Mycobacterium phage Blexus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VHF6|A0A649VHF6_9CAUD Antirepressor OS=Mycobacterium phage Blexus OX=2656561 GN=45 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84PADD5 pKa = 3.8PVRR8 pKa = 11.84AAIQEE13 pKa = 4.24SLDD16 pKa = 3.61AQGDD20 pKa = 3.98GWQVAHH26 pKa = 5.88YY27 pKa = 8.9VAVVGLEE34 pKa = 4.21RR35 pKa = 11.84ITGDD39 pKa = 4.31QMDD42 pKa = 4.83LGATTVITPVGQPGYY57 pKa = 9.37VTDD60 pKa = 4.24GLVNRR65 pKa = 11.84YY66 pKa = 7.55WDD68 pKa = 3.72EE69 pKa = 4.72SDD71 pKa = 5.6DD72 pKa = 3.91EE73 pKa = 4.4

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VI43|A0A649VI43_9CAUD WhiB family transcription factor OS=Mycobacterium phage Blexus OX=2656561 GN=57 PE=3 SV=1
MM1 pKa = 7.15RR2 pKa = 11.84TSANRR7 pKa = 11.84KK8 pKa = 7.4SSRR11 pKa = 11.84TVRR14 pKa = 11.84ANNAVTCNVRR24 pKa = 11.84KK25 pKa = 9.15LQARR29 pKa = 11.84NCKK32 pKa = 10.01EE33 pKa = 4.12SGLCKK38 pKa = 10.09QWTLHH43 pKa = 5.52LFARR47 pKa = 11.84EE48 pKa = 3.77HH49 pKa = 6.49VGVNEE54 pKa = 4.16NKK56 pKa = 8.72EE57 pKa = 4.1HH58 pKa = 7.22RR59 pKa = 11.84EE60 pKa = 3.76DD61 pKa = 3.47WPFGPEE67 pKa = 3.95LKK69 pKa = 9.97RR70 pKa = 11.84HH71 pKa = 5.63RR72 pKa = 11.84EE73 pKa = 3.89RR74 pKa = 11.84VGLSQRR80 pKa = 11.84EE81 pKa = 4.11ASRR84 pKa = 11.84RR85 pKa = 11.84TTPPGSDD92 pKa = 3.27KK93 pKa = 10.79PAVSAGRR100 pKa = 11.84WKK102 pKa = 10.47QLEE105 pKa = 4.39TGWQINKK112 pKa = 8.14GTLIPIGTTASTVAAAARR130 pKa = 11.84AVQWDD135 pKa = 4.04VNEE138 pKa = 4.31ALAIAGFQQSDD149 pKa = 3.92IPPPLPEE156 pKa = 4.06PAIVRR161 pKa = 11.84YY162 pKa = 10.11SDD164 pKa = 4.6DD165 pKa = 3.43EE166 pKa = 4.17LLAEE170 pKa = 4.24VRR172 pKa = 11.84RR173 pKa = 11.84RR174 pKa = 11.84LQEE177 pKa = 3.56ARR179 pKa = 11.84NVMEE183 pKa = 4.24TAQTTRR189 pKa = 11.84TPRR192 pKa = 11.84EE193 pKa = 3.61ARR195 pKa = 11.84QDD197 pKa = 3.57QEE199 pKa = 4.53GDD201 pKa = 2.91LGARR205 pKa = 11.84PGEE208 pKa = 4.29PPQPRR213 pKa = 11.84QPRR216 pKa = 11.84ASEE219 pKa = 3.86TGPAIHH225 pKa = 6.54AHH227 pKa = 4.83VARR230 pKa = 11.84SVRR233 pKa = 11.84ARR235 pKa = 11.84QRR237 pKa = 11.84RR238 pKa = 11.84KK239 pKa = 10.29DD240 pKa = 3.26

Molecular weight: 26.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

18571

30

1238

183.9

20.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.786 ± 0.487

1.147 ± 0.142

6.505 ± 0.198

5.918 ± 0.319

3.064 ± 0.19

8.772 ± 0.654

2.219 ± 0.156

4.421 ± 0.212

3.29 ± 0.2

7.404 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.116

3.365 ± 0.165

6.149 ± 0.227

3.543 ± 0.205

6.844 ± 0.412

6.047 ± 0.241

6.376 ± 0.269

7.162 ± 0.24

2.359 ± 0.155

2.385 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski