Amino acid dipepetide frequency for Enterocytozoon hepatopenaei

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.6AlaAla: 1.6 ± 0.065
0.7AlaCys: 0.7 ± 0.03
1.506AlaAsp: 1.506 ± 0.044
2.206AlaGlu: 2.206 ± 0.07
2.197AlaPhe: 2.197 ± 0.059
1.333AlaGly: 1.333 ± 0.057
0.588AlaHis: 0.588 ± 0.027
3.099AlaIle: 3.099 ± 0.068
3.11AlaLys: 3.11 ± 0.073
3.418AlaLeu: 3.418 ± 0.085
0.84AlaMet: 0.84 ± 0.034
2.244AlaAsn: 2.244 ± 0.061
0.825AlaPro: 0.825 ± 0.037
1.017AlaGln: 1.017 ± 0.042
1.041AlaArg: 1.041 ± 0.044
2.045AlaSer: 2.045 ± 0.062
1.703AlaThr: 1.703 ± 0.059
2.081AlaVal: 2.081 ± 0.065
0.209AlaTrp: 0.209 ± 0.019
1.583AlaTyr: 1.583 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.751CysAla: 0.751 ± 0.032
0.421CysCys: 0.421 ± 0.027
1.063CysAsp: 1.063 ± 0.036
1.2CysGlu: 1.2 ± 0.045
1.21CysPhe: 1.21 ± 0.04
0.908CysGly: 0.908 ± 0.039
0.248CysHis: 0.248 ± 0.019
1.751CysIle: 1.751 ± 0.049
2.023CysLys: 2.023 ± 0.054
1.79CysLeu: 1.79 ± 0.057
0.494CysMet: 0.494 ± 0.024
1.877CysAsn: 1.877 ± 0.08
0.397CysPro: 0.397 ± 0.025
0.378CysGln: 0.378 ± 0.024
0.553CysArg: 0.553 ± 0.027
1.272CysSer: 1.272 ± 0.037
0.874CysThr: 0.874 ± 0.038
1.107CysVal: 1.107 ± 0.04
0.088CysTrp: 0.088 ± 0.012
0.832CysTyr: 0.832 ± 0.035
0.0CysXaa: 0.0 ± 0.0
Asp
1.921AspAla: 1.921 ± 0.052
0.866AspCys: 0.866 ± 0.036
2.821AspAsp: 2.821 ± 0.069
3.869AspGlu: 3.869 ± 0.069
3.791AspPhe: 3.791 ± 0.085
1.921AspGly: 1.921 ± 0.07
0.672AspHis: 0.672 ± 0.031
5.414AspIle: 5.414 ± 0.112
5.442AspLys: 5.442 ± 0.102
4.809AspLeu: 4.809 ± 0.081
1.192AspMet: 1.192 ± 0.044
4.823AspAsn: 4.823 ± 0.157
1.007AspPro: 1.007 ± 0.038
1.065AspGln: 1.065 ± 0.04
1.337AspArg: 1.337 ± 0.051
3.276AspSer: 3.276 ± 0.061
2.352AspThr: 2.352 ± 0.05
3.013AspVal: 3.013 ± 0.065
0.256AspTrp: 0.256 ± 0.021
2.074AspTyr: 2.074 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
2.248GluAla: 2.248 ± 0.073
1.312GluCys: 1.312 ± 0.045
3.81GluAsp: 3.81 ± 0.078
7.692GluGlu: 7.692 ± 0.163
3.577GluPhe: 3.577 ± 0.066
2.397GluGly: 2.397 ± 0.063
1.207GluHis: 1.207 ± 0.039
7.772GluIle: 7.772 ± 0.142
9.388GluLys: 9.388 ± 0.168
5.508GluLeu: 5.508 ± 0.095
2.136GluMet: 2.136 ± 0.052
7.87GluAsn: 7.87 ± 0.12
1.057GluPro: 1.057 ± 0.036
2.124GluGln: 2.124 ± 0.061
2.255GluArg: 2.255 ± 0.055
4.008GluSer: 4.008 ± 0.088
3.632GluThr: 3.632 ± 0.08
3.283GluVal: 3.283 ± 0.08
0.394GluTrp: 0.394 ± 0.022
3.434GluTyr: 3.434 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
1.842PheAla: 1.842 ± 0.062
1.331PheCys: 1.331 ± 0.045
3.32PheAsp: 3.32 ± 0.071
3.961PheGlu: 3.961 ± 0.088
3.527PhePhe: 3.527 ± 0.097
2.051PheGly: 2.051 ± 0.069
0.678PheHis: 0.678 ± 0.034
5.787PheIle: 5.787 ± 0.089
5.753PheLys: 5.753 ± 0.097
6.668PheLeu: 6.668 ± 0.112
1.555PheMet: 1.555 ± 0.048
4.047PheAsn: 4.047 ± 0.08
0.96PhePro: 0.96 ± 0.043
1.047PheGln: 1.047 ± 0.039
1.334PheArg: 1.334 ± 0.046
3.995PheSer: 3.995 ± 0.079
3.087PheThr: 3.087 ± 0.065
3.566PheVal: 3.566 ± 0.068
0.305PheTrp: 0.305 ± 0.02
3.651PheTyr: 3.651 ± 0.076
0.0PheXaa: 0.0 ± 0.0
Gly
1.359GlyAla: 1.359 ± 0.043
0.719GlyCys: 0.719 ± 0.034
1.675GlyAsp: 1.675 ± 0.059
2.41GlyGlu: 2.41 ± 0.061
2.082GlyPhe: 2.082 ± 0.054
1.605GlyGly: 1.605 ± 0.064
0.651GlyHis: 0.651 ± 0.034
3.447GlyIle: 3.447 ± 0.082
4.088GlyLys: 4.088 ± 0.09
2.688GlyLeu: 2.688 ± 0.065
0.989GlyMet: 0.989 ± 0.039
2.685GlyAsn: 2.685 ± 0.076
0.639GlyPro: 0.639 ± 0.034
0.877GlyGln: 0.877 ± 0.031
1.146GlyArg: 1.146 ± 0.044
2.259GlySer: 2.259 ± 0.067
1.805GlyThr: 1.805 ± 0.064
2.096GlyVal: 2.096 ± 0.062
0.218GlyTrp: 0.218 ± 0.015
1.579GlyTyr: 1.579 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
0.645HisAla: 0.645 ± 0.031
0.31HisCys: 0.31 ± 0.02
0.762HisAsp: 0.762 ± 0.037
1.114HisGlu: 1.114 ± 0.042
1.106HisPhe: 1.106 ± 0.039
0.678HisGly: 0.678 ± 0.03
0.305HisHis: 0.305 ± 0.019
1.513HisIle: 1.513 ± 0.043
2.053HisLys: 2.053 ± 0.059
1.521HisLeu: 1.521 ± 0.047
0.441HisMet: 0.441 ± 0.026
1.737HisAsn: 1.737 ± 0.072
0.464HisPro: 0.464 ± 0.031
0.422HisGln: 0.422 ± 0.025
0.522HisArg: 0.522 ± 0.029
1.2HisSer: 1.2 ± 0.041
0.87HisThr: 0.87 ± 0.036
1.034HisVal: 1.034 ± 0.044
0.107HisTrp: 0.107 ± 0.011
0.672HisTyr: 0.672 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
2.859IleAla: 2.859 ± 0.063
2.239IleCys: 2.239 ± 0.071
4.902IleAsp: 4.902 ± 0.102
6.441IleGlu: 6.441 ± 0.107
5.883IlePhe: 5.883 ± 0.1
2.873IleGly: 2.873 ± 0.071
1.672IleHis: 1.672 ± 0.057
7.425IleIle: 7.425 ± 0.153
10.609IleLys: 10.609 ± 0.12
10.265IleLeu: 10.265 ± 0.196
1.721IleMet: 1.721 ± 0.049
8.862IleAsn: 8.862 ± 0.182
1.957IlePro: 1.957 ± 0.054
2.492IleGln: 2.492 ± 0.066
2.438IleArg: 2.438 ± 0.067
6.575IleSer: 6.575 ± 0.107
4.336IleThr: 4.336 ± 0.098
4.374IleVal: 4.374 ± 0.092
0.406IleTrp: 0.406 ± 0.025
4.773IleTyr: 4.773 ± 0.123
0.0IleXaa: 0.0 ± 0.0
Lys
3.186LysAla: 3.186 ± 0.07
1.873LysCys: 1.873 ± 0.058
5.292LysAsp: 5.292 ± 0.1
10.667LysGlu: 10.667 ± 0.173
4.228LysPhe: 4.228 ± 0.082
3.264LysGly: 3.264 ± 0.074
2.457LysHis: 2.457 ± 0.058
10.819LysIle: 10.819 ± 0.129
12.446LysLys: 12.446 ± 0.167
8.327LysLeu: 8.327 ± 0.108
2.97LysMet: 2.97 ± 0.064
11.413LysAsn: 11.413 ± 0.163
2.069LysPro: 2.069 ± 0.055
3.519LysGln: 3.519 ± 0.087
3.706LysArg: 3.706 ± 0.072
5.807LysSer: 5.807 ± 0.103
6.258LysThr: 6.258 ± 0.108
4.982LysVal: 4.982 ± 0.083
0.521LysTrp: 0.521 ± 0.029
5.834LysTyr: 5.834 ± 0.125
0.0LysXaa: 0.0 ± 0.0
Leu
2.691LeuAla: 2.691 ± 0.082
2.121LeuCys: 2.121 ± 0.057
4.296LeuAsp: 4.296 ± 0.082
6.421LeuGlu: 6.421 ± 0.124
5.932LeuPhe: 5.932 ± 0.11
3.043LeuGly: 3.043 ± 0.067
1.871LeuHis: 1.871 ± 0.058
6.774LeuIle: 6.774 ± 0.123
9.999LeuLys: 9.999 ± 0.147
8.749LeuLeu: 8.749 ± 0.13
1.896LeuMet: 1.896 ± 0.056
8.104LeuAsn: 8.104 ± 0.115
2.188LeuPro: 2.188 ± 0.054
2.72LeuGln: 2.72 ± 0.061
2.799LeuArg: 2.799 ± 0.06
6.327LeuSer: 6.327 ± 0.098
4.511LeuThr: 4.511 ± 0.119
4.429LeuVal: 4.429 ± 0.075
0.457LeuTrp: 0.457 ± 0.028
4.772LeuTyr: 4.772 ± 0.125
0.0LeuXaa: 0.0 ± 0.0
Met
0.945MetAla: 0.945 ± 0.033
0.487MetCys: 0.487 ± 0.025
1.333MetAsp: 1.333 ± 0.036
1.711MetGlu: 1.711 ± 0.048
1.718MetPhe: 1.718 ± 0.052
0.859MetGly: 0.859 ± 0.04
0.602MetHis: 0.602 ± 0.025
1.787MetIle: 1.787 ± 0.051
1.937MetLys: 1.937 ± 0.051
2.482MetLeu: 2.482 ± 0.054
0.428MetMet: 0.428 ± 0.025
1.423MetAsn: 1.423 ± 0.036
0.591MetPro: 0.591 ± 0.028
0.755MetGln: 0.755 ± 0.032
0.649MetArg: 0.649 ± 0.034
1.578MetSer: 1.578 ± 0.042
1.005MetThr: 1.005 ± 0.037
1.462MetVal: 1.462 ± 0.05
0.121MetTrp: 0.121 ± 0.013
1.075MetTyr: 1.075 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
2.838AsnAla: 2.838 ± 0.065
1.38AsnCys: 1.38 ± 0.047
5.192AsnAsp: 5.192 ± 0.125
5.74AsnGlu: 5.74 ± 0.115
4.378AsnPhe: 4.378 ± 0.089
2.793AsnGly: 2.793 ± 0.073
1.204AsnHis: 1.204 ± 0.041
11.998AsnIle: 11.998 ± 0.337
10.705AsnLys: 10.705 ± 0.151
7.597AsnLeu: 7.597 ± 0.126
1.833AsnMet: 1.833 ± 0.05
13.183AsnAsn: 13.183 ± 0.459
1.548AsnPro: 1.548 ± 0.055
2.028AsnGln: 2.028 ± 0.062
2.065AsnArg: 2.065 ± 0.063
6.758AsnSer: 6.758 ± 0.151
7.946AsnThr: 7.946 ± 0.266
4.425AsnVal: 4.425 ± 0.081
0.344AsnTrp: 0.344 ± 0.021
3.439AsnTyr: 3.439 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
0.789ProAla: 0.789 ± 0.037
0.371ProCys: 0.371 ± 0.025
0.891ProAsp: 0.891 ± 0.038
1.583ProGlu: 1.583 ± 0.047
1.345ProPhe: 1.345 ± 0.045
0.87ProGly: 0.87 ± 0.045
0.401ProHis: 0.401 ± 0.021
1.547ProIle: 1.547 ± 0.053
1.887ProLys: 1.887 ± 0.054
1.83ProLeu: 1.83 ± 0.06
0.415ProMet: 0.415 ± 0.024
1.377ProAsn: 1.377 ± 0.044
0.545ProPro: 0.545 ± 0.034
0.587ProGln: 0.587 ± 0.032
0.577ProArg: 0.577 ± 0.027
1.486ProSer: 1.486 ± 0.051
1.029ProThr: 1.029 ± 0.05
1.424ProVal: 1.424 ± 0.049
0.1ProTrp: 0.1 ± 0.012
0.929ProTyr: 0.929 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
0.829GlnAla: 0.829 ± 0.036
0.41GlnCys: 0.41 ± 0.023
1.034GlnAsp: 1.034 ± 0.042
2.132GlnGlu: 2.132 ± 0.067
1.25GlnPhe: 1.25 ± 0.046
0.867GlnGly: 0.867 ± 0.035
0.502GlnHis: 0.502 ± 0.023
2.685GlnIle: 2.685 ± 0.063
3.303GlnLys: 3.303 ± 0.069
2.136GlnLeu: 2.136 ± 0.059
0.842GlnMet: 0.842 ± 0.032
2.49GlnAsn: 2.49 ± 0.06
0.588GlnPro: 0.588 ± 0.029
1.056GlnGln: 1.056 ± 0.055
0.932GlnArg: 0.932 ± 0.037
1.393GlnSer: 1.393 ± 0.045
1.579GlnThr: 1.579 ± 0.052
1.221GlnVal: 1.221 ± 0.044
0.146GlnTrp: 0.146 ± 0.016
1.005GlnTyr: 1.005 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
1.065ArgAla: 1.065 ± 0.043
0.541ArgCys: 0.541 ± 0.032
1.281ArgAsp: 1.281 ± 0.043
2.37ArgGlu: 2.37 ± 0.071
1.513ArgPhe: 1.513 ± 0.044
1.028ArgGly: 1.028 ± 0.041
0.591ArgHis: 0.591 ± 0.029
2.734ArgIle: 2.734 ± 0.061
3.535ArgLys: 3.535 ± 0.082
2.317ArgLeu: 2.317 ± 0.055
0.778ArgMet: 0.778 ± 0.037
2.491ArgAsn: 2.491 ± 0.06
0.646ArgPro: 0.646 ± 0.035
0.807ArgGln: 0.807 ± 0.029
1.342ArgArg: 1.342 ± 0.078
1.423ArgSer: 1.423 ± 0.048
1.296ArgThr: 1.296 ± 0.04
1.442ArgVal: 1.442 ± 0.045
0.185ArgTrp: 0.185 ± 0.015
1.262ArgTyr: 1.262 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
2.128SerAla: 2.128 ± 0.066
1.152SerCys: 1.152 ± 0.041
3.577SerAsp: 3.577 ± 0.072
4.381SerGlu: 4.381 ± 0.08
4.293SerPhe: 4.293 ± 0.092
2.378SerGly: 2.378 ± 0.066
1.059SerHis: 1.059 ± 0.038
5.656SerIle: 5.656 ± 0.088
6.769SerLys: 6.769 ± 0.117
5.655SerLeu: 5.655 ± 0.092
1.3SerMet: 1.3 ± 0.04
6.719SerAsn: 6.719 ± 0.186
1.168SerPro: 1.168 ± 0.051
1.656SerGln: 1.656 ± 0.049
1.608SerArg: 1.608 ± 0.047
4.844SerSer: 4.844 ± 0.141
3.299SerThr: 3.299 ± 0.073
3.335SerVal: 3.335 ± 0.071
0.26SerTrp: 0.26 ± 0.018
2.6SerTyr: 2.6 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
1.925ThrAla: 1.925 ± 0.055
1.001ThrCys: 1.001 ± 0.038
2.973ThrAsp: 2.973 ± 0.07
3.822ThrGlu: 3.822 ± 0.089
2.931ThrPhe: 2.931 ± 0.065
2.174ThrGly: 2.174 ± 0.062
1.123ThrHis: 1.123 ± 0.039
4.193ThrIle: 4.193 ± 0.083
5.263ThrLys: 5.263 ± 0.09
4.557ThrLeu: 4.557 ± 0.094
0.897ThrMet: 0.897 ± 0.032
6.553ThrAsn: 6.553 ± 0.219
1.297ThrPro: 1.297 ± 0.05
1.567ThrGln: 1.567 ± 0.052
1.459ThrArg: 1.459 ± 0.062
3.176ThrSer: 3.176 ± 0.071
2.831ThrThr: 2.831 ± 0.079
2.85ThrVal: 2.85 ± 0.07
0.268ThrTrp: 0.268 ± 0.02
2.598ThrTyr: 2.598 ± 0.097
0.0ThrXaa: 0.0 ± 0.0
Val
1.818ValAla: 1.818 ± 0.049
1.163ValCys: 1.163 ± 0.041
3.02ValAsp: 3.02 ± 0.07
4.146ValGlu: 4.146 ± 0.099
4.116ValPhe: 4.116 ± 0.092
1.97ValGly: 1.97 ± 0.056
0.867ValHis: 0.867 ± 0.035
4.073ValIle: 4.073 ± 0.085
5.5ValLys: 5.5 ± 0.079
5.241ValLeu: 5.241 ± 0.086
1.064ValMet: 1.064 ± 0.045
3.623ValAsn: 3.623 ± 0.068
1.187ValPro: 1.187 ± 0.043
1.284ValGln: 1.284 ± 0.051
1.417ValArg: 1.417 ± 0.05
3.447ValSer: 3.447 ± 0.073
2.246ValThr: 2.246 ± 0.065
3.141ValVal: 3.141 ± 0.074
0.264ValTrp: 0.264 ± 0.018
2.538ValTyr: 2.538 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.217TrpAla: 0.217 ± 0.018
0.115TrpCys: 0.115 ± 0.013
0.247TrpAsp: 0.247 ± 0.02
0.328TrpGlu: 0.328 ± 0.024
0.378TrpPhe: 0.378 ± 0.023
0.21TrpGly: 0.21 ± 0.018
0.108TrpHis: 0.108 ± 0.011
0.441TrpIle: 0.441 ± 0.025
0.409TrpLys: 0.409 ± 0.024
0.446TrpLeu: 0.446 ± 0.027
0.117TrpMet: 0.117 ± 0.013
0.378TrpAsn: 0.378 ± 0.022
0.136TrpPro: 0.136 ± 0.014
0.108TrpGln: 0.108 ± 0.011
0.159TrpArg: 0.159 ± 0.017
0.325TrpSer: 0.325 ± 0.021
0.212TrpThr: 0.212 ± 0.018
0.283TrpVal: 0.283 ± 0.021
0.032TrpTrp: 0.032 ± 0.007
0.236TrpTyr: 0.236 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.69TyrAla: 1.69 ± 0.05
0.724TyrCys: 0.724 ± 0.036
2.925TyrAsp: 2.925 ± 0.078
2.909TyrGlu: 2.909 ± 0.068
3.013TyrPhe: 3.013 ± 0.069
1.798TyrGly: 1.798 ± 0.076
0.67TyrHis: 0.67 ± 0.03
4.186TyrIle: 4.186 ± 0.135
5.42TyrLys: 5.42 ± 0.101
4.095TyrLeu: 4.095 ± 0.087
1.038TyrMet: 1.038 ± 0.042
5.496TyrAsn: 5.496 ± 0.198
0.713TyrPro: 0.713 ± 0.039
0.891TyrGln: 0.891 ± 0.039
1.307TyrArg: 1.307 ± 0.039
2.642TyrSer: 2.642 ± 0.057
2.587TyrThr: 2.587 ± 0.062
2.577TyrVal: 2.577 ± 0.056
0.204TyrTrp: 0.204 ± 0.017
2.028TyrTyr: 2.028 ± 0.085
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2431 proteins (741464 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski