Dietzia sp. UCD-THP

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Dietziaceae; Dietzia; unclassified Dietzia

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3489 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A022LPY7|A0A022LPY7_9ACTN Monooxygenase OS=Dietzia sp. UCD-THP OX=1292020 GN=H483_0100790 PE=4 SV=1
MM1 pKa = 7.37AVHH4 pKa = 6.85GRR6 pKa = 11.84FLKK9 pKa = 10.75AAAAASMVAMVSAACTNGGGGEE31 pKa = 4.09SDD33 pKa = 3.76EE34 pKa = 4.12QTEE37 pKa = 4.35AGTSGVGPITFAMGSNDD54 pKa = 3.55TDD56 pKa = 3.49KK57 pKa = 11.01LRR59 pKa = 11.84PVIDD63 pKa = 3.05QWNAEE68 pKa = 4.15NPDD71 pKa = 3.4QEE73 pKa = 4.4VTLRR77 pKa = 11.84EE78 pKa = 4.21LPAEE82 pKa = 3.95QDD84 pKa = 3.61GQRR87 pKa = 11.84DD88 pKa = 3.77TLTQSLQTEE97 pKa = 4.16SGEE100 pKa = 4.12YY101 pKa = 10.45DD102 pKa = 3.78VFALDD107 pKa = 3.9VTDD110 pKa = 3.61TAYY113 pKa = 10.56FAANGWLQPIEE124 pKa = 4.46GDD126 pKa = 3.9TEE128 pKa = 4.43IDD130 pKa = 3.14TSGLIEE136 pKa = 4.33AAVDD140 pKa = 3.4SATYY144 pKa = 10.68NGTLYY149 pKa = 10.96GIPQNTNAQLLYY161 pKa = 10.73YY162 pKa = 9.59RR163 pKa = 11.84TDD165 pKa = 3.74LQPEE169 pKa = 4.28APADD173 pKa = 3.52WDD175 pKa = 3.85ALVASCEE182 pKa = 4.05AAEE185 pKa = 4.65GADD188 pKa = 3.71VDD190 pKa = 4.64CLQTQLSLYY199 pKa = 10.29EE200 pKa = 4.32GLTVAATQFIHH211 pKa = 5.87SWGGRR216 pKa = 11.84VVGDD220 pKa = 4.2DD221 pKa = 3.65GQTPEE226 pKa = 5.55LDD228 pKa = 3.26TDD230 pKa = 3.49QARR233 pKa = 11.84AGLTALVDD241 pKa = 4.42AYY243 pKa = 11.01QNGVIPPQTDD253 pKa = 2.65AFTEE257 pKa = 4.26EE258 pKa = 3.88EE259 pKa = 4.28TAQGFLAGEE268 pKa = 4.18TMYY271 pKa = 10.87SYY273 pKa = 10.98NWPYY277 pKa = 10.06MYY279 pKa = 10.45EE280 pKa = 4.18SGQSDD285 pKa = 3.76PTSQVQGRR293 pKa = 11.84FEE295 pKa = 3.87VAPIVGQDD303 pKa = 3.17GAGRR307 pKa = 11.84STLGGYY313 pKa = 10.17NNAINVFSEE322 pKa = 4.26NKK324 pKa = 8.83ATAQAFLEE332 pKa = 4.47YY333 pKa = 9.91IISEE337 pKa = 4.17DD338 pKa = 3.48VQMSFAEE345 pKa = 4.23QSFPPVLSSIYY356 pKa = 10.34DD357 pKa = 3.68DD358 pKa = 4.62EE359 pKa = 6.06ALQQQFPYY367 pKa = 10.12MPALKK372 pKa = 10.22AALEE376 pKa = 4.16NAEE379 pKa = 4.36PRR381 pKa = 11.84PVTPYY386 pKa = 10.34YY387 pKa = 9.83PAVSKK392 pKa = 10.85AIQDD396 pKa = 3.48NTFAALRR403 pKa = 11.84GQKK406 pKa = 9.8NVEE409 pKa = 3.85QALTDD414 pKa = 3.36MSAAIDD420 pKa = 3.54QAAQQ424 pKa = 3.04

Molecular weight: 45.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A022LUP8|A0A022LUP8_9ACTN Acyl dehydratase OS=Dietzia sp. UCD-THP OX=1292020 GN=H483_0100935 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 9.45VHH19 pKa = 5.31GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84GIVGARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.68GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3489

0

3489

1118474

32

3134

320.6

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.843 ± 0.056

0.753 ± 0.011

6.614 ± 0.034

5.865 ± 0.035

2.757 ± 0.022

9.551 ± 0.045

2.196 ± 0.021

3.864 ± 0.029

1.733 ± 0.027

9.708 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.015

1.728 ± 0.02

5.771 ± 0.034

2.562 ± 0.022

7.767 ± 0.047

5.623 ± 0.023

6.179 ± 0.027

9.125 ± 0.044

1.437 ± 0.017

1.907 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski