Influenza B virus (strain B/Singapore/222/1979)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Betainfluenzavirus; Influenza B virus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P12600|NS1_INBSI Non-structural protein 1 OS=Influenza B virus (strain B/Singapore/222/1979) OX=107417 GN=NS PE=3 SV=1
MM1 pKa = 7.72DD2 pKa = 4.03TFITRR7 pKa = 11.84NFQTTIIQKK16 pKa = 10.29AKK18 pKa = 8.58NTMAEE23 pKa = 4.07FMEE26 pKa = 5.03DD27 pKa = 3.9PEE29 pKa = 4.82LQPAMLFNICVHH41 pKa = 6.68LEE43 pKa = 3.78VCYY46 pKa = 10.78VISDD50 pKa = 3.68MNFLDD55 pKa = 4.43EE56 pKa = 4.43EE57 pKa = 4.77GKK59 pKa = 10.67AYY61 pKa = 8.15TALEE65 pKa = 4.33GQGKK69 pKa = 7.68EE70 pKa = 3.94QNLRR74 pKa = 11.84PQYY77 pKa = 10.54EE78 pKa = 4.57VIEE81 pKa = 4.19GMPRR85 pKa = 11.84NIAWMVQRR93 pKa = 11.84SLAQEE98 pKa = 4.19HH99 pKa = 6.59GIEE102 pKa = 4.28TPKK105 pKa = 10.97YY106 pKa = 9.38LADD109 pKa = 3.81LFDD112 pKa = 4.22YY113 pKa = 8.3KK114 pKa = 9.99TKK116 pKa = 10.82RR117 pKa = 11.84FIEE120 pKa = 4.0VGITKK125 pKa = 10.32GLADD129 pKa = 4.42DD130 pKa = 4.63YY131 pKa = 11.19FWKK134 pKa = 10.59KK135 pKa = 10.28KK136 pKa = 9.87EE137 pKa = 3.82KK138 pKa = 10.49LGNSMEE144 pKa = 4.41LMIFSYY150 pKa = 10.74NQDD153 pKa = 2.93YY154 pKa = 11.2SLSNEE159 pKa = 3.9SSLDD163 pKa = 3.56EE164 pKa = 4.4EE165 pKa = 4.86GKK167 pKa = 10.97GRR169 pKa = 11.84VLSRR173 pKa = 11.84LTEE176 pKa = 4.09LQAEE180 pKa = 4.6LSLKK184 pKa = 10.43NLWQVLIGEE193 pKa = 4.28EE194 pKa = 4.54DD195 pKa = 3.28IEE197 pKa = 4.69KK198 pKa = 11.15GIDD201 pKa = 3.45FKK203 pKa = 11.36LGQTISRR210 pKa = 11.84LRR212 pKa = 11.84DD213 pKa = 3.1ISVPAGFSNFEE224 pKa = 4.0GMRR227 pKa = 11.84SYY229 pKa = 11.04IDD231 pKa = 4.25NIDD234 pKa = 3.38PKK236 pKa = 11.14GAIEE240 pKa = 4.13RR241 pKa = 11.84NLARR245 pKa = 11.84MSPLVSVTPKK255 pKa = 9.85KK256 pKa = 10.83LKK258 pKa = 10.23WEE260 pKa = 4.3DD261 pKa = 3.41LRR263 pKa = 11.84PIGPHH268 pKa = 6.43IYY270 pKa = 9.79NHH272 pKa = 6.28EE273 pKa = 4.17LPEE276 pKa = 4.04VPYY279 pKa = 10.96NAFLLMSDD287 pKa = 3.99EE288 pKa = 4.95LGLANMTEE296 pKa = 4.57GKK298 pKa = 9.69SKK300 pKa = 10.66KK301 pKa = 9.72PKK303 pKa = 9.48TLAKK307 pKa = 10.0EE308 pKa = 4.07CLEE311 pKa = 4.39KK312 pKa = 11.23YY313 pKa = 8.63STLRR317 pKa = 11.84DD318 pKa = 3.36QTDD321 pKa = 4.33PILIMKK327 pKa = 9.41SEE329 pKa = 4.03KK330 pKa = 10.78ANEE333 pKa = 3.8NFLWKK338 pKa = 10.42LWRR341 pKa = 11.84DD342 pKa = 3.76CVNTISNEE350 pKa = 3.98EE351 pKa = 4.1TSNEE355 pKa = 3.91LQKK358 pKa = 10.16TNYY361 pKa = 9.82AKK363 pKa = 10.3WATGDD368 pKa = 3.24GLTYY372 pKa = 10.2QKK374 pKa = 10.63IMKK377 pKa = 9.33EE378 pKa = 3.96VAIDD382 pKa = 3.9DD383 pKa = 3.68EE384 pKa = 5.15TMCQEE389 pKa = 4.25EE390 pKa = 4.1PKK392 pKa = 10.31IPNKK396 pKa = 10.07CRR398 pKa = 11.84VAAWVQTEE406 pKa = 4.14MNLLSTLTSKK416 pKa = 10.85RR417 pKa = 11.84ALDD420 pKa = 3.91LPEE423 pKa = 5.27IGPDD427 pKa = 3.43VAPVEE432 pKa = 4.56HH433 pKa = 6.53VGSEE437 pKa = 3.58RR438 pKa = 11.84RR439 pKa = 11.84KK440 pKa = 9.81YY441 pKa = 9.86FVNEE445 pKa = 3.34INYY448 pKa = 9.25CKK450 pKa = 10.56ASTVMMKK457 pKa = 10.46YY458 pKa = 10.74VLFHH462 pKa = 6.42TSLLNEE468 pKa = 4.68SNASMGKK475 pKa = 9.99YY476 pKa = 9.62KK477 pKa = 10.59VIPITNRR484 pKa = 11.84VNEE487 pKa = 4.2KK488 pKa = 10.96GEE490 pKa = 4.3SFDD493 pKa = 3.5MLYY496 pKa = 11.24GLAVKK501 pKa = 9.91GQSHH505 pKa = 6.71LRR507 pKa = 11.84GDD509 pKa = 3.87TDD511 pKa = 3.58VVTVVTFEE519 pKa = 4.42FSSTDD524 pKa = 3.3PRR526 pKa = 11.84VDD528 pKa = 3.05SGKK531 pKa = 8.47WPKK534 pKa = 9.4YY535 pKa = 6.84TVFRR539 pKa = 11.84IGSLFVSGRR548 pKa = 11.84EE549 pKa = 3.75KK550 pKa = 10.61SVYY553 pKa = 10.09LYY555 pKa = 11.01CRR557 pKa = 11.84VNGTNKK563 pKa = 9.61IQMKK567 pKa = 8.84WGMEE571 pKa = 3.68ARR573 pKa = 11.84RR574 pKa = 11.84CLLQSMQQMEE584 pKa = 5.02AIVEE588 pKa = 4.22QEE590 pKa = 4.1SSIQGYY596 pKa = 11.18DD597 pKa = 3.05MTKK600 pKa = 10.49ACFKK604 pKa = 9.93EE605 pKa = 4.19DD606 pKa = 3.17RR607 pKa = 11.84VNSPKK612 pKa = 10.09TFSIGTQEE620 pKa = 3.93GKK622 pKa = 10.13LVKK625 pKa = 10.6GSFGKK630 pKa = 10.38ALRR633 pKa = 11.84VIFTKK638 pKa = 10.82CLMHH642 pKa = 6.24YY643 pKa = 10.55VFGNAQLEE651 pKa = 4.61GFSAEE656 pKa = 4.01SRR658 pKa = 11.84RR659 pKa = 11.84LLLLIQALKK668 pKa = 10.37DD669 pKa = 3.68RR670 pKa = 11.84KK671 pKa = 10.3GPWVFDD677 pKa = 3.85LEE679 pKa = 4.38GMYY682 pKa = 10.41SGIEE686 pKa = 3.85EE687 pKa = 4.9CISNNPWVIQSAYY700 pKa = 8.73WFNEE704 pKa = 3.19WLGFEE709 pKa = 4.34KK710 pKa = 10.6EE711 pKa = 4.12GSKK714 pKa = 10.62VLEE717 pKa = 4.4SVDD720 pKa = 4.31EE721 pKa = 4.55IMDD724 pKa = 3.72EE725 pKa = 4.12

Molecular weight:
83.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9QLL3|Q9QLL3_INBSI Polymerase basic protein 2 OS=Influenza B virus (strain B/Singapore/222/1979) OX=107417 GN=PB2 PE=3 SV=1
MM1 pKa = 7.2TLAKK5 pKa = 10.05IEE7 pKa = 4.27LLKK10 pKa = 10.82QLLRR14 pKa = 11.84DD15 pKa = 3.91NEE17 pKa = 4.23AKK19 pKa = 9.7TVLKK23 pKa = 9.15QTTVDD28 pKa = 3.07QYY30 pKa = 11.86NIIRR34 pKa = 11.84KK35 pKa = 8.85FNTSRR40 pKa = 11.84IEE42 pKa = 4.0KK43 pKa = 10.24NPSLRR48 pKa = 11.84MKK50 pKa = 9.92WAMCSNFPLALTKK63 pKa = 10.87GDD65 pKa = 3.51MANRR69 pKa = 11.84IPLEE73 pKa = 3.92YY74 pKa = 10.33KK75 pKa = 10.63GIQLKK80 pKa = 9.38TNAEE84 pKa = 4.42DD85 pKa = 3.46IGTKK89 pKa = 10.43GQMCSIAAVTWWNTYY104 pKa = 10.1GPIGDD109 pKa = 3.74TEE111 pKa = 4.35GFEE114 pKa = 4.22KK115 pKa = 10.73VYY117 pKa = 11.02EE118 pKa = 4.3SFFLRR123 pKa = 11.84KK124 pKa = 8.78MRR126 pKa = 11.84LDD128 pKa = 3.16NATWGRR134 pKa = 11.84ITFGPVEE141 pKa = 4.17RR142 pKa = 11.84VRR144 pKa = 11.84KK145 pKa = 9.27RR146 pKa = 11.84VLLNPLTKK154 pKa = 10.41EE155 pKa = 3.99MPPDD159 pKa = 3.45EE160 pKa = 4.69ASNVIMEE167 pKa = 4.24ILFPKK172 pKa = 9.72EE173 pKa = 3.27AGIPRR178 pKa = 11.84EE179 pKa = 4.2STWIHH184 pKa = 6.43RR185 pKa = 11.84EE186 pKa = 3.8LIKK189 pKa = 10.54EE190 pKa = 3.96KK191 pKa = 10.54RR192 pKa = 11.84EE193 pKa = 3.94KK194 pKa = 11.07LKK196 pKa = 10.21GTMITPIVLAYY207 pKa = 8.9MLEE210 pKa = 4.26RR211 pKa = 11.84EE212 pKa = 4.28LVARR216 pKa = 11.84RR217 pKa = 11.84RR218 pKa = 11.84FLPVAGATSAEE229 pKa = 4.51FIEE232 pKa = 4.74MLHH235 pKa = 6.25CLQGEE240 pKa = 4.23NWRR243 pKa = 11.84QIYY246 pKa = 10.2HH247 pKa = 6.78PGGNKK252 pKa = 8.44LTEE255 pKa = 4.17SRR257 pKa = 11.84SQSMIVACRR266 pKa = 11.84KK267 pKa = 9.17IIRR270 pKa = 11.84RR271 pKa = 11.84SIVASNPLEE280 pKa = 4.1LAVEE284 pKa = 4.58IANKK288 pKa = 8.86TVIDD292 pKa = 3.95TEE294 pKa = 4.23PLKK297 pKa = 11.19SCLAAIDD304 pKa = 4.49GGDD307 pKa = 3.66VACDD311 pKa = 3.12IMRR314 pKa = 11.84AALGLKK320 pKa = 9.13IRR322 pKa = 11.84QRR324 pKa = 11.84QRR326 pKa = 11.84FGRR329 pKa = 11.84LEE331 pKa = 4.07LKK333 pKa = 10.28RR334 pKa = 11.84ISGRR338 pKa = 11.84GFKK341 pKa = 10.29NDD343 pKa = 3.3EE344 pKa = 4.59EE345 pKa = 4.2ILIGNGTIQKK355 pKa = 10.0IGIWDD360 pKa = 3.73GEE362 pKa = 4.47EE363 pKa = 3.99EE364 pKa = 4.11FHH366 pKa = 6.94VRR368 pKa = 11.84CGEE371 pKa = 3.88CRR373 pKa = 11.84GILKK377 pKa = 9.91KK378 pKa = 10.83SKK380 pKa = 9.48MRR382 pKa = 11.84MEE384 pKa = 4.32KK385 pKa = 10.82LLINSAKK392 pKa = 10.33KK393 pKa = 10.58EE394 pKa = 3.99DD395 pKa = 3.88MKK397 pKa = 11.48DD398 pKa = 3.71LIILCMVFSQDD409 pKa = 2.31TRR411 pKa = 11.84MFQGVRR417 pKa = 11.84GEE419 pKa = 4.12INFLNRR425 pKa = 11.84AGQLLSPMYY434 pKa = 10.01QLQRR438 pKa = 11.84YY439 pKa = 7.37FLNRR443 pKa = 11.84SNDD446 pKa = 3.9LFDD449 pKa = 2.96QWGYY453 pKa = 10.98EE454 pKa = 4.08EE455 pKa = 4.74SPKK458 pKa = 10.73ASEE461 pKa = 3.57LHH463 pKa = 6.88GINEE467 pKa = 4.33LMNASDD473 pKa = 3.92YY474 pKa = 8.88TLKK477 pKa = 11.05GVVVTKK483 pKa = 10.76NVIDD487 pKa = 4.64DD488 pKa = 4.65FSSTEE493 pKa = 4.04TEE495 pKa = 4.03KK496 pKa = 11.31VSITKK501 pKa = 10.01NLSLIKK507 pKa = 9.88RR508 pKa = 11.84TGEE511 pKa = 4.12VIMGANDD518 pKa = 3.09ISEE521 pKa = 5.01LEE523 pKa = 4.23SQAQLMITYY532 pKa = 7.68DD533 pKa = 3.87TPKK536 pKa = 8.17MWEE539 pKa = 3.77MGTTKK544 pKa = 10.43EE545 pKa = 4.29LVQNTYY551 pKa = 8.21QWVLKK556 pKa = 10.91NLVTLKK562 pKa = 10.93AQFLLGKK569 pKa = 9.96EE570 pKa = 5.88DD571 pKa = 3.54MFQWDD576 pKa = 3.33AFEE579 pKa = 4.66AFEE582 pKa = 5.18SIIPQKK588 pKa = 10.32MAGQYY593 pKa = 10.54SGFARR598 pKa = 11.84AVLKK602 pKa = 9.91QMRR605 pKa = 11.84DD606 pKa = 3.47QEE608 pKa = 4.51VMKK611 pKa = 10.1TDD613 pKa = 3.47QFIKK617 pKa = 10.67LLPFCFSPPKK627 pKa = 10.06LRR629 pKa = 11.84SNGEE633 pKa = 3.67PYY635 pKa = 10.49QFLRR639 pKa = 11.84LILKK643 pKa = 10.43GGGEE647 pKa = 4.11NFIEE651 pKa = 4.26VRR653 pKa = 11.84KK654 pKa = 10.13GSPLFSYY661 pKa = 10.68NPQTEE666 pKa = 4.12VLTICGRR673 pKa = 11.84MMSLKK678 pKa = 10.66GKK680 pKa = 10.2IEE682 pKa = 4.01DD683 pKa = 3.72EE684 pKa = 3.95EE685 pKa = 5.12RR686 pKa = 11.84NRR688 pKa = 11.84SMGNAVLAGFLVSGKK703 pKa = 10.1YY704 pKa = 10.53DD705 pKa = 3.9PDD707 pKa = 5.06LGDD710 pKa = 3.6FKK712 pKa = 10.87TIEE715 pKa = 4.29EE716 pKa = 4.4LEE718 pKa = 4.1KK719 pKa = 11.0LKK721 pKa = 10.75PGEE724 pKa = 3.97KK725 pKa = 10.57ANILLYY731 pKa = 10.07QGKK734 pKa = 7.97PVKK737 pKa = 9.66VVKK740 pKa = 10.37RR741 pKa = 11.84KK742 pKa = 9.92RR743 pKa = 11.84YY744 pKa = 8.87SALSNDD750 pKa = 2.67ISQGIKK756 pKa = 8.29RR757 pKa = 11.84QRR759 pKa = 11.84MTVEE763 pKa = 3.77SMGWALSS770 pKa = 3.61

Molecular weight:
87.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4034

100

770

448.2

50.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.049 ± 0.429

2.256 ± 0.339

4.611 ± 0.268

7.313 ± 0.742

3.718 ± 0.296

7.362 ± 0.439

1.71 ± 0.309

6.619 ± 0.419

7.536 ± 0.368

9.147 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.743 ± 0.407

5.429 ± 0.593

4.09 ± 0.359

3.074 ± 0.396

5.181 ± 0.408

6.544 ± 0.392

6.197 ± 0.53

5.231 ± 0.329

1.264 ± 0.219

2.925 ± 0.311

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski