Sanxia tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGD9|A0A1L3KGD9_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 1 OX=1923385 PE=4 SV=1
MM1 pKa = 7.75DD2 pKa = 3.54MWRR5 pKa = 11.84ILRR8 pKa = 11.84RR9 pKa = 11.84LCFVRR14 pKa = 11.84RR15 pKa = 11.84NRR17 pKa = 11.84CILQMATKK25 pKa = 9.86WLGNLNLLCSRR36 pKa = 11.84ISIVDD41 pKa = 3.91VISLGRR47 pKa = 11.84KK48 pKa = 7.33YY49 pKa = 9.86LTYY52 pKa = 10.81GVLVQGKK59 pKa = 8.89VGQPQLVTFLSTRR72 pKa = 11.84LFIRR76 pKa = 11.84PFQTNIVLHH85 pKa = 5.45TCRR88 pKa = 11.84KK89 pKa = 9.19LLLSSRR95 pKa = 11.84LGLQCYY101 pKa = 9.12PKK103 pKa = 10.68GFNLKK108 pKa = 9.84FVAQNPEE115 pKa = 3.69HH116 pKa = 5.98ATVFGKK122 pKa = 10.66LLEE125 pKa = 4.2QLPSSNWFLNNILARR140 pKa = 11.84FKK142 pKa = 11.1LGINNPVLAICSKK155 pKa = 9.48VSPSIGLSGPEE166 pKa = 3.56KK167 pKa = 10.89VIIKK171 pKa = 8.16MAKK174 pKa = 8.33TKK176 pKa = 10.51NKK178 pKa = 10.32NMTQKK183 pKa = 10.68VGATAMGYY191 pKa = 9.52SNSYY195 pKa = 8.05KK196 pKa = 8.67TPKK199 pKa = 8.38MQTVGSSIIVKK210 pKa = 9.82HH211 pKa = 6.25KK212 pKa = 10.18EE213 pKa = 3.85LIRR216 pKa = 11.84QVASQSAFNVAEE228 pKa = 3.86ARR230 pKa = 11.84INPADD235 pKa = 4.04LEE237 pKa = 4.51SFPWLAGIATRR248 pKa = 11.84YY249 pKa = 7.85EE250 pKa = 4.09KK251 pKa = 11.14YY252 pKa = 10.13RR253 pKa = 11.84FLKK256 pKa = 10.21MKK258 pKa = 8.96FTLVPQVPTTAPGSLGLYY276 pKa = 9.95FDD278 pKa = 5.13YY279 pKa = 11.21DD280 pKa = 3.88ATDD283 pKa = 3.84LPAPNATQFFSNLNAVTTQIWMEE306 pKa = 3.98ASTVVLPQPMEE317 pKa = 4.2LYY319 pKa = 10.71CATKK323 pKa = 10.75YY324 pKa = 10.69DD325 pKa = 3.76GQNPKK330 pKa = 9.32WFDD333 pKa = 3.38YY334 pKa = 11.2GRR336 pKa = 11.84IKK338 pKa = 11.09YY339 pKa = 9.47FMQSSVQCLGYY350 pKa = 10.79LFVEE354 pKa = 4.71YY355 pKa = 9.95EE356 pKa = 4.24VEE358 pKa = 3.89LSKK361 pKa = 10.44PAQTVGQISRR371 pKa = 11.84FSGHH375 pKa = 7.17AYY377 pKa = 8.05WQTLSQSADD386 pKa = 3.48VPVGTPTWIEE396 pKa = 3.79AVTVDD401 pKa = 4.47GAPGQISVPYY411 pKa = 8.74SGWYY415 pKa = 8.85RR416 pKa = 11.84VQAICNSTVNTATLIEE432 pKa = 4.27ICGSAIAALPLGLDD446 pKa = 3.34ANTNPKK452 pKa = 8.99TYY454 pKa = 9.91AYY456 pKa = 8.44TAIVFLTANVPFYY469 pKa = 10.95AFKK472 pKa = 10.94VSAGFVDD479 pKa = 4.37VTADD483 pKa = 3.57PNGADD488 pKa = 3.34SPLGIEE494 pKa = 4.59LEE496 pKa = 4.41LVGPVV501 pKa = 3.02

Molecular weight: 55.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGZ3|A0A1L3KGZ3_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 1 OX=1923385 PE=4 SV=1
MM1 pKa = 7.36CRR3 pKa = 11.84CKK5 pKa = 10.47KK6 pKa = 10.34RR7 pKa = 11.84LLLRR11 pKa = 11.84KK12 pKa = 8.98MNGSSKK18 pKa = 10.57SRR20 pKa = 11.84VVHH23 pKa = 5.35ATLLARR29 pKa = 11.84QLLQKK34 pKa = 10.4QQVTLINITTGRR46 pKa = 11.84VLVILRR52 pKa = 11.84LTGWFLGTLFARR64 pKa = 11.84LSKK67 pKa = 10.47SSKK70 pKa = 9.51FRR72 pKa = 11.84SGALTCVVLWIRR84 pKa = 11.84PSMRR88 pKa = 11.84SCTFHH93 pKa = 8.16GIICIALRR101 pKa = 11.84LGVVVNKK108 pKa = 9.91WKK110 pKa = 10.47DD111 pKa = 3.08SRR113 pKa = 11.84LRR115 pKa = 11.84SRR117 pKa = 11.84LDD119 pKa = 3.13SQIFRR124 pKa = 11.84DD125 pKa = 4.22SHH127 pKa = 6.75SNWPNQLAVSPYY139 pKa = 10.49AVGWDD144 pKa = 3.26SWLAACC150 pKa = 6.05

Molecular weight: 17.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1178

150

527

392.7

44.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 1.009

2.377 ± 0.48

3.48 ± 0.504

3.565 ± 1.162

4.669 ± 0.394

6.367 ± 0.313

1.868 ± 0.571

4.924 ± 0.52

5.603 ± 0.119

11.036 ± 1.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.287

4.244 ± 0.511

4.669 ± 0.921

6.112 ± 1.006

6.452 ± 1.572

7.555 ± 0.912

5.348 ± 0.89

7.64 ± 0.343

2.037 ± 0.576

3.396 ± 0.823
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski