Kigluaik phantom orthophasmavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phasmaviridae; Orthophasmavirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059XT43|A0A059XT43_9VIRU Nucleoprotein OS=Kigluaik phantom orthophasmavirus OX=1980539 GN=N PE=4 SV=1
MM1 pKa = 6.8KK2 pKa = 10.46QKK4 pKa = 10.4IGTNMLMFLFFYY16 pKa = 10.66NIIIAEE22 pKa = 4.44GSHH25 pKa = 6.3GPNIYY30 pKa = 10.29SKK32 pKa = 10.34NGEE35 pKa = 4.21VEE37 pKa = 4.48FIGYY41 pKa = 8.4DD42 pKa = 3.2EE43 pKa = 5.49CSISVNKK50 pKa = 9.54MVINSTNGIFPGIHH64 pKa = 6.51FGLIEE69 pKa = 4.1YY70 pKa = 8.82TCDD73 pKa = 3.65KK74 pKa = 9.74ITGKK78 pKa = 10.22IISRR82 pKa = 11.84MSCMEE87 pKa = 3.47CRR89 pKa = 11.84FYY91 pKa = 10.69CTHH94 pKa = 5.98NQNIVEE100 pKa = 4.25CSNYY104 pKa = 10.09YY105 pKa = 9.91IPLISGFSVGAIFMIILIYY124 pKa = 10.49VLSKK128 pKa = 11.13NDD130 pKa = 3.21VLKK133 pKa = 10.84RR134 pKa = 11.84ISNSLEE140 pKa = 3.63NNVISKK146 pKa = 10.58LSSRR150 pKa = 11.84KK151 pKa = 9.38SKK153 pKa = 10.23NARR156 pKa = 11.84RR157 pKa = 11.84LLKK160 pKa = 10.29KK161 pKa = 10.23IRR163 pKa = 11.84DD164 pKa = 3.51AEE166 pKa = 4.53AINNSNIKK174 pKa = 9.79MIGSTGQEE182 pKa = 4.43DD183 pKa = 3.57IDD185 pKa = 4.88LHH187 pKa = 8.35NEE189 pKa = 3.95DD190 pKa = 4.75DD191 pKa = 4.07NKK193 pKa = 11.45GIMKK197 pKa = 10.42RR198 pKa = 11.84GLNVEE203 pKa = 4.33ASNDD207 pKa = 2.9IDD209 pKa = 4.22IGSNSNGHH217 pKa = 6.24LFYY220 pKa = 11.23YY221 pKa = 9.43EE222 pKa = 4.66HH223 pKa = 7.09EE224 pKa = 4.79PEE226 pKa = 5.82AIEE229 pKa = 3.96YY230 pKa = 9.47KK231 pKa = 10.4RR232 pKa = 11.84RR233 pKa = 11.84GSVPRR238 pKa = 11.84NVTILSMIIMLSIPPLILGCDD259 pKa = 2.87NTFFLNSEE267 pKa = 4.39GKK269 pKa = 8.65VCDD272 pKa = 4.26YY273 pKa = 10.87KK274 pKa = 11.39GCQNKK279 pKa = 9.24NFVSMPLMKK288 pKa = 10.46GNRR291 pKa = 11.84ICVRR295 pKa = 11.84DD296 pKa = 3.48SDD298 pKa = 4.19QNILAISITEE308 pKa = 3.82SNIIYY313 pKa = 9.71RR314 pKa = 11.84HH315 pKa = 5.51HH316 pKa = 7.29LMYY319 pKa = 10.16YY320 pKa = 6.19TTDD323 pKa = 3.33YY324 pKa = 11.09EE325 pKa = 4.48IDD327 pKa = 3.85VKK329 pKa = 11.2HH330 pKa = 5.92HH331 pKa = 5.66TNCLGAGKK339 pKa = 10.12CDD341 pKa = 3.54EE342 pKa = 4.67EE343 pKa = 4.39KK344 pKa = 11.1CNLISDD350 pKa = 4.64EE351 pKa = 4.41YY352 pKa = 11.47KK353 pKa = 11.27GMIPNNSVSGNGCSEE368 pKa = 3.86GFDD371 pKa = 3.43RR372 pKa = 11.84CSNMCFYY379 pKa = 11.0SVSCTFYY386 pKa = 10.61SWYY389 pKa = 10.56LKK391 pKa = 10.12EE392 pKa = 5.04GKK394 pKa = 9.2IKK396 pKa = 10.71YY397 pKa = 8.73PVYY400 pKa = 10.66TMSSQNWEE408 pKa = 3.54VSIAFEE414 pKa = 4.15YY415 pKa = 10.84LGARR419 pKa = 11.84YY420 pKa = 9.45IKK422 pKa = 10.38ILDD425 pKa = 3.55VNNPDD430 pKa = 3.22GSIMINGKK438 pKa = 7.28EE439 pKa = 3.95VKK441 pKa = 10.03IGISSFTSSSIRR453 pKa = 11.84LEE455 pKa = 3.88RR456 pKa = 11.84DD457 pKa = 3.16SIIINNTLYY466 pKa = 11.12LVDD469 pKa = 4.15SSFANMPEE477 pKa = 3.75TDD479 pKa = 4.58KK480 pKa = 11.5IGDD483 pKa = 3.64YY484 pKa = 10.83QLAIDD489 pKa = 4.54GKK491 pKa = 8.53SRR493 pKa = 11.84VYY495 pKa = 8.61NTHH498 pKa = 7.16NIRR501 pKa = 11.84CQTDD505 pKa = 3.78FCKK508 pKa = 8.48TTCASPEE515 pKa = 3.78PKK517 pKa = 9.59INRR520 pKa = 11.84FISSIEE526 pKa = 3.9RR527 pKa = 11.84YY528 pKa = 9.54RR529 pKa = 11.84SLKK532 pKa = 9.73FSKK535 pKa = 10.07IGNSNNINVHH545 pKa = 5.43EE546 pKa = 4.48PVNGLITMNIGDD558 pKa = 4.06IRR560 pKa = 11.84IEE562 pKa = 3.96SLQVIDD568 pKa = 4.69ASCDD572 pKa = 3.07IEE574 pKa = 5.84VMMTYY579 pKa = 10.31SCVGCDD585 pKa = 3.07VDD587 pKa = 5.37SYY589 pKa = 12.08AVVQAYY595 pKa = 9.71NIKK598 pKa = 10.09QSGIIPITSTCNLVKK613 pKa = 10.44DD614 pKa = 4.38YY615 pKa = 10.71ISCEE619 pKa = 3.69DD620 pKa = 4.22TINKK624 pKa = 9.35IEE626 pKa = 4.19FVDD629 pKa = 3.78NSKK632 pKa = 10.39VCYY635 pKa = 10.11ISMKK639 pKa = 10.03GRR641 pKa = 11.84NDD643 pKa = 3.62TIKK646 pKa = 10.7IMFNTTYY653 pKa = 9.91LGSMDD658 pKa = 4.08PSKK661 pKa = 10.94SIYY664 pKa = 9.03ATSSRR669 pKa = 11.84MDD671 pKa = 5.38DD672 pKa = 2.83ITGLVTSSGFINGIISTIGSISLFTIIGTFATRR705 pKa = 11.84LIGVILTMRR714 pKa = 11.84SVDD717 pKa = 3.46KK718 pKa = 10.87EE719 pKa = 4.42SYY721 pKa = 9.92RR722 pKa = 11.84SNAIII727 pKa = 4.38

Molecular weight: 81.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059XT43|A0A059XT43_9VIRU Nucleoprotein OS=Kigluaik phantom orthophasmavirus OX=1980539 GN=N PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 4.9KK3 pKa = 10.51PSEE6 pKa = 4.16AFTTAEE12 pKa = 3.58NSMVGLNLEE21 pKa = 4.54GPVQEE26 pKa = 4.67AVRR29 pKa = 11.84KK30 pKa = 7.5TFDD33 pKa = 3.16MHH35 pKa = 6.89AKK37 pKa = 9.55TIATMASHH45 pKa = 7.09PCSRR49 pKa = 11.84DD50 pKa = 3.02YY51 pKa = 12.1NMFLNTCEE59 pKa = 4.45SVSKK63 pKa = 9.98EE64 pKa = 3.98YY65 pKa = 11.25NSAGLDD71 pKa = 3.49AEE73 pKa = 4.39KK74 pKa = 10.62LCRR77 pKa = 11.84EE78 pKa = 3.97MSYY81 pKa = 11.33VNPDD85 pKa = 3.09FMLEE89 pKa = 4.1PGWEE93 pKa = 4.21VGHH96 pKa = 6.22QALNFMAKK104 pKa = 10.12VIFEE108 pKa = 4.39VGPEE112 pKa = 3.88TRR114 pKa = 11.84SVKK117 pKa = 10.53VGSGTDD123 pKa = 2.78KK124 pKa = 10.42SYY126 pKa = 11.04RR127 pKa = 11.84FRR129 pKa = 11.84FVRR132 pKa = 11.84QYY134 pKa = 11.23DD135 pKa = 3.29AADD138 pKa = 3.31IGRR141 pKa = 11.84VASKK145 pKa = 9.68MGAKK149 pKa = 9.89NPPNAPSTSAARR161 pKa = 11.84PNAVDD166 pKa = 3.45VVTAYY171 pKa = 9.63KK172 pKa = 10.68AKK174 pKa = 9.91IRR176 pKa = 11.84EE177 pKa = 4.03HH178 pKa = 7.17SVEE181 pKa = 3.97NGEE184 pKa = 4.18AGTVVSGMVIVKK196 pKa = 8.77TYY198 pKa = 10.61KK199 pKa = 9.47NTIAPQSEE207 pKa = 4.11PTVKK211 pKa = 10.68NGVLVLTIKK220 pKa = 10.31QASFLALYY228 pKa = 9.9KK229 pKa = 10.49FKK231 pKa = 11.01EE232 pKa = 4.22FVKK235 pKa = 10.6VCVARR240 pKa = 11.84DD241 pKa = 3.77TTILTPLCGAIFSRR255 pKa = 11.84DD256 pKa = 4.07DD257 pKa = 3.59IDD259 pKa = 6.19LMLADD264 pKa = 4.83PEE266 pKa = 4.21IKK268 pKa = 10.45AVITDD273 pKa = 3.56KK274 pKa = 11.48TEE276 pKa = 4.04LLVALNLSAQNGGQFVEE293 pKa = 5.18GSRR296 pKa = 11.84TDD298 pKa = 3.12IAGVCAIMGTVNVEE312 pKa = 3.78KK313 pKa = 10.4KK314 pKa = 10.67LRR316 pKa = 11.84MQIISKK322 pKa = 7.34TAKK325 pKa = 9.81QFLAKK330 pKa = 10.07DD331 pKa = 3.39RR332 pKa = 11.84PMDD335 pKa = 3.76KK336 pKa = 11.03GLFHH340 pKa = 7.49AVGKK344 pKa = 9.64YY345 pKa = 9.8AAGGLPAEE353 pKa = 4.36WQAEE357 pKa = 4.05ILVQEE362 pKa = 4.59LEE364 pKa = 4.18AAKK367 pKa = 8.82ITYY370 pKa = 9.63RR371 pKa = 11.84QILAAKK377 pKa = 9.28KK378 pKa = 8.07ATEE381 pKa = 3.64RR382 pKa = 11.84AIRR385 pKa = 11.84MEE387 pKa = 4.12VPNFDD392 pKa = 3.4GSEE395 pKa = 3.82NN396 pKa = 3.52

Molecular weight: 43.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3254

396

2131

1084.7

123.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.948 ± 1.552

1.967 ± 0.616

6.146 ± 0.522

6.361 ± 0.473

3.749 ± 0.072

5.47 ± 0.589

1.936 ± 0.249

8.543 ± 1.686

6.976 ± 0.306

7.468 ± 0.926

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.473 ± 0.153

7.037 ± 0.686

3.258 ± 0.361

2.612 ± 0.415

4.948 ± 0.445

7.99 ± 1.071

5.655 ± 0.224

5.747 ± 0.745

0.707 ± 0.204

5.009 ± 0.665
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski