Amino acid dipepetide frequency for Poecilia formosa (Amazon molly) (Limia formosa)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.622AlaAla: 6.622 ± 0.033	1.278AlaCys: 1.278 ± 0.011	3.246AlaAsp: 3.246 ± 0.015	4.727AlaGlu: 4.727 ± 0.024	2.361AlaPhe: 2.361 ± 0.013	4.399AlaGly: 4.399 ± 0.019	1.428AlaHis: 1.428 ± 0.012	2.552AlaIle: 2.552 ± 0.015	3.341AlaLys: 3.341 ± 0.021	6.383AlaLeu: 6.383 ± 0.024	1.509AlaMet: 1.509 ± 0.01	2.14AlaAsn: 2.14 ± 0.012	3.52AlaPro: 3.52 ± 0.02	2.868AlaGln: 2.868 ± 0.017	3.009AlaArg: 3.009 ± 0.015	5.502AlaSer: 5.502 ± 0.024	3.396AlaThr: 3.396 ± 0.017	4.908AlaVal: 4.908 ± 0.02	0.631AlaTrp: 0.631 ± 0.008	1.452AlaTyr: 1.452 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.155CysAla: 1.155 ± 0.009	0.681CysCys: 0.681 ± 0.009	1.174CysAsp: 1.174 ± 0.013	1.319CysGlu: 1.319 ± 0.013	0.924CysPhe: 0.924 ± 0.008	1.723CysGly: 1.723 ± 0.02	0.642CysHis: 0.642 ± 0.008	0.964CysIle: 0.964 ± 0.01	1.198CysLys: 1.198 ± 0.01	2.2CysLeu: 2.2 ± 0.016	0.456CysMet: 0.456 ± 0.005	0.857CysAsn: 0.857 ± 0.008	1.314CysPro: 1.314 ± 0.015	1.056CysGln: 1.056 ± 0.011	1.357CysArg: 1.357 ± 0.012	2.346CysSer: 2.346 ± 0.019	1.15CysThr: 1.15 ± 0.01	1.514CysVal: 1.514 ± 0.013	0.304CysTrp: 0.304 ± 0.004	0.622CysTyr: 0.622 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	2.971AspAla: 2.971 ± 0.014	1.165AspCys: 1.165 ± 0.011	3.14AspAsp: 3.14 ± 0.019	3.859AspGlu: 3.859 ± 0.021	2.144AspPhe: 2.144 ± 0.014	3.785AspGly: 3.785 ± 0.021	1.164AspHis: 1.164 ± 0.009	2.562AspIle: 2.562 ± 0.019	2.705AspLys: 2.705 ± 0.016	5.019AspLeu: 5.019 ± 0.022	1.231AspMet: 1.231 ± 0.01	1.876AspAsn: 1.876 ± 0.013	2.874AspPro: 2.874 ± 0.015	2.04AspGln: 2.04 ± 0.01	2.794AspArg: 2.794 ± 0.019	4.539AspSer: 4.539 ± 0.02	2.457AspThr: 2.457 ± 0.011	3.376AspVal: 3.376 ± 0.018	0.688AspTrp: 0.688 ± 0.007	1.489AspTyr: 1.489 ± 0.01	0.001AspXaa: 0.001 ± 0.0
Glu	4.721GluAla: 4.721 ± 0.023	1.198GluCys: 1.198 ± 0.012	4.459GluAsp: 4.459 ± 0.02	7.74GluGlu: 7.74 ± 0.05	1.976GluPhe: 1.976 ± 0.011	3.961GluGly: 3.961 ± 0.022	1.417GluHis: 1.417 ± 0.01	2.862GluIle: 2.862 ± 0.017	4.909GluLys: 4.909 ± 0.03	6.164GluLeu: 6.164 ± 0.03	1.713GluMet: 1.713 ± 0.011	2.863GluAsn: 2.863 ± 0.016	2.924GluPro: 2.924 ± 0.019	3.088GluGln: 3.088 ± 0.018	4.172GluArg: 4.172 ± 0.028	4.505GluSer: 4.505 ± 0.021	3.558GluThr: 3.558 ± 0.018	4.247GluVal: 4.247 ± 0.02	0.679GluTrp: 0.679 ± 0.007	1.526GluTyr: 1.526 ± 0.013	0.001GluXaa: 0.001 ± 0.0
Phe	1.901PheAla: 1.901 ± 0.012	1.03PheCys: 1.03 ± 0.008	1.762PheAsp: 1.762 ± 0.012	1.825PheGlu: 1.825 ± 0.011	1.57PhePhe: 1.57 ± 0.013	2.188PheGly: 2.188 ± 0.013	1.02PheHis: 1.02 ± 0.009	1.936PheIle: 1.936 ± 0.014	1.839PheLys: 1.839 ± 0.013	3.944PheLeu: 3.944 ± 0.022	0.807PheMet: 0.807 ± 0.008	1.525PheAsn: 1.525 ± 0.011	1.876PhePro: 1.876 ± 0.011	1.644PheGln: 1.644 ± 0.009	1.941PheArg: 1.941 ± 0.014	3.726PheSer: 3.726 ± 0.022	2.348PheThr: 2.348 ± 0.014	2.243PheVal: 2.243 ± 0.015	0.483PheTrp: 0.483 ± 0.007	1.237PheTyr: 1.237 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	3.986GlyAla: 3.986 ± 0.021	1.277GlyCys: 1.277 ± 0.011	3.239GlyAsp: 3.239 ± 0.018	4.163GlyGlu: 4.163 ± 0.025	2.483GlyPhe: 2.483 ± 0.016	5.14GlyGly: 5.14 ± 0.032	1.625GlyHis: 1.625 ± 0.012	2.508GlyIle: 2.508 ± 0.014	3.65GlyLys: 3.65 ± 0.021	5.446GlyLeu: 5.446 ± 0.024	1.375GlyMet: 1.375 ± 0.012	2.48GlyAsn: 2.48 ± 0.014	3.356GlyPro: 3.356 ± 0.039	2.747GlyGln: 2.747 ± 0.016	3.635GlyArg: 3.635 ± 0.019	5.921GlySer: 5.921 ± 0.032	3.278GlyThr: 3.278 ± 0.016	3.952GlyVal: 3.952 ± 0.018	0.76GlyTrp: 0.76 ± 0.008	1.769GlyTyr: 1.769 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.285HisAla: 1.285 ± 0.01	0.755HisCys: 0.755 ± 0.008	0.961HisAsp: 0.961 ± 0.008	1.165HisGlu: 1.165 ± 0.009	1.056HisPhe: 1.056 ± 0.008	1.528HisGly: 1.528 ± 0.012	1.029HisHis: 1.029 ± 0.012	1.27HisIle: 1.27 ± 0.01	1.331HisLys: 1.331 ± 0.01	2.734HisLeu: 2.734 ± 0.014	0.753HisMet: 0.753 ± 0.012	1.017HisAsn: 1.017 ± 0.008	1.554HisPro: 1.554 ± 0.012	1.322HisGln: 1.322 ± 0.01	1.661HisArg: 1.661 ± 0.011	2.442HisSer: 2.442 ± 0.016	1.628HisThr: 1.628 ± 0.017	1.444HisVal: 1.444 ± 0.011	0.333HisTrp: 0.333 ± 0.004	0.825HisTyr: 0.825 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.351IleAla: 2.351 ± 0.013	1.135IleCys: 1.135 ± 0.01	1.994IleAsp: 1.994 ± 0.013	2.288IleGlu: 2.288 ± 0.014	1.828IlePhe: 1.828 ± 0.015	2.248IleGly: 2.248 ± 0.014	1.324IleHis: 1.324 ± 0.014	2.359IleIle: 2.359 ± 0.016	2.609IleLys: 2.609 ± 0.015	4.238IleLeu: 4.238 ± 0.019	1.002IleMet: 1.002 ± 0.008	1.944IleAsn: 1.944 ± 0.012	2.409IlePro: 2.409 ± 0.013	2.2IleGln: 2.2 ± 0.013	2.498IleArg: 2.498 ± 0.013	3.833IleSer: 3.833 ± 0.016	2.693IleThr: 2.693 ± 0.018	2.486IleVal: 2.486 ± 0.015	0.476IleTrp: 0.476 ± 0.005	1.359IleTyr: 1.359 ± 0.01	0.001IleXaa: 0.001 ± 0.0
Lys	3.761LysAla: 3.761 ± 0.021	1.083LysCys: 1.083 ± 0.01	3.266LysAsp: 3.266 ± 0.018	4.751LysGlu: 4.751 ± 0.029	1.614LysPhe: 1.614 ± 0.011	3.215LysGly: 3.215 ± 0.024	1.469LysHis: 1.469 ± 0.011	2.552LysIle: 2.552 ± 0.015	4.521LysLys: 4.521 ± 0.029	5.077LysLeu: 5.077 ± 0.022	1.483LysMet: 1.483 ± 0.011	2.367LysAsn: 2.367 ± 0.014	3.148LysPro: 3.148 ± 0.023	2.651LysGln: 2.651 ± 0.016	3.532LysArg: 3.532 ± 0.019	4.087LysSer: 4.087 ± 0.025	3.395LysThr: 3.395 ± 0.017	3.547LysVal: 3.547 ± 0.02	0.583LysTrp: 0.583 ± 0.007	1.482LysTyr: 1.482 ± 0.01	0.002LysXaa: 0.002 ± 0.0
Leu	5.879LeuAla: 5.879 ± 0.023	2.248LeuCys: 2.248 ± 0.015	4.886LeuAsp: 4.886 ± 0.021	6.38LeuGlu: 6.38 ± 0.036	3.426LeuPhe: 3.426 ± 0.02	5.045LeuGly: 5.045 ± 0.02	2.733LeuHis: 2.733 ± 0.015	3.827LeuIle: 3.827 ± 0.018	5.791LeuLys: 5.791 ± 0.023	10.12LeuLeu: 10.12 ± 0.049	2.075LeuMet: 2.075 ± 0.013	3.673LeuAsn: 3.673 ± 0.018	5.322LeuPro: 5.322 ± 0.024	5.612LeuGln: 5.612 ± 0.027	5.634LeuArg: 5.634 ± 0.024	8.465LeuSer: 8.465 ± 0.029	5.388LeuThr: 5.388 ± 0.021	5.441LeuVal: 5.441 ± 0.023	1.042LeuTrp: 1.042 ± 0.009	2.525LeuTyr: 2.525 ± 0.013	0.001LeuXaa: 0.001 ± 0.0
Met	1.824MetAla: 1.824 ± 0.011	0.462MetCys: 0.462 ± 0.006	1.381MetAsp: 1.381 ± 0.01	1.947MetGlu: 1.947 ± 0.012	0.862MetPhe: 0.862 ± 0.007	1.337MetGly: 1.337 ± 0.012	0.472MetHis: 0.472 ± 0.006	0.873MetIle: 0.873 ± 0.007	1.521MetLys: 1.521 ± 0.011	2.004MetLeu: 2.004 ± 0.014	0.698MetMet: 0.698 ± 0.009	0.905MetAsn: 0.905 ± 0.008	1.033MetPro: 1.033 ± 0.011	0.955MetGln: 0.955 ± 0.009	1.222MetArg: 1.222 ± 0.01	1.876MetSer: 1.876 ± 0.011	1.252MetThr: 1.252 ± 0.009	1.458MetVal: 1.458 ± 0.01	0.257MetTrp: 0.257 ± 0.004	0.602MetTyr: 0.602 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.133AsnAla: 2.133 ± 0.013	0.898AsnCys: 0.898 ± 0.008	1.679AsnAsp: 1.679 ± 0.012	2.107AsnGlu: 2.107 ± 0.012	1.456AsnPhe: 1.456 ± 0.01	2.715AsnGly: 2.715 ± 0.016	1.019AsnHis: 1.019 ± 0.008	2.173AsnIle: 2.173 ± 0.012	2.313AsnLys: 2.313 ± 0.012	3.779AsnLeu: 3.779 ± 0.016	1.051AsnMet: 1.051 ± 0.009	1.834AsnAsn: 1.834 ± 0.013	2.285AsnPro: 2.285 ± 0.014	1.862AsnGln: 1.862 ± 0.012	2.066AsnArg: 2.066 ± 0.012	3.294AsnSer: 3.294 ± 0.017	2.197AsnThr: 2.197 ± 0.014	2.33AsnVal: 2.33 ± 0.012	0.461AsnTrp: 0.461 ± 0.005	1.123AsnTyr: 1.123 ± 0.008	0.001AsnXaa: 0.001 ± 0.0
Pro	4.279ProAla: 4.279 ± 0.023	1.092ProCys: 1.092 ± 0.014	2.972ProAsp: 2.972 ± 0.014	3.79ProGlu: 3.79 ± 0.02	1.927ProPhe: 1.927 ± 0.016	4.209ProGly: 4.209 ± 0.048	1.474ProHis: 1.474 ± 0.011	1.828ProIle: 1.828 ± 0.013	2.598ProLys: 2.598 ± 0.021	4.852ProLeu: 4.852 ± 0.021	0.989ProMet: 0.989 ± 0.008	1.974ProAsn: 1.974 ± 0.013	5.395ProPro: 5.395 ± 0.041	2.65ProGln: 2.65 ± 0.019	2.735ProArg: 2.735 ± 0.014	5.627ProSer: 5.627 ± 0.027	3.067ProThr: 3.067 ± 0.019	3.779ProVal: 3.779 ± 0.021	0.528ProTrp: 0.528 ± 0.006	1.374ProTyr: 1.374 ± 0.01	0.001ProXaa: 0.001 ± 0.0
Gln	3.097GlnAla: 3.097 ± 0.017	0.958GlnCys: 0.958 ± 0.01	2.33GlnAsp: 2.33 ± 0.013	3.543GlnGlu: 3.543 ± 0.021	1.368GlnPhe: 1.368 ± 0.009	2.624GlnGly: 2.624 ± 0.019	1.374GlnHis: 1.374 ± 0.01	1.973GlnIle: 1.973 ± 0.014	2.789GlnLys: 2.789 ± 0.017	4.558GlnLeu: 4.558 ± 0.024	1.146GlnMet: 1.146 ± 0.009	1.94GlnAsn: 1.94 ± 0.011	2.59GlnPro: 2.59 ± 0.021	3.374GlnGln: 3.374 ± 0.034	3.138GlnArg: 3.138 ± 0.019	3.604GlnSer: 3.604 ± 0.017	2.68GlnThr: 2.68 ± 0.014	2.751GlnVal: 2.751 ± 0.012	0.537GlnTrp: 0.537 ± 0.006	1.169GlnTyr: 1.169 ± 0.009	0.001GlnXaa: 0.001 ± 0.0
Arg	3.364ArgAla: 3.364 ± 0.015	1.317ArgCys: 1.317 ± 0.012	2.9ArgAsp: 2.9 ± 0.017	3.828ArgGlu: 3.828 ± 0.022	2.088ArgPhe: 2.088 ± 0.013	3.455ArgGly: 3.455 ± 0.02	1.606ArgHis: 1.606 ± 0.012	2.44ArgIle: 2.44 ± 0.013	3.665ArgLys: 3.665 ± 0.018	5.34ArgLeu: 5.34 ± 0.021	1.268ArgMet: 1.268 ± 0.009	2.174ArgAsn: 2.174 ± 0.013	2.998ArgPro: 2.998 ± 0.017	2.641ArgGln: 2.641 ± 0.016	4.496ArgArg: 4.496 ± 0.027	4.76ArgSer: 4.76 ± 0.027	2.983ArgThr: 2.983 ± 0.016	3.272ArgVal: 3.272 ± 0.017	0.689ArgTrp: 0.689 ± 0.007	1.529ArgTyr: 1.529 ± 0.009	0.001ArgXaa: 0.001 ± 0.0
Ser	5.859SerAla: 5.859 ± 0.025	2.234SerCys: 2.234 ± 0.02	4.543SerAsp: 4.543 ± 0.02	5.132SerGlu: 5.132 ± 0.023	3.288SerPhe: 3.288 ± 0.017	5.774SerGly: 5.774 ± 0.026	2.273SerHis: 2.273 ± 0.015	3.293SerIle: 3.293 ± 0.016	4.179SerLys: 4.179 ± 0.022	8.432SerLeu: 8.432 ± 0.029	1.747SerMet: 1.747 ± 0.011	3.137SerAsn: 3.137 ± 0.015	6.013SerPro: 6.013 ± 0.037	3.955SerGln: 3.955 ± 0.02	4.781SerArg: 4.781 ± 0.025	10.863SerSer: 10.863 ± 0.059	4.702SerThr: 4.702 ± 0.023	5.607SerVal: 5.607 ± 0.024	1.022SerTrp: 1.022 ± 0.01	2.192SerTyr: 2.192 ± 0.012	0.001SerXaa: 0.001 ± 0.0
Thr	3.982ThrAla: 3.982 ± 0.017	1.419ThrCys: 1.419 ± 0.017	2.847ThrAsp: 2.847 ± 0.012	3.807ThrGlu: 3.807 ± 0.018	2.148ThrPhe: 2.148 ± 0.012	3.765ThrGly: 3.765 ± 0.019	1.39ThrHis: 1.39 ± 0.012	2.362ThrIle: 2.362 ± 0.014	2.756ThrLys: 2.756 ± 0.018	5.28ThrLeu: 5.28 ± 0.017	1.178ThrMet: 1.178 ± 0.009	1.992ThrAsn: 1.992 ± 0.012	3.536ThrPro: 3.536 ± 0.022	2.35ThrGln: 2.35 ± 0.015	2.506ThrArg: 2.506 ± 0.014	4.894ThrSer: 4.894 ± 0.024	3.222ThrThr: 3.222 ± 0.023	4.114ThrVal: 4.114 ± 0.021	0.673ThrTrp: 0.673 ± 0.007	1.4ThrTyr: 1.4 ± 0.01	0.001ThrXaa: 0.001 ± 0.0
Val	4.066ValAla: 4.066 ± 0.017	1.744ValCys: 1.744 ± 0.013	3.125ValAsp: 3.125 ± 0.016	4.059ValGlu: 4.059 ± 0.024	2.666ValPhe: 2.666 ± 0.017	3.457ValGly: 3.457 ± 0.018	1.584ValHis: 1.584 ± 0.013	2.944ValIle: 2.944 ± 0.017	3.677ValLys: 3.677 ± 0.023	6.248ValLeu: 6.248 ± 0.022	1.48ValMet: 1.48 ± 0.009	2.406ValAsn: 2.406 ± 0.013	3.279ValPro: 3.279 ± 0.015	2.83ValGln: 2.83 ± 0.014	3.23ValArg: 3.23 ± 0.015	5.427ValSer: 5.427 ± 0.021	3.944ValThr: 3.944 ± 0.022	4.31ValVal: 4.31 ± 0.02	0.758ValTrp: 0.758 ± 0.007	1.773ValTyr: 1.773 ± 0.012	0.0ValXaa: 0.0 ± 0.0
Trp	0.648TrpAla: 0.648 ± 0.006	0.25TrpCys: 0.25 ± 0.004	0.632TrpAsp: 0.632 ± 0.007	0.729TrpGlu: 0.729 ± 0.007	0.476TrpPhe: 0.476 ± 0.006	0.604TrpGly: 0.604 ± 0.008	0.263TrpHis: 0.263 ± 0.004	0.589TrpIle: 0.589 ± 0.007	0.737TrpLys: 0.737 ± 0.007	1.137TrpLeu: 1.137 ± 0.01	0.34TrpMet: 0.34 ± 0.005	0.499TrpAsn: 0.499 ± 0.007	0.423TrpPro: 0.423 ± 0.005	0.468TrpGln: 0.468 ± 0.005	0.753TrpArg: 0.753 ± 0.008	0.983TrpSer: 0.983 ± 0.01	0.733TrpThr: 0.733 ± 0.008	0.676TrpVal: 0.676 ± 0.007	0.19TrpTrp: 0.19 ± 0.004	0.345TrpTyr: 0.345 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.387TyrAla: 1.387 ± 0.009	0.73TyrCys: 0.73 ± 0.008	1.34TyrAsp: 1.34 ± 0.01	1.522TyrGlu: 1.522 ± 0.01	1.184TyrPhe: 1.184 ± 0.009	1.618TyrGly: 1.618 ± 0.011	0.786TyrHis: 0.786 ± 0.007	1.409TyrIle: 1.409 ± 0.012	1.455TyrLys: 1.455 ± 0.009	2.559TyrLeu: 2.559 ± 0.014	0.641TyrMet: 0.641 ± 0.006	1.171TyrAsn: 1.171 ± 0.009	1.292TyrPro: 1.292 ± 0.009	1.231TyrGln: 1.231 ± 0.009	1.655TyrArg: 1.655 ± 0.012	2.332TyrSer: 2.332 ± 0.014	1.55TyrThr: 1.55 ± 0.011	1.55TyrVal: 1.55 ± 0.012	0.381TyrTrp: 0.381 ± 0.006	0.947TyrTyr: 0.947 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.004

Statistics based on 30440 proteins (17431430 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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