.641 ± 0.028	2.736GlyVal: 2.736 ± 0.028	0.613GlyTrp: 0.613 ± 0.015	1.897GlyTyr: 1.897 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	1.145HisAla: 1.145 ± 0.017	0.663HisCys: 0.663 ± 0.013	1.021HisAsp: 1.021 ± 0.017	1.311HisGlu: 1.311 ± 0.019	1.3HisPhe: 1.3 ± 0.017	1.203HisGly: 1.203 ± 0.018	0.674HisHis: 0.674 ± 0.017	1.664HisIle: 1.664 ± 0.022	1.18HisLys: 1.18 ± 0.017	2.418HisLeu: 2.418 ± 0.024	0.565HisMet: 0.565 ± 0.013	1.081HisAsn: 1.081 ± 0.018	1.076HisPro: 1.076 ± 0.017	0.91HisGln: 0.91 ± 0.016	1.393HisArg: 1.393 ± 0.02	1.975HisSer: 1.975 ± 0.022	0.96HisThr: 0.96 ± 0.017	1.181HisVal: 1.181 ± 0.016	0.332HisTrp: 0.332 ± 0.01	0.896HisTyr: 0.896 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.792IleAla: 4.792 ± 0.04	1.963IleCys: 1.963 ± 0.026	4.329IleAsp: 4.329 ± 0.034	4.548IleGlu: 4.548 ± 0.042	4.173IlePhe: 4.173 ± 0.041	3.818IleGly: 3.818 ± 0.036	1.725IleHis: 1.725 ± 0.021	5.569IleIle: 5.569 ± 0.048	4.145IleLys: 4.145 ± 0.033	6.828IleLeu: 6.828 ± 0.054	1.59IleMet: 1.59 ± 0.021	3.985IleAsn: 3.985 ± 0.031	3.09IlePro: 3.09 ± 0.033	2.49IleGln: 2.49 ± 0.026	3.91IleArg: 3.91 ± 0.035	6.461IleSer: 6.461 ± 0.047	3.691IleThr: 3.691 ± 0.033	4.328IleVal: 4.328 ± 0.038	0.833IleTrp: 0.833 ± 0.016	2.643IleTyr: 2.643 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.459LysAla: 3.459 ± 0.036	1.598LysCys: 1.598 ± 0.03	3.021LysAsp: 3.021 ± 0.035	4.367LysGlu: 4.367 ± 0.046	2.89LysPhe: 2.89 ± 0.03	2.639LysGly: 2.639 ± 0.032	1.308LysHis: 1.308 ± 0.021	5.025LysIle: 5.025 ± 0.043	5.242LysLys: 5.242 ± 0.062	5.832LysLeu: 5.832 ± 0.043	1.679LysMet: 1.679 ± 0.022	3.981LysAsn: 3.981 ± 0.036	2.208LysPro: 2.208 ± 0.029	2.461LysGln: 2.461 ± 0.026	3.876LysArg: 3.876 ± 0.035	4.51LysSer: 4.51 ± 0.033	3.109LysThr: 3.109 ± 0.029	3.146LysVal: 3.146 ± 0.026	0.782LysTrp: 0.782 ± 0.015	2.337LysTyr: 2.337 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	5.717LeuAla: 5.717 ± 0.048	2.218LeuCys: 2.218 ± 0.027	4.551LeuAsp: 4.551 ± 0.04	5.545LeuGlu: 5.545 ± 0.052	4.692LeuPhe: 4.692 ± 0.048	3.843LeuGly: 3.843 ± 0.03	2.3LeuHis: 2.3 ± 0.028	6.876LeuIle: 6.876 ± 0.052	6.211LeuLys: 6.211 ± 0.044	9.531LeuLeu: 9.531 ± 0.077	2.31LeuMet: 2.31 ± 0.023	5.236LeuAsn: 5.236 ± 0.039	4.4LeuPro: 4.4 ± 0.046	3.951LeuGln: 3.951 ± 0.033	5.286LeuArg: 5.286 ± 0.042	7.953LeuSer: 7.953 ± 0.058	4.593LeuThr: 4.593 ± 0.039	4.746LeuVal: 4.746 ± 0.038	1.035LeuTrp: 1.035 ± 0.019	3.069LeuTyr: 3.069 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	1.621MetAla: 1.621 ± 0.022	0.475MetCys: 0.475 ± 0.011	1.387MetAsp: 1.387 ± 0.019	1.669MetGlu: 1.669 ± 0.022	0.974MetPhe: 0.974 ± 0.019	1.043MetGly: 1.043 ± 0.017	0.59MetHis: 0.59 ± 0.013	1.609MetIle: 1.609 ± 0.02	1.793MetLys: 1.793 ± 0.023	2.144MetLeu: 2.144 ± 0.023	0.605MetMet: 0.605 ± 0.014	1.476MetAsn: 1.476 ± 0.02	1.043MetPro: 1.043 ± 0.016	1.098MetGln: 1.098 ± 0.018	1.391MetArg: 1.391 ± 0.019	1.628MetSer: 1.628 ± 0.022	1.081MetThr: 1.081 ± 0.02	1.456MetVal: 1.456 ± 0.019	0.224MetTrp: 0.224 ± 0.007	0.616MetTyr: 0.616 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.308AsnAla: 3.308 ± 0.028	1.35AsnCys: 1.35 ± 0.023	3.344AsnAsp: 3.344 ± 0.03	4.112AsnGlu: 4.112 ± 0.041	2.864AsnPhe: 2.864 ± 0.025	3.25AsnGly: 3.25 ± 0.029	1.161AsnHis: 1.161 ± 0.018	4.326AsnIle: 4.326 ± 0.037	2.975AsnLys: 2.975 ± 0.029	4.992AsnLeu: 4.992 ± 0.032	1.294AsnMet: 1.294 ± 0.02	3.182AsnAsn: 3.182 ± 0.04	2.077AsnPro: 2.077 ± 0.025	1.979AsnGln: 1.979 ± 0.027	2.865AsnArg: 2.865 ± 0.029	4.557AsnSer: 4.557 ± 0.04	2.263AsnThr: 2.263 ± 0.021	3.057AsnVal: 3.057 ± 0.03	0.588AsnTrp: 0.588 ± 0.013	1.963AsnTyr: 1.963 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	2.046ProAla: 2.046 ± 0.025	0.793ProCys: 0.793 ± 0.018	1.983ProAsp: 1.983 ± 0.024	2.44ProGlu: 2.44 ± 0.027	2.047ProPhe: 2.047 ± 0.025	2.257ProGly: 2.257 ± 0.083	0.919ProHis: 0.919 ± 0.017	2.816ProIle: 2.816 ± 0.031	2.285ProLys: 2.285 ± 0.026	3.841ProLeu: 3.841 ± 0.035	0.844ProMet: 0.844 ± 0.016	2.175ProAsn: 2.175 ± 0.027	2.746ProPro: 2.746 ± 0.055	1.582ProGln: 1.582 ± 0.021	1.913ProArg: 1.913 ± 0.023	3.836ProSer: 3.836 ± 0.039	2.249ProThr: 2.249 ± 0.037	2.523ProVal: 2.523 ± 0.03	0.405ProTrp: 0.405 ± 0.01	1.49ProTyr: 1.49 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	2.011GlnAla: 2.011 ± 0.026	1.024GlnCys: 1.024 ± 0.02	1.278GlnAsp: 1.278 ± 0.015	2.066GlnGlu: 2.066 ± 0.027	1.825GlnPhe: 1.825 ± 0.023	1.405GlnGly: 1.405 ± 0.024	0.856GlnHis: 0.856 ± 0.014	3.009GlnIle: 3.009 ± 0.029	2.676GlnLys: 2.676 ± 0.028	4.098GlnLeu: 4.098 ± 0.041	1.138GlnMet: 1.138 ± 0.017	2.212GlnAsn: 2.212 ± 0.026	1.573GlnPro: 1.573 ± 0.022	2.375GlnGln: 2.375 ± 0.052	2.378GlnArg: 2.378 ± 0.029	2.81GlnSer: 2.81 ± 0.031	1.844GlnThr: 1.844 ± 0.025	1.903GlnVal: 1.903 ± 0.021	0.504GlnTrp: 0.504 ± 0.012	1.296GlnTyr: 1.296 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	2.789ArgAla: 2.789 ± 0.026	1.344ArgCys: 1.344 ± 0.023	2.555ArgAsp: 2.555 ± 0.031	3.235ArgGlu: 3.235 ± 0.038	2.72ArgPhe: 2.72 ± 0.027	2.401ArgGly: 2.401 ± 0.034	1.29ArgHis: 1.29 ± 0.018	4.263ArgIle: 4.263 ± 0.037	4.089ArgLys: 4.089 ± 0.04	5.348ArgLeu: 5.348 ± 0.039	1.352ArgMet: 1.352 ± 0.019	3.176ArgAsn: 3.176 ± 0.028	2.118ArgPro: 2.118 ± 0.024	2.24ArgGln: 2.24 ± 0.028	3.841ArgArg: 3.841 ± 0.043	4.226ArgSer: 4.226 ± 0.04	2.668ArgThr: 2.668 ± 0.03	2.547ArgVal: 2.547 ± 0.027	0.631ArgTrp: 0.631 ± 0.015	1.911ArgTyr: 1.911 ± 0.023	0.0ArgXaa: 0.0 ± 0.0
Ser	4.721SerAla: 4.721 ± 0.036	1.799SerCys: 1.799 ± 0.027	4.507SerAsp: 4.507 ± 0.037	4.664SerGlu: 4.664 ± 0.043	4.027SerPhe: 4.027 ± 0.038	4.279SerGly: 4.279 ± 0.04	1.75SerHis: 1.75 ± 0.025	5.564SerIle: 5.564 ± 0.043	4.681SerLys: 4.681 ± 0.041	7.753SerLeu: 7.753 ± 0.053	1.78SerMet: 1.78 ± 0.022	4.568SerAsn: 4.568 ± 0.04	3.354SerPro: 3.354 ± 0.042	2.843SerGln: 2.843 ± 0.031	3.977SerArg: 3.977 ± 0.038	8.357SerSer: 8.357 ± 0.075	4.544SerThr: 4.544 ± 0.044	4.844SerVal: 4.844 ± 0.036	0.823SerTrp: 0.823 ± 0.015	2.401SerTyr: 2.401 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	3.164ThrAla: 3.164 ± 0.032	1.17ThrCys: 1.17 ± 0.025	2.593ThrAsp: 2.593 ± 0.027	2.791ThrGlu: 2.791 ± 0.028	2.306ThrPhe: 2.306 ± 0.028	2.661ThrGly: 2.661 ± 0.031	1.0ThrHis: 1.0 ± 0.015	3.868ThrIle: 3.868 ± 0.035	2.921ThrLys: 2.921 ± 0.028	4.377ThrLeu: 4.377 ± 0.036	1.155ThrMet: 1.155 ± 0.018	2.816ThrAsn: 2.816 ± 0.03	2.089ThrPro: 2.089 ± 0.028	1.505ThrGln: 1.505 ± 0.022	2.215ThrArg: 2.215 ± 0.025	4.21ThrSer: 4.21 ± 0.037	3.103ThrThr: 3.103 ± 0.045	3.358ThrVal: 3.358 ± 0.032	0.514ThrTrp: 0.514 ± 0.013	1.477ThrTyr: 1.477 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	3.515ValAla: 3.515 ± 0.027	1.326ValCys: 1.326 ± 0.022	3.458ValAsp: 3.458 ± 0.031	3.686ValGlu: 3.686 ± 0.035	2.655ValPhe: 2.655 ± 0.031	2.874ValGly: 2.874 ± 0.031	1.403ValHis: 1.403 ± 0.022	4.086ValIle: 4.086 ± 0.038	3.319ValLys: 3.319 ± 0.032	5.076ValLeu: 5.076 ± 0.041	1.249ValMet: 1.249 ± 0.019	2.982ValAsn: 2.982 ± 0.031	2.514ValPro: 2.514 ± 0.027	2.162ValGln: 2.162 ± 0.026	2.859ValArg: 2.859 ± 0.029	4.137ValSer: 4.137 ± 0.038	2.724ValThr: 2.724 ± 0.027	3.633ValVal: 3.633 ± 0.079	0.613ValTrp: 0.613 ± 0.013	1.727ValTyr: 1.727 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	0.576TrpAla: 0.576 ± 0.015	0.232TrpCys: 0.232 ± 0.007	0.564TrpAsp: 0.564 ± 0.013	0.558TrpGlu: 0.558 ± 0.013	0.548TrpPhe: 0.548 ± 0.012	0.435TrpGly: 0.435 ± 0.012	0.277TrpHis: 0.277 ± 0.009	0.889TrpIle: 0.889 ± 0.016	0.857TrpLys: 0.857 ± 0.014	1.158TrpLeu: 1.158 ± 0.019	0.327TrpMet: 0.327 ± 0.009	0.768TrpAsn: 0.768 ± 0.015	0.44TrpPro: 0.44 ± 0.011	0.475TrpGln: 0.475 ± 0.011	0.674TrpArg: 0.674 ± 0.014	0.901TrpSer: 0.901 ± 0.018	0.604TrpThr: 0.604 ± 0.013	0.482TrpVal: 0.482 ± 0.012	0.174TrpTrp: 0.174 ± 0.007	0.386TrpTyr: 0.386 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.875TyrAla: 1.875 ± 0.025	0.964TyrCys: 0.964 ± 0.018	1.943TyrAsp: 1.943 ± 0.024	2.078TyrGlu: 2.078 ± 0.024	1.927TyrPhe: 1.927 ± 0.027	2.09TyrGly: 2.09 ± 0.024	0.859TyrHis: 0.859 ± 0.017	2.332TyrIle: 2.332 ± 0.026	1.874TyrLys: 1.874 ± 0.026	3.248TyrLeu: 3.248 ± 0.032	0.808TyrMet: 0.808 ± 0.017	1.684TyrAsn: 1.684 ± 0.018	1.334TyrPro: 1.334 ± 0.021	1.268TyrGln: 1.268 ± 0.018	2.042TyrArg: 2.042 ± 0.025	2.661TyrSer: 2.661 ± 0.026	1.43TyrThr: 1.43 ± 0.021	1.778TyrVal: 1.778 ± 0.023	0.439TyrTrp: 0.439 ± 0.013	1.36TyrTyr: 1.36 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.005

Statistics based on 10868 proteins (4070558 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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