Hydrocarboniphaga daqingensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Hydrocarboniphaga

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5LZQ8|A0A1M5LZQ8_9GAMM NAD(P)-dependent dehydrogenase short-chain alcohol dehydrogenase family OS=Hydrocarboniphaga daqingensis OX=490188 GN=SAMN04488068_1170 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 9.68MHH4 pKa = 6.6YY5 pKa = 9.35LAAAAAVFGCTTASAATLADD25 pKa = 3.63LAKK28 pKa = 10.36EE29 pKa = 4.25VTASGWISGSYY40 pKa = 10.2AYY42 pKa = 10.79NFNDD46 pKa = 4.1SNGLIQGRR54 pKa = 11.84VADD57 pKa = 3.91VQADD61 pKa = 3.99SFVLNQAVLNLSSAPTEE78 pKa = 4.36GFGGTVMLLAGEE90 pKa = 4.46DD91 pKa = 3.46AANVINPSYY100 pKa = 11.33GDD102 pKa = 3.57TDD104 pKa = 3.81DD105 pKa = 5.11KK106 pKa = 11.58LAVQQAYY113 pKa = 10.45LSYY116 pKa = 10.85AAGDD120 pKa = 3.64LTVIAGRR127 pKa = 11.84YY128 pKa = 8.48NSLAGYY134 pKa = 8.47EE135 pKa = 4.19VLADD139 pKa = 4.06PSNTTLSRR147 pKa = 11.84SLVYY151 pKa = 10.45INAQPYY157 pKa = 8.03FHH159 pKa = 6.72TGVRR163 pKa = 11.84AAYY166 pKa = 8.63KK167 pKa = 10.6ASDD170 pKa = 3.43AATLFLGLANSAALGASVDD189 pKa = 3.79ANDD192 pKa = 4.18PKK194 pKa = 10.53TIEE197 pKa = 3.99VGGLFTLSPAVTVGIYY213 pKa = 10.21DD214 pKa = 3.71YY215 pKa = 11.17FGVEE219 pKa = 4.8AGDD222 pKa = 3.43TGKK225 pKa = 8.52NTNYY229 pKa = 10.57LDD231 pKa = 3.89FVGTLTATDD240 pKa = 3.74TLSFAINADD249 pKa = 3.64YY250 pKa = 10.44FSQEE254 pKa = 3.83DD255 pKa = 4.05TVDD258 pKa = 3.56VVGVAGYY265 pKa = 7.61ATLKK269 pKa = 10.82LNDD272 pKa = 3.38MWSATVRR279 pKa = 11.84LEE281 pKa = 4.1NVNIEE286 pKa = 4.03PDD288 pKa = 3.39AGSDD292 pKa = 3.46LTINEE297 pKa = 3.88ATLALTYY304 pKa = 10.88APASNFKK311 pKa = 9.78MFFEE315 pKa = 4.7GRR317 pKa = 11.84VDD319 pKa = 4.85DD320 pKa = 5.3ADD322 pKa = 4.01ADD324 pKa = 3.84IYY326 pKa = 10.93IDD328 pKa = 3.69GTDD331 pKa = 3.81FTGTQPTLGVKK342 pKa = 10.36AIYY345 pKa = 9.12TFGMM349 pKa = 3.94

Molecular weight: 36.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5LJC7|A0A1M5LJC7_9GAMM Exopolyphosphatase / guanosine-5'-triphosphate 3'-diphosphate pyrophosphatase OS=Hydrocarboniphaga daqingensis OX=490188 GN=SAMN04488068_0930 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.54RR3 pKa = 11.84TFQPHH8 pKa = 4.47SLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.45RR14 pKa = 11.84THH16 pKa = 6.03GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.32GRR39 pKa = 11.84ARR41 pKa = 11.84LTPVV45 pKa = 2.71

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3253

0

3253

1160950

28

3144

356.9

38.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.608 ± 0.059

0.886 ± 0.012

6.016 ± 0.035

4.862 ± 0.037

3.29 ± 0.026

8.208 ± 0.046

2.075 ± 0.022

4.566 ± 0.028

2.717 ± 0.038

10.904 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.169 ± 0.021

2.607 ± 0.027

5.379 ± 0.033

4.04 ± 0.026

7.552 ± 0.045

5.661 ± 0.036

5.176 ± 0.038

7.375 ± 0.034

1.386 ± 0.018

2.523 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski