Pseudorhodoferax sp. Leaf274

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Pseudorhodoferax; unclassified Pseudorhodoferax

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9NTP8|A0A0S9NTP8_9BURK Alpha/beta hydrolase OS=Pseudorhodoferax sp. Leaf274 OX=1736318 GN=ASF44_22270 PE=4 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.62NIEE9 pKa = 4.24VMPDD13 pKa = 3.7PIVFTDD19 pKa = 3.89AAAAKK24 pKa = 9.92VADD27 pKa = 5.47LIAEE31 pKa = 4.29EE32 pKa = 4.95GNPEE36 pKa = 3.75LKK38 pKa = 10.68LRR40 pKa = 11.84VFVQGGGCSGFSYY53 pKa = 10.72GFTFDD58 pKa = 4.5EE59 pKa = 5.2ISNEE63 pKa = 4.38DD64 pKa = 3.86DD65 pKa = 3.09TTMTKK70 pKa = 10.66NGVSLLIDD78 pKa = 3.54AMSYY82 pKa = 10.32QYY84 pKa = 11.5LVGAEE89 pKa = 3.79IDD91 pKa = 3.91YY92 pKa = 11.36KK93 pKa = 11.19EE94 pKa = 4.67DD95 pKa = 3.23LQGAQFVIKK104 pKa = 10.4NPNATTTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9NWR9|A0A0S9NWR9_9BURK Carnitine dehydratase OS=Pseudorhodoferax sp. Leaf274 OX=1736318 GN=ASF44_03655 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 8.78IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.42GFLARR21 pKa = 11.84MKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5992

0

5992

2002829

32

7141

334.3

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.366 ± 0.048

0.886 ± 0.009

5.181 ± 0.023

4.858 ± 0.027

3.331 ± 0.017

8.707 ± 0.032

2.253 ± 0.017

3.789 ± 0.022

2.66 ± 0.027

11.075 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.015

2.286 ± 0.019

5.502 ± 0.024

4.294 ± 0.021

7.254 ± 0.035

4.846 ± 0.02

4.983 ± 0.029

7.777 ± 0.026

1.489 ± 0.015

2.104 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski