Sediminicola sp. YIK13

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Sediminicola; unclassified Sediminicola

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S1SDI8|A0A0S1SDI8_9FLAO X-Pro aminopeptidase OS=Sediminicola sp. YIK13 OX=1453352 GN=SB49_11605 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.37KK3 pKa = 9.61ILLKK7 pKa = 10.53ISIVALALITLGCSSEE23 pKa = 4.84DD24 pKa = 3.45NVIDD28 pKa = 3.58IVSRR32 pKa = 11.84DD33 pKa = 3.82LSTEE37 pKa = 3.99LPFLRR42 pKa = 11.84ITSQTGRR49 pKa = 11.84SLNLFDD55 pKa = 4.48TEE57 pKa = 3.98STYY60 pKa = 10.76TVNFEE65 pKa = 4.09YY66 pKa = 11.06QEE68 pKa = 4.19VEE70 pKa = 3.8NTAVMDD76 pKa = 3.27QFEE79 pKa = 4.46RR80 pKa = 11.84VDD82 pKa = 3.81FTINFIDD89 pKa = 3.83NFEE92 pKa = 5.04DD93 pKa = 4.55GVDD96 pKa = 3.5NSKK99 pKa = 11.0AAVSLGSLSKK109 pKa = 10.89SDD111 pKa = 4.56FSALSSFGMPTSSFDD126 pKa = 3.57YY127 pKa = 10.63TFGEE131 pKa = 4.33ALTALGLTTAQVNGGDD147 pKa = 3.44QFQLNWEE154 pKa = 4.34LFLTDD159 pKa = 3.94GSSINSSDD167 pKa = 3.38VSGNISAVGGYY178 pKa = 9.74YY179 pKa = 10.14SAPYY183 pKa = 9.5QSRR186 pKa = 11.84AAMVCLFDD194 pKa = 4.77APEE197 pKa = 4.17FFVGDD202 pKa = 3.7YY203 pKa = 10.94QMEE206 pKa = 4.39QLTGADD212 pKa = 3.67PFYY215 pKa = 11.27GDD217 pKa = 3.86EE218 pKa = 4.3TFGTQVVTLTATGATQRR235 pKa = 11.84QFNFLYY241 pKa = 10.8YY242 pKa = 10.06PGNFDD247 pKa = 3.5TNYY250 pKa = 8.39TFTMNFVCDD259 pKa = 4.71RR260 pKa = 11.84ISMVGTGGLGCGGSIGQTTGDD281 pKa = 3.51TPTFFDD287 pKa = 4.17QNLVDD292 pKa = 4.14DD293 pKa = 5.05AEE295 pKa = 5.79IILSITDD302 pKa = 3.69FDD304 pKa = 5.05GDD306 pKa = 4.18GGCGTGSNQITVRR319 pKa = 11.84LTKK322 pKa = 10.68LL323 pKa = 3.06

Molecular weight: 34.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S1SA06|A0A0S1SA06_9FLAO Endoribonuclease L-PSP OS=Sediminicola sp. YIK13 OX=1453352 GN=SB49_01325 PE=4 SV=1
MM1 pKa = 7.33VLRR4 pKa = 11.84KK5 pKa = 9.65GCRR8 pKa = 11.84FAARR12 pKa = 11.84NNEE15 pKa = 3.74GCGGKK20 pKa = 9.67NGRR23 pKa = 11.84KK24 pKa = 9.1AVFIEE29 pKa = 4.43GSGAAKK35 pKa = 10.14EE36 pKa = 3.98KK37 pKa = 10.22RR38 pKa = 11.84KK39 pKa = 10.54KK40 pKa = 10.7FFIFYY45 pKa = 10.51LRR47 pKa = 11.84GTKK50 pKa = 9.6RR51 pKa = 11.84VVYY54 pKa = 7.85LHH56 pKa = 6.49PLWEE60 pKa = 4.27TEE62 pKa = 3.98RR63 pKa = 11.84QNKK66 pKa = 8.26NKK68 pKa = 9.46NRR70 pKa = 11.84QVLL73 pKa = 3.59

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2966

0

2966

1011104

50

5634

340.9

38.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.574 ± 0.046

0.691 ± 0.016

5.647 ± 0.052

6.701 ± 0.05

5.077 ± 0.038

6.802 ± 0.048

1.813 ± 0.023

7.72 ± 0.042

7.552 ± 0.074

9.522 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.025

5.714 ± 0.049

3.497 ± 0.029

3.342 ± 0.024

3.518 ± 0.037

6.348 ± 0.036

5.749 ± 0.075

6.35 ± 0.036

1.046 ± 0.018

3.938 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski