Escherichia phage P2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; Escherichia virus magyaro

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1RZ27|M1RZ27_BPP2 Phage outer membrane lipoprotein Rz1 OS=Escherichia phage P2 OX=2681594 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 9.98TFALQGDD9 pKa = 4.3TLDD12 pKa = 4.87AICVRR17 pKa = 11.84YY18 pKa = 9.91YY19 pKa = 11.07GRR21 pKa = 11.84TDD23 pKa = 3.38GVVEE27 pKa = 4.28TVLAANPGLAEE38 pKa = 4.58LGAVLPHH45 pKa = 5.85GTAVEE50 pKa = 4.18LPDD53 pKa = 3.66VQTAPVAEE61 pKa = 4.29TVNLWEE67 pKa = 4.2

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1T2Q6|M1T2Q6_BPP2 Phage tail protein OS=Escherichia phage P2 OX=2681594 PE=4 SV=1
MM1 pKa = 7.6IGVLIVVGKK10 pKa = 9.79VLAGGEE16 pKa = 4.47PITPRR21 pKa = 11.84LFIGRR26 pKa = 11.84MLLGGFVSMVAGVVLVQFPDD46 pKa = 3.41LSLPAVCGIGSMLGIAGYY64 pKa = 8.18QVIEE68 pKa = 3.82IAIQRR73 pKa = 11.84RR74 pKa = 11.84FKK76 pKa = 11.29GRR78 pKa = 11.84GKK80 pKa = 9.83PP81 pKa = 3.39

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

9700

51

815

210.9

23.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.258 ± 0.62

1.01 ± 0.132

5.423 ± 0.245

6.175 ± 0.311

3.33 ± 0.222

6.804 ± 0.31

1.732 ± 0.174

5.464 ± 0.211

5.32 ± 0.302

9.258 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.195

3.928 ± 0.268

4.825 ± 0.386

3.856 ± 0.199

6.557 ± 0.464

5.99 ± 0.253

6.577 ± 0.376

6.577 ± 0.327

1.433 ± 0.109

2.732 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski