Aeromonas phage LAh1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Ahphunavirus; unclassified Ahphunavirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513ZZ38|A0A513ZZ38_9CAUD Uncharacterized protein OS=Aeromonas phage LAh1 OX=2591024 GN=LAh1_13 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 6.26DD3 pKa = 4.17EE4 pKa = 6.43LIDD7 pKa = 4.89DD8 pKa = 4.46LHH10 pKa = 6.67DD11 pKa = 3.48TSAADD16 pKa = 3.91DD17 pKa = 4.32AADD20 pKa = 3.52KK21 pKa = 11.54AFMEE25 pKa = 4.65RR26 pKa = 11.84LRR28 pKa = 11.84AMSNEE33 pKa = 3.85DD34 pKa = 3.74LAKK37 pKa = 10.89LLLNEE42 pKa = 4.46SLISLARR49 pKa = 11.84TINKK53 pKa = 8.82GWATAADD60 pKa = 4.16LNAARR65 pKa = 11.84QFLKK69 pKa = 11.02DD70 pKa = 3.43NDD72 pKa = 3.56IGIVPTRR79 pKa = 11.84TNAAGKK85 pKa = 10.15LKK87 pKa = 10.34EE88 pKa = 3.96ALQARR93 pKa = 11.84SASSQQAPGVIPVEE107 pKa = 4.2EE108 pKa = 4.53LDD110 pKa = 5.2KK111 pKa = 11.62LDD113 pKa = 3.53IDD115 pKa = 5.24DD116 pKa = 6.59FIRR119 pKa = 11.84LQQ121 pKa = 3.37

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513ZZ47|A0A513ZZ47_9CAUD Uncharacterized protein OS=Aeromonas phage LAh1 OX=2591024 GN=LAh1_19 PE=4 SV=1
MM1 pKa = 7.62PSKK4 pKa = 11.0AEE6 pKa = 3.88YY7 pKa = 9.83LQQLTRR13 pKa = 11.84YY14 pKa = 8.03KK15 pKa = 10.57QSAKK19 pKa = 10.77LEE21 pKa = 4.22AKK23 pKa = 9.8VQYY26 pKa = 10.8LSDD29 pKa = 3.69KK30 pKa = 9.01ATKK33 pKa = 8.86ATAEE37 pKa = 4.21GAACRR42 pKa = 11.84QKK44 pKa = 10.23RR45 pKa = 11.84KK46 pKa = 9.9QMATSQDD53 pKa = 3.08AKK55 pKa = 10.56RR56 pKa = 11.84PRR58 pKa = 11.84QTGFSTKK65 pKa = 8.95VRR67 pKa = 11.84AGSKK71 pKa = 9.56TDD73 pKa = 3.22VSINKK78 pKa = 9.45YY79 pKa = 10.19YY80 pKa = 10.09KK81 pKa = 10.4AKK83 pKa = 10.74HH84 pKa = 6.0GDD86 pKa = 3.2NLPRR90 pKa = 11.84VDD92 pKa = 4.63RR93 pKa = 11.84QPCLMM98 pKa = 5.02

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12987

59

1252

288.6

31.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.411 ± 0.658

1.209 ± 0.191

5.937 ± 0.212

5.944 ± 0.255

3.303 ± 0.233

7.908 ± 0.25

2.118 ± 0.193

4.181 ± 0.254

5.737 ± 0.288

8.793 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.095 ± 0.177

3.973 ± 0.236

3.912 ± 0.239

4.728 ± 0.374

5.767 ± 0.237

5.344 ± 0.203

5.752 ± 0.297

6.283 ± 0.236

1.409 ± 0.144

3.196 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski