Mucilaginibacter limnophilus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437MR87|A0A437MR87_9SPHI Response regulator OS=Mucilaginibacter limnophilus OX=1932778 GN=EOD41_14445 PE=4 SV=1
MM1 pKa = 7.16STLPNDD7 pKa = 4.01PPGSIPVDD15 pKa = 3.27IASQWTANWRR25 pKa = 11.84EE26 pKa = 4.25FIASSNQAFNILAFTIPIEE45 pKa = 4.57CIKK48 pKa = 11.23NMLLYY53 pKa = 10.72NEE55 pKa = 4.11NAEE58 pKa = 4.5GIRR61 pKa = 11.84AYY63 pKa = 9.72IALEE67 pKa = 3.98NADD70 pKa = 4.0EE71 pKa = 4.2VSSAKK76 pKa = 10.47LVMVPVIGNEE86 pKa = 4.32DD87 pKa = 4.41IINQPADD94 pKa = 3.33NSKK97 pKa = 11.44SNVYY101 pKa = 10.68DD102 pKa = 3.55NTDD105 pKa = 2.98ACPPVCALNSPLNN118 pKa = 3.71

Molecular weight: 12.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A437MFN4|A0A437MFN4_9SPHI Zinc/iron permease OS=Mucilaginibacter limnophilus OX=1932778 GN=EOD41_20360 PE=4 SV=1
MM1 pKa = 7.58SNAQVAFFIGLFGSIHH17 pKa = 7.0CIGMCGPLAFAVPFTGNSNLLLVWDD42 pKa = 4.18KK43 pKa = 11.42LIYY46 pKa = 9.32QAGRR50 pKa = 11.84VISYY54 pKa = 10.35GVLGLIAGLIGKK66 pKa = 7.14QLWMAGLQQGISIASGVLIIMAAASRR92 pKa = 11.84LFKK95 pKa = 10.32WRR97 pKa = 11.84MAVSRR102 pKa = 11.84LDD104 pKa = 3.15VFAPFNRR111 pKa = 11.84LLGYY115 pKa = 10.02ALKK118 pKa = 10.94NRR120 pKa = 11.84MGHH123 pKa = 6.65LFIGMLNGLLPCGFVYY139 pKa = 10.52LALVGAINTGSVTSSAFFMLWFGAGTVPLMLAATLGAGLLTPLVRR184 pKa = 11.84TRR186 pKa = 11.84ISRR189 pKa = 11.84AMPYY193 pKa = 10.66FMLFLGVWFILRR205 pKa = 11.84GLALDD210 pKa = 4.03IPYY213 pKa = 10.17ISPAKK218 pKa = 10.4SADD221 pKa = 3.36TAICHH226 pKa = 6.09

Molecular weight: 24.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4021

0

4021

1373873

25

2634

341.7

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.675 ± 0.035

0.786 ± 0.012

5.436 ± 0.025

5.617 ± 0.039

4.865 ± 0.03

6.867 ± 0.038

1.877 ± 0.021

7.127 ± 0.033

6.848 ± 0.034

9.33 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.018

5.675 ± 0.035

3.905 ± 0.022

3.745 ± 0.026

4.101 ± 0.025

6.031 ± 0.025

5.863 ± 0.034

6.545 ± 0.029

1.221 ± 0.015

4.271 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski