Roseobacter phage CRP-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QWW1|A0A646QWW1_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-2 OX=2559281 GN=CRP2_gp36 PE=4 SV=1
MM1 pKa = 7.5YY2 pKa = 10.4INNKK6 pKa = 9.58KK7 pKa = 10.11FDD9 pKa = 3.93SDD11 pKa = 3.36LDD13 pKa = 3.66AVYY16 pKa = 10.46DD17 pKa = 4.15GSHH20 pKa = 6.17IEE22 pKa = 4.09SWDD25 pKa = 3.26YY26 pKa = 10.75WSQGDD31 pKa = 3.63VV32 pKa = 3.09

Molecular weight: 3.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QVQ4|A0A646QVQ4_9CAUD Uncharacterized protein OS=Roseobacter phage CRP-2 OX=2559281 GN=CRP2_gp15 PE=4 SV=1
MM1 pKa = 7.01KK2 pKa = 9.91QKK4 pKa = 9.89TMIRR8 pKa = 11.84MMNPRR13 pKa = 11.84TGLGRR18 pKa = 11.84YY19 pKa = 6.66VKK21 pKa = 10.27RR22 pKa = 11.84VATAISVTVNVILGGSNNQTFSARR46 pKa = 11.84NHH48 pKa = 4.72QWRR51 pKa = 11.84KK52 pKa = 9.79DD53 pKa = 3.49GKK55 pKa = 8.71PNIVFLIDD63 pKa = 3.86LFLGKK68 pKa = 8.83GHH70 pKa = 7.08CLEE73 pKa = 4.69CWVYY77 pKa = 10.07WKK79 pKa = 8.98TRR81 pKa = 11.84RR82 pKa = 11.84KK83 pKa = 7.66WW84 pKa = 3.1

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

17216

32

1222

235.8

26.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.282 ± 0.538

0.807 ± 0.154

6.715 ± 0.22

6.529 ± 0.534

3.462 ± 0.18

7.278 ± 0.439

1.626 ± 0.207

5.106 ± 0.194

6.186 ± 0.503

6.837 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.19

4.978 ± 0.272

3.793 ± 0.297

4.31 ± 0.503

4.397 ± 0.316

6.36 ± 0.5

7.388 ± 0.68

6.709 ± 0.218

1.324 ± 0.144

3.863 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski